Publication

Process development for the production of monoclonal IgA antibodies that protect mucosal surfaces

Ian William Marison
1994
Journal Articles

Abstract

This paper describes the prodn. of IgA antibodies in a range of bioreactor configurations using FCS-contg. RPMI 1640 medium. Under std. batch STR conditions, a max. viable cell and IgA concns. of 9.5*105 cell mL-1 and 47 mg l-1 were obtained. Under fed-batch conditions, these values were increased by a factor of 60% and 155%, resp. IgA concn. was increased by a factor of more than 10-fold in a HFR. The reason for the low yields in suspension cultures is probably due to limitations of essential nutrients, such as the balance of amino acids. A defined serum- and protein-free medium has been developed to explore this in more detail. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/e0bb2f41-c389-49da-99a4-dd2325316984

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Ian William Marison
1994
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/e0bb2f41-c389-49da-99a4-dd2325316984

About this result

Related concepts (32)

Essential amino acid

An essential amino acid, or indispensable amino acid, is an amino acid that cannot be synthesized from scratch by the organism fast enough to supply its demand, and must therefore come from the diet. Of the 21 amino acids common to all life forms, the nine amino acids humans cannot synthesize are valine, isoleucine, leucine, methionine, phenylalanine, tryptophan, threonine, histidine, and lysine.

Cell culture

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. This technique is also called micropropagation. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. They need to be kept at body temperature (37 °C) in an incubator.

Antibody

An antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shaped protein used by the immune system to identify and neutralize foreign objects such as pathogenic bacteria and viruses. The antibody recognizes a unique molecule of the pathogen, called an antigen. Each tip of the "Y" of an antibody contains a paratope (analogous to a lock) that is specific for one particular epitope (analogous to a key) on an antigen, allowing these two structures to bind together with precision.

Related publications (4)

Process development for the production of monoclonal IgA antibodies that protect mucosal surfaces

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