Publications related to A microfluidic platform for automated phenotyping during full lifespan of Caenorhabditis elegans at high-throughput

Related publications (25)

Drug Screening in C. elegans to identify new pharmacological mitochondrial stress inducers

Mitochondria are essential organelles participating in numerous cellular functions, including energy harvesting, regulation of homeostasis and apoptosis. Changes in mitochondrial number, morphology, and function not only impact cellular metabolism but also ...

EPFL2024

Secondary structure of the human mitochondrial genome affects formation of deletions

Jacques Fellay, Konstantin Popadin

BackgroundAging in postmitotic tissues is associated with clonal expansion of somatic mitochondrial deletions, the origin of which is not well understood. Such deletions are often flanked by direct nucleotide repeats, but this alone does not fully explain ...

BMC2023

Detecting the metabolism of individual yeast mutant strain cells when aged, stressed or treated with antioxidants with diamond magnetometry

Mayeul Sylvain Chipaux

Free radicals play a key role in the ageing process. However, free radicals are small, reactive and short lived and thus challenging to measure. We utilize a new technique called diamond magnetometry for this pur-pose. We make use of nitrogen vacancy cente ...

ELSEVIER SCI LTD2023

V-ATPase/TORC1-mediated ATFS-1 translation directs mitochondrial UPR activation in C. elegans

Johan Auwerx, Kristina Schoonjans, Felix Naef, Xiaoxu Li, Yang Li, Hao Li, Jia Liu, Yan Liu, Wen Gao, Amélia Lalou, Nagammal Neelagandan

To adapt mitochondrial function to the ever-changing intra- and extracellular environment, multiple mitochondrial stress response (MSR) pathways, including the mitochondrial unfolded protein response (UPRmt), have evolved. However, how the mitochondrial st ...

Rockefeller University Press2022

Reduced ech-6 expression attenuates fat-induced lifespan shortening in C. elegans

Richardus Houtkooper, Yan Liu, Wen Gao

Deregulated energy homeostasis represents a hallmark of aging and results from complex gene-by-environment interactions. Here, we discovered that reducing the expression of the gene ech-6 encoding enoyl-CoA hydratase remitted fat diet-induced deleterious e ...

Nature Portfolio2022

Automated phenotyping of Caenorhabditis elegans embryos with a high-throughput-screening microfluidic platform

Martinus Adela Maria Gijs, Matteo Cornaglia, Raphaël Etienne Jean Trouillon, Hüseyin Baris Atakan

The nematode Caenorhabditis elegans has been extensively used as a model multicellular organism to study the influence of osmotic stress conditions and the toxicity of chemical compounds on developmental and motility-associated phenotypes. However, the sev ...

2020

High-throughput and adaptive super-resolution fluorescence microscopy of organelles

Dora Mahecic

Fluorescence super-resolution microscopy has allowed unprecedented insight into the workings of biological systems below the diffraction limit of light. Over the past decade, it has overcome several challenges to deliver 3D, multi-color and faster imaging ...

EPFL2020

Automated Platform for Long-Term Culture and High-Content Phenotyping of Single C. elegans Worms

Martinus Adela Maria Gijs, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Elena Katsyuba, Hüseyin Baris Atakan, Rongrong Xiang

The nematode Caenorhabditis elegans is a suitable model organism in drug screening. Traditionally worms are grown on agar plates, posing many challenges for long-term culture and phenotyping of animals under identical conditions. Microfluidics allows for ' ...

Nature Research2019

Long-term imaging, automated high-content phenotyping and sorting of C. elegans larvae and embryos using microfluidic platforms

Hüseyin Baris Atakan

C. elegans is frequently studied to address fundamental biological questions motivated by its short life cycle, small size, hermaphrodite behavior and well-annotated genome. In this work, we present novel microfluidic platforms that support long-term cultu ...

EPFL2019

The RNA-Binding Protein PUM2 Impairs Mitochondrial Dynamics and Mitophagy During Aging

Johan Auwerx, Bernard Schneider, Kristina Schoonjans, Graham Knott, Hao Li, Adrienne Joëlle Laurence Mottis, Vincenzo Sorrentino, Davide D'Amico, Mario Romani, Vera Monica Lemos Da Silva

Little information is available about how post-transcriptional mechanisms regulate the aging process. Here, we show that the RNA-binding protein Pumilio2 (PUM2), which is a translation repressor, is induced upon aging and acts as a negative regulator of li ...

