Mediaspace scheduled maintenance: Aug 25, 2026 07:00 - 12:00 AM. During this time, videos will be temporarily unavailable. Check status updates.

Concept

Centrifugation

Publications associées (30)

Single-Chain Nanoparticles for Intracellular Drug Delivery

Suiyang Liao

Single-chain nanoparticles (SCNPs) are a type of polymeric nanoparticles formed by collapsing/folding individual polymer chains via intra-molecular interactions. Their emergence holds promise to construct sub-20 nm polymeric nanoparticles with defined stru ...

EPFL2022

2.4-angstrom structure of the double-ring Gemmatimonas phototrophica photosystem

Henning Paul-Julius Stahlberg

Phototrophic Gemmatimonadetes evolved the ability to use solar energy following horizontal transfer of photosynthesis-related genes from an ancient phototrophic proteobacterium. The electron cryo-microscopy structure of the Gemmatimonas phototrophica photo ...

AMER ASSOC ADVANCEMENT SCIENCE2022

Techno-economic analysis and life cycle assessment of a biorefinery utilizing reductive catalytic fractionation

Jeremy Luterbacher

Reductive catalytic fractionation (RCF) is a promising approach to fractionate lignocellulose and convert lignin to a narrow product slate. To guide research towards commercialization, cost and sustainability must be considered. Here we report a techno-eco ...

2021

Control and Characterization of the Compactness of Single-Chain Nanoparticles

Francesco Stellacci, Luciano Andres Abriata, Lixia Wei, Suiyang Liao

Polymers when self-cross-linked into single-chain nanoparticles bear some resemblance to folded proteins; yet proteins have clear energy landscapes that determine precisely folded structures, while single-chain polymer nanoparticles (SCNPs) have more undef ...

American Chemical Society2021

An Instrumented High-Speed Rotor with Embedded Telemetry for The Continuous Spatial Pressure Profile Measurement in Gas Lubricated Bearings: A Proof of Concept

Jürg Alexander Schiffmann, Karim Magdi Zakaria Abdelrahman Shalash

Pressure is one of the constitutional quantities governing the flow field in gas lubricated bearings. Knowledge of the pressure is of principal importance in the fundamental understanding of such bearings and for the calibration of reduced order models. Th ...

2020

A new class of protein biomarkers based on subcellular distribution: application to a mouse liver cancer model

Kristina Schoonjans, Evan Graehl Williams, Pan Xu, Vera Monica Lemos Da Silva, Amir Banaei Esfahani

To-date, most proteomic studies aimed at discovering tissue-based cancer biomarkers have compared the quantity of selected proteins between case and control groups. However, proteins generally function in association with other proteins to form modules loc ...

Nature Research2019

On the effect of ligand shell heterogeneity on nanoparticle/protein binding thermodynamics

Francesco Stellacci, Ahmet Bekdemir, Suiyang Liao

Nonspecific protein adhesion to nanoparticle (NP) has been proven to have important implications in nanomedicine. However, there are only a few examples of careful studies relating protein binding thermodynamics to NP physicochemical features. In particula ...

2019

Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells

Florian Maria Wurm, Bernard Schneider, Nicole Déglon, Vivianne Padrun

Adeno-associated virus (AAV) vectors are currently among the most commonly applied for in vivo gene therapy approaches. The evaluation of vectors during clinical development requires the production of considerable amounts of highly pure and potent vectors. ...

2019

Separation of blood microsamples by exploiting sedimentation at the microscale

Philippe Renaud, David Forchelet

Microsample analysis is highly bene cial in blood-based testing where cutting-edge bioanalytical technologies enable the analysis of volumes down to a few tens of microliters. Despite the availability of analytical methods, the di culty in obtaining high-q ...

Nature Research2018

Microdevice for Sedimentation-based Separation of Blood Microsamples at the Point of Collection

David Forchelet

Laboratory blood testing plays a central role in current diagnostics and therapeutical decisions. Thus, errors have a direct impact on care quality and cost. The majority of errors occur in the pre-analytical phase, when samples are collected, stored and p ...

EPFL2017

Generation and Characterization of Anti-VGLUT Nanobodies Acting as Inhibitors of Transport

The uptake of glutamate by synaptic vesicles is mediated by vesicular glutamate transporters (VGLUTs). The central role of these transporters in excitatory neurotransmission underpins their importance as pharmacological targets. Although several compounds ...

Amer Chemical Soc2017

Mercury tissue residue approach in Chironomus riparius: Involvement of toxicokinetics and comparison of subcellular fractionation methods

Along with the growing body of evidence that total internal concentration is not a good indicator of toxicity, the Critical Body Residue (CBR) approach recently evolved into the Tissue Residue Approach (TRA) which considers the biologically active portion ...

Elsevier Science Bv2016

A centrifugation-based physicochemical characterization method for the interaction between proteins and nanoparticles

Francesco Stellacci, Ahmet Bekdemir

Nanomedicine requires in-depth knowledge of nanoparticle-protein interactions. These interactions are studied with methods limited to large or fluorescently labelled nanoparticles as they rely on scattering or fluorescence-correlation signals. Here, we hav ...

Nature Publishing Group2016

Viral RNA Degradation and Diffusion Act as a Bottleneck for the Influenza A Virus Infection Efficiency

Christian Sieben

After endocytic uptake, influenza viruses transit early endosomal compartments and eventually reach late endosomes. There, the viral glycoprotein hemagglutinin (HA) triggers fusion between endosomal and viral membrane, a critical step that leads to release ...

Public Library Science2016

Numerical study on the suitability of centrifuge testing for capturing the thermal-induced mechanical behavior of energy piles

Lyesse Laloui, Alessandro Francesco Rotta Loria, Alice Di Donna

Currently, the suitability of centrifuge testing for capturing the thermal-induced mechanical behavior of full-scale energy piles has not been established, yet, despite their increasing worldwide development. Looking at this challenge, this paper investiga ...

American Society of Civil Engineers2015

A Multilaboratory Comparison of Calibration Accuracy and the Performance of External References in Analytical Ultracentrifugation

Jutta Christine Wandrey, Ahmet Bekdemir

Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules and their complexes, reporting on their size and shape in free solution. The purpose of this ...

Public Library of Science2015

Centrifuge modelling of heating effects on energy pile performance in saturated sand

Lyesse Laloui

The operation of energy pile in summer can expel excessive heat of the buildings into the ground by the use of a heat pump. Despite of having been implemented for decades, the design of energy pile still relies heavily on empiricism as there is still limit ...

National Research Council Canada2015

Interaction of Silver Nanoparticles (AgNP) with Rainbow Trout Gill Cell Lines

Yang Yue

Owing to their unique antimicrobial properties, silver nanoparticles (AgNP) are among the most widely used engineered nanoparticles in a variety of consumer products and medical applications. Their resulting release into the aquatic environment raises conc ...

EPFL2015

Sedimentation of macroscopic rigid knots and its relation to gel electrophoretic mobility of DNA knots

Giovanni Dietler, Cédric Weber

We address the general question of the extent to which the hydrodynamic behaviour of microscopic freely fluctuating objects can be reproduced by macrosopic rigid objects. In particular, we compare the sedimentation speeds of knotted DNA molecules undergoin ...

Nature Publishing Group2013

Flow simulation and hemolysis modeling for a blood centrifuge device

Mark Sawley, Michel Deville, Patric Hagmann

A common blood separation technique is to accelerate the sedimentation of blood constituents by centrifugation and then collect these constituents separately. When subjected to conditions of excessive stress, hemolysis can however occur, whereby the membra ...

Pergamon-Elsevier Science Ltd2013