Publications related to Generation and Characterization of Anti-VGLUT Nanobodies Acting as Inhibitors of Transport

Related publications (30)

A bistable inhibitory optoGPCR for multiplexed optogenetic control of neural circuits

Information is transmitted between brain regions through the release of neurotransmitters from long-range projecting axons. Understanding how the activity of such long-range connections contributes to behavior requires efficient methods for reversibly mani ...

Nature Publishing Group2024

Assemblies, synapse clustering and network topology interact with plasticity to explain structure-function relationships of the cortical connectome

Eilif Benjamin Muller, Michael Reimann, Marwan Muhammad Ahmed Abdellah, James Gonzalo King, András Ecker, Pramod Shivaji Kumbhar, Giuseppe Chindemi, Daniela Egas Santander, James Bryden Isbister, Ioannis Magkanaris, Sirio Bolaños Puchet, Jorge Blanco Alonso

Synaptic plasticity underlies the brain’s ability to learn and adapt. While experiments in brain slices have revealed mechanisms and protocols for the induction of plasticity between pairs of neurons, how these synaptic changes are coordinated in biologica ...

2024

Principles of Network Plasticity in Neocortical Microcircuits

András Ecker

Synaptic plasticity underlies our ability to learn and adapt to the constantly changing environment. The phenomenon of synapses changing their efficacy in an activity-dependent manner is often studied in small groups of neurons in vitro or indirectly throu ...

EPFL2023

Modeling and simulation of neocortical micro- and mesocircuitry. Part II: Physiology and experimentation

Weina Ji, Henry Markram, Felix Schürmann, Daniel Keller, Eilif Benjamin Muller, Michael Reimann, Werner Alfons Hilda Van Geit, Srikanth Ramaswamy, Matthias Wolf, James Gonzalo King, Alexis Arnaudon, András Ecker, Rajnish Ranjan, Jean-Denis Georges Emile Courcol, Armando Romani, Pramod Shivaji Kumbhar, Judit Planas Carbonell, Giuseppe Chindemi, Michael Emiel Gevaert, Christian Andreas Rössert, Fernando Joaquim Leite Pereira, Omar Awile, Mustafa Anil Tuncel, Daniela Egas Santander, James Bryden Isbister, Natali Barros Zulaica, Samuel Lieven D. Lapere, Sirio Bolaños Puchet, Maria Reva, Genrich Ivaska, Tanguy Pierre Louis Damart, Darshan Mandge, Joni Henrikki Herttuainen, Christoph Pokorny, Elvis Boci, Vishal Sood, Thomas Brice Delemontex, Aleksandra Zuzanna Teska, Polina Litvak, Alexander Dietz, Jorge Blanco Alonso, Gianluca Ficarelli

Cortical dynamics underlie many cognitive processes and emerge from complex multi-scale interactions, which are challenging to study in vivo. Large-scale, biophysically detailed models offer a tool which can complement laboratory approaches. We present a m ...

2024

Drosophila SPG12 ortholog, reticulon-like 1, governs presynaptic ER organization and Ca2+ dynamics

Rebecca Carolyn Smith

Neuronal endoplasmic reticulum (ER) appears continuous throughout the cell. Its shape and continuity are influenced by ER-shaping proteins, mutations in which can cause distal axon degeneration in Hereditary Spastic Paraplegia (HSP). We therefore asked how ...

Rockefeller University Press2023

Different responses of mice and rats hippocampus CA1 pyramidal neurons to in vitro and in vivo-like inputs

Ying Shi, Armando Romani, Maurizio Ferdinando Pezzoli

The fundamental role of any neuron within a network is to transform complex spatiotemporal synaptic input patterns into individual output spikes. These spikes, in turn, act as inputs for other neurons in the network. Neurons must execute this function acro ...

2023

Mechanisms Underlying the Recruitment of Inhibitory Interneurons in Fictive Swimming in Developing Xenopus laevis Tadpoles

Andrea Ferrario

Developing spinal circuits generate patterned motor outputs while many neurons with high membrane resistances are still maturing. In the spinal cord of hatchling frog tadpoles of unknown sex, we found that the firing reliability in swimming of inhibitory i ...

SOC NEUROSCIENCE2023

Plasticity of sensory representations in the posterior insular cortex during fear learning

Denys Osypenko

Aversively-motivated associative learning allows animals to avoid harm and thus ensures survival. Aversive learning can be studied by the fear learning paradigm, in which an innocuous sensory stimulus like a tone (conditioned stimulus, CS), acquires a nega ...

EPFL2023

The matricellular protein Drosophila Cellular Communication Network Factor is required for synaptic transmission and female fertility

Evelyne Ruchti, Brian Donal McCabe

Within the extracellular matrix, matricellular proteins are dynamically expressed nonstructural