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Concept

Clonage thérapeutique

Publications associées (31)

Engineering Dynamic Hydrogels For In Vitro Organoid Culture

Delphine Lorraine Perle Blondel

Over the past decade, methods to culture stem cells in three dimensions have opened up a plethora of new opportunities for basic and translational research in the life sciences. In particular, the use of natural extracellular matrix (ECM) surrogates, such ...

EPFL2019

A Kinetic Map of the Homomeric Voltage-Gated Potassium Channel (Kv) Family

Henry Markram, Rajnish Ranjan, Emmanuelle Logette, Enrico Scantamburlo, Michela Marani, Valérie Buchillier, Mirjia Herzog

The voltage-gated potassium (Kv) channels, encoded by 40 genes, repolarize all electrically excitable cells, including plant, cardiac, and neuronal cells. Although these genes were fully sequenced decades ago, a comprehensive kinetic characterization of al ...

2019

Hominoid-Specific Transposable Elements and KZFPs Facilitate Human Embryonic Genome Activation and Control Transcription in Naive Human ESCs

Didier Trono, Priscilla Turelli, Sandra Eloise Kjeldsen, Evaristo Jose Planet Letschert, Julien Léonard Duc, Alexandre Coudray, Julien Paul André Pontis

Expansion of transposable elements (TEs) coincides with evolutionary shifts in gene expression. TEs frequently harbor binding sites for transcriptional regulators, thus enabling coordinated genome-wide activation of species- and context-specific gene expre ...

2019

Anatomy and Physiology of the Digestive Tract of Drosophila melanogaster

Bruno Lemaitre

The gastrointestinal tract has recently come to the forefront of multiple research fields. It is now recognized as a major source of signals modulating food intake, insulin secretion and energy balance. It is also a key player in immunity and, through its ...

GENETICS SOCIETY AMERICA2018

Bimodal CD40/Fas-Dependent Crosstalk between iNKT Cells and Tumor-Associated Macrophages Impairs Prostate Cancer Progression

Michele De Palma

Heterotypic cellular and molecular interactions in the tumor microenvironment (TME) control cancer progression. Here, we show that CD1d-restricted invariant natural killer (iNKT) cells control prostate cancer (PCa) progression by sculpting the TME. In a mo ...

2018

Cross-Tissue Identification of Somatic Stem and Progenitor Cells Using a Single-Cell RNA-Sequencing Derived Gene Signature

Bart Deplancke, Jörg Hülsken, Petra Catalina Schwalie

A long-standing question in biology is whether multipotent somatic stem and progenitor cells (SSPCs) feature molecular properties that could guide their system-independent identification. Population-based transcriptomic studies have so far not been able to ...

Wiley2017

Single cell isolation process with laser induced forward transfer

Philippe Renaud, Yu Deng

Background A viable single cell is crucial for studies of single cell biology. In this paper, laser-induced forward transfer (LIFT) was used to isolate individual cell with a closed chamber designed to avoid contamination and maintain humidity. Hela cells ...

Biomed Central Ltd2017

Versatility of Pyridoxal Phosphate as a Coating of Iron Oxide Nanoparticles

Heinrich Hofmann, Josefina Adriana Maria Bastiaansen, Ulrich Aschauer, Marijana Mionic Ebersold, Débora Bonvin

Pyridoxal 5'-phosphate (PLP) is the most important cofactor of vitamin B-6-dependent enzymes, which catalyses a wide range of essential body functions (e.g., metabolism) that could be exploited to specifically target highly metabolic cells, such as tumour ...

Mdpi Ag2017

The Differential Distribution of RAPTA-T in Non-Invasive and Invasive Breast Cancer Cells Correlates with Its Anti-Invasive and Anti-Metastatic Effects

Paul Joseph Dyson, Anders Meibom, Graham Knott, Stéphane Laurent Escrig, Catherine Maclachlan

Nanoscale secondary ion mass spectrometry (NanoSIMS) combined with transmission electron microscopy (TEM) can be a powerful approach to visualize the exact distribution of drugs at the sub-cellular level. In this work, we exploit this approach to identify ...

2017

Cell lineage tracing in human tissues using somatic mitochondrial mutations

Paul Refinetti

Lineage tracing is a basic concept in developmental biology, as well as diseases related to organ development. A reliable method to track cell lineages in human tissues has the potential to greatly contribute to the fields of oncogenesis, tissue renewal, a ...

EPFL2017

Perfluorinated HDAC inhibitors as selective anticancer agents

Paul Joseph Dyson, Tina Riedel

A series of potent histone deacetylase inhibitors is presented that incorporate alkyl or perfluorinated alkyl chains. Several new compounds show greater in vitro antiproliferative activity than the clinically approved inhibitor, SAHA. Furthermore, the new ...

Royal Society of Chemistry2017