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Catégorie

Chromatographie

Publications associées (32)

iSort enables automated complex microfluidic droplet sorting in an effort to democratize technology

Christoph Merten, Jatin Panwar

Fluorescence-activated droplet sorting (FADS) is a widely used microfluidic technique for high-throughput screening. However, it requires highly trained specialists to determine optimal sorting parameters, and this results in a large combinatorial space th ...

CELL PRESS2023

Label-free plasmonic microarray for multiplexed analysis of cells and tumor organoids

Yen-Cheng Liu

Investigating the dynamic activities of protein expression and signaling in living organisms is a crucial focus of intense research aimed at elucidating the processes that underlie disease progression and improving treatments and drug development. Resolvin ...

EPFL2023

Cavitation induced by pulsed and continuous-wave fiber lasers in confinement

Christophe Moser, Jan Krizek

Bubbles generated with lasers in liquids under confinement have been investigated for their potential use as the driving mechanism for liquid micro-jets in various microfluidic devices, such as needle-free jet injectors. Here, we report on the study of bub ...

ELSEVIER SCIENCE INC2023

Screening for drivers of SARS-CoV-2 uptake

Christoph Merten, Alexis Louis Autour

Genetic and cellular drivers of the cellular uptake of SARS-CoV-2 can be screened at high throughput via droplet microfluidics and size-exclusion methods for the analysis of the formation of fusions between cells expressing the virus's spike protein and ce ...

Nature Portfolio2023

Single-Cell Optical Nanomotion of Candida albicans in Microwells for Rapid Antifungal Susceptibility Testing

Sandor Kasas, María Inés Villalba

Candida albicans is an emerging multidrug-resistant opportunistic pathogen representing an important source of invasive disease in humans and generating high healthcare costs worldwide. The development of a rapid and simple antifungal susceptibility test ( ...

MDPI2023

Fluorescence crosstalk reduction by modulated excitation-synchronous acquisition for multispectral analysis in high-throughput droplet microfluidics

Christoph Merten, Jatin Panwar

Crosstalk between fluorescent biomarkers significantly limits the resolution of multispectral fluorescence analysis in real-time droplet-microfluidics applications. The crosstalk is a result of overlapping emission and excitation spectra of different fluor ...

ROYAL SOC CHEMISTRY2023

Bacterial colonization in realistic environments: how mechanics impact biofilm formation in the wild and during infection

Tamara Rossy

A variety of physical inputs acts onto bacteria in nature. However, these are most often ignored in the studies of their physiology. There is now increasing evidence indicating that bacteria respond to physical stimuli, including mechanical forces. Yet, qu ...

EPFL2023

Microfluidic-assisted chromogenic in situ hybridization (MA-CISH) for fast and accurate breast cancer diagnosis

Martinus Adela Maria Gijs, Raphaël Etienne Jean Trouillon, Huu Tuan Nguyen

Chromogenic in situ hybridization (CISH) is a common technique that is used for human epidermal growth factor receptor 2 (HER2) assessment in breast cancer diagnosis. CISH is based on the enzymatic amplification of DNA markers, generating a colorimetric im ...

Elsevier2017

A micro gas preconcentrator with improved performance for pollution monitoring and explosives detection

Nico de Rooij, Danick Briand, El Hadji Malick Camara

This paper presents the optimization of a micro gas preconcentrator based on a micro-channel in porous and non-porous silicon filled with an adequate adsorbent. This micro gas preconcentrator is both applicable in the fields of atmospheric pollution monito ...

Elsevier2011

System for optical detection and imaging of sub-diffraction-limited nano-objects

Martinus Adela Maria Gijs, Matteo Cornaglia, Hui Yang

Nano-objects (8) with feature size smaller than the classical Abbe's diffraction limit can be optically detected via a microlens (4) array configured in a miniaturized microfluidic device. A hydrophilic microwell (3) array in a hydrophobic layer (2) is fir ...

2016

Hydrogel microfluidics for the patterning of pluripotent stem cells

Matthias Lütolf, Steffen Cosson

Biomolecular signaling is of utmost importance in governing many biological processes such as the patterning of the developing embryo where biomolecules regulate key cell-fate decisions. In vivo, these factors are presented in a spatiotemporally tightly co ...

Nature Publishing Group2014

A microchip emitter for solid phase extraction – gradient elution – mass spectrometry

Hubert Girault, Liang Qiao, Natalia Gasilova, Dmitry Momotenko, Mohammad Reza Pourhaghighi

A microchip electrospray emitter with a magnetic bead trap has been designed for solid phase extraction-gradient elution-mass spectrometry (SPE-GEMS). The goal of this method is the detection of analytes at low concentrations and it is here demonstrated us ...

American Chemical Society2013