Publication

Indirect biomass determination in case of non-constant metabolic rates

Urs von Stockar, Ian William Marison, Martin Rhiel
1999
Conference Papers

Abstract

A novel approach for indirect biomass detn. in case of non-const. specific metabolic rates is reported. Specific glucose consumption rate was detd. during a first calibration culture for both free suspended and immobilized cells. It was expressed as a linear function of the limiting substrate, i. e. glucose for suspension and oxygen for immobilized cell cultures. For validation, these time-independent models were applied on a second culture presenting a different inoculum cell d. Online monitoring of the limiting substrate enabled a continuous detn. of the specific metabolic rate and thus online cell no. prediction, which was verified with an off-line cell counting technique. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/d0247de7-63b5-4e57-88c9-8e77d2bf3d4b

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Urs von Stockar, Ian William Marison, Martin Rhiel
1999
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/d0247de7-63b5-4e57-88c9-8e77d2bf3d4b

About this result

Related concepts (32)

Resting metabolic rate

Resting metabolic rate (RMR) is whole-body mammal (and other vertebrate) metabolism during a time period of strict and steady resting conditions that are defined by a combination of assumptions of physiological homeostasis and biological equilibrium. RMR differs from basal metabolic rate (BMR) because BMR measurements must meet total physiological equilibrium whereas RMR conditions of measurement can be altered and defined by the contextual limitations.

Basal metabolic rate

Basal metabolic rate (BMR) is the rate of energy expenditure per unit time by endothermic animals at rest. It is reported in energy units per unit time ranging from watt (joule/second) to ml O2/min or joule per hour per kg body mass J/(h·kg). Proper measurement requires a strict set of criteria to be met. These criteria include being in a physically and psychologically undisturbed state and being in a thermally neutral environment while in the post-absorptive state (i.e., not actively digesting food).

Glucose

Glucose is a sugar with the molecular formula . Glucose is overall the most abundant monosaccharide, a subcategory of carbohydrates. Glucose is mainly made by plants and most algae during photosynthesis from water and carbon dioxide, using energy from sunlight, where it is used to make cellulose in cell walls, the most abundant carbohydrate in the world. In energy metabolism, glucose is the most important source of energy in all organisms. Glucose for metabolism is stored as a polymer, in plants mainly as starch and amylopectin, and in animals as glycogen.

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Indirect biomass determination in case of non-constant metabolic rates

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