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Concept

Optical sectioning

Publications associées (31)

3D nanometrology of transparent objects by phase calibration of a basic bright-field microscope for multiple illumination apertures

Martinus Adela Maria Gijs, Daniel Migliozzi

Optical retrieval of the structure of transparent objects at the nanoscale requires adapted techniques capable of probing their interaction with light. Here, we considered a method based on calibration of the defocusing with partially coherent illumination ...

2020

Development of Sample-Adaptable Holders for Lightsheet Microscopy

Olivier Burri, Arne Seitz, Thierry Laroche, Lalit Kumar Dubey

Multi-user core microscopy facilities are often faced with the challenge to adapt or modify existing instruments. This is essential in order to fulfill the requirements of the user community, who wants to image a wide range of model organisms with varying ...

2019

Simultaneous dual-contrast three-dimensional imaging in live cells via optical diffraction tomography and fluorescence

Pierre Marquet, Christian Depeursinge, Chen Liu

We report a dual-contrast method of simultaneously measuring and visualizing the volumetric structural information in live biological samples in three-dimensional (3D) space. By introducing a direct way of deriving the 3D scattering potential of the object ...

2019

Digital staining through the application of deep neural networks to multi-modal multi-photon microscopy

Demetri Psaltis, Navid Borhani

Deep neural networks have been used to map multi-modal, multi-photon microscopy measurements of a label-free tissue sample to its corresponding histologically stained brightfield microscope colour image. It is shown that the extra structural and functional ...

2019

Endogenous SHG and 2PEF coherence imaging of substructures in neurons in 3D

Sylvie Roke, Carlos Alberto Macias Romero, Marie Eve Pascale Didier, Claire Jacqueline Léa Teulon

Neuronal morphology, long-distance transport and signalling critically depend on the organization of microtubules in the cytoskeleton. Second harmonic generation (SHG) imaging has been recognized as a potentially powerful tool for in situ label-free neuroi ...

2019

Waveguide-Based Platform for Large-FOV Imaging of Optically Active Defects in 2D Materials

Aleksandra Radenovic, Suliana Manley, Martina Lihter, Evgenii Glushkov, Vytautas Navikas, Anton Stroganov, Anna Archetti, Mukeshchand Thakur, Jean Camille Benjamin Comtet, Juan Francisco Gonzalez Marin

Single-molecule localization microscopy (SMLM) is a powerful tool that is routinely used for nanoscale optical imaging of biological samples. Recently, this approach has been applied to study optically active defects in two-dimensional (2D) materials. Such ...

AMER CHEMICAL SOC2019

Scanning near-field optical microscopy with new probes and feedback modes

Anton Smirnov

Scanning Near-field Optical Microscopy (SNOM) technique enables to overcome Abbe diffraction limit of far-field optics as well as to obtain simultaneously optical and topographical images. While the optical resolution of the method is limited by the apertu ...

EPFL2019

Phase resolved characterization of nanoscale features in optical microstructures

Michail Symeonidis

In this study, we apply interferometric microscopy to study the phase, alongside the intensity, of the light field transmitted through a wide variety of samples. Additionally, we conduct those interferometric measurements at different wavelengths within th ...

EPFL2019

Photocarrier-induced band-gap renormalization and ultrafast charge dynamics in black phosphorus

Majed Chergui, Marco Grioni, Alberto Crepaldi, Michele Puppin, Gianmarco Gatti, Steven Roth

With its tunable band-gap and its unique optical and electronic properties black phosphorus (BP) opens exciting opportunities for optoelectronic nanotechnology. The band-gap extends from the visible to the mid-infrared spectral range, as a function of samp ...

2019

Imaging tissues and cells beyond the diffraction limit with structured illumination microscopy and Bayesian image reconstruction

Tomas Lukes

Background: Structured illumination microscopy (SIM) is a family of methods in optical fluorescence microscopy that can achieve both optical sectioning and super-resolution effects. SIM is a valuable method for high-resolution imaging of fixed cells or tis ...

Oxford University Press (OUP)2019

GlobalBioIm: A Unifying Computational Framework for Solving Inverse Problems

Michaël Unser, Michael Thompson McCann, Emmanuel Emilien Louis Soubies, Ferréol Arnaud Marie Soulez, Laurène Donati

We present a unifying framework for the development of state-of-the-art reconstruction algorithms in computational optics with a clear separation between the physical (forward model) and signal-related (regularization, incorporation of prior constraints) a ...

OSA2017