CELL PRESS2019

Mitochondrial stress signalling and its impacts on physiology

Adrienne Joëlle Laurence Mottis

Mitochondria are responsible for respiration and energy harvesting across the eukaryotic kingdom. They also take part in other cellular processes like intermediary metabolism. As a result, mitochondrial function directly impacts organismal features such as ...

EPFL2018

Microfluidic high-content phenotypic screening on a Caenorhabditis elegans model

Maria Cristina Letizia

Caenorhabditis elegans is a performant model system for studying human biological processes and diseases and for pre-clinical phenotyping screenings of compounds. Microfluidics has been instrumental in enabling C. elegans-based drug assays, facilitating th ...

EPFL2018

Lab-on-a-chip technologies for manipulation and imaging of C. elegans worms and embryos

Li Dong

The nematode Caenorhabditis elegans is an attractive model organism, owing notably to its short life cycle, genetic tractability, and optical transparency facilitating microscopic observation. This thesis deals with the realization of technological tools f ...

EPFL2018

Systems Phytohormone Responses to Mitochondrial Proteotoxic Stress

Johan Auwerx, Xu Wang

Mitochondrial function is controlled by two separate genomes. This feature makes mitochondria prone to proteotoxic stress when a stoichiometric imbalance occurs in the protein complexes that perform oxidative phosphorylation, which consist of both nucleara ...

Cell Press2017

Enhancing mitochondrial proteostasis reduces amyloid-beta proteotoxicity

Johan Auwerx, Adrian Schmid, Norman Lionel Moullan, Hongbo Zhang, Vincenzo Sorrentino, Davide D'Amico, Mario Romani

Alzheimer's disease is a common and devastating disease characterized by aggregation of the amyloid-beta peptide. However, we know relatively little about the underlying molecular mechanisms or how to treat patients with Alzheimer's disease. Here we provid ...

2017

Microfluidic systems for high-throughput and high-content screening using the nematode Caenorhabditis elegans

Martinus Adela Maria Gijs, Thomas Lehnert, Matteo Cornaglia

In a typical high-throughput drug screening (HTS) process, up to millions of chemical compounds are applied to cells cultured in well plates, aiming to find molecules that exhibit a robust dose-response, as evidenced for example by a fluorescence signal. I ...

Royal Soc Chemistry2017

Two Conserved Histone Demethylases Regulate Mitochondrial Stress-Induced Longevity

Johan Auwerx, Laurent Mouchiroud, Evan Graehl Williams, Virginija Jovaisaite

Across eukaryotic species, mild mitochondrial stress can have beneficial effects on the lifespan of organisms. Mitochondrial dysfunction activates an unfolded protein response (UPR(mt)), a stress signaling mechanism designed to ensure mitochondrial homeost ...

Cell Press2016

On-chip microfluidic biocommunication assay for studying male-induced demise in C. elegans hermaphrodites

Martinus Adela Maria Gijs, Thomas Lehnert, Matteo Cornaglia, Li Dong

Like other animals, C. elegans nematodes have the ability to socially interact and to communicate through exchange and sensing of small soluble signaling compounds that help them cope with complex environmental conditions. For the time being, worm biocommu ...

Royal Soc Chemistry2016

Mitochondrial unfolded protein response, its molecular mechanism and physiological impact

Virginija Jovaisaite

Mitochondria are the main producers of ATP, the energy currency of the cell, and they perform a wide array of other fundamental functions. These organelles are essential not only for cellular metabolism, but also for organismal physiology and lifespan. A r ...

EPFL2016

Metabolic control of muscle and muscle stem cell function

Hongbo Zhang

Skeletal muscle composed of myofibers and a small amount of muscle stem cells (MuSCs). It plays important roles in energy metabolism. Some of the key metabolites, such as NAD+ and acetyl-CoA were recently found to regulate muscle and MuSCs function. Most o ...

EPFL2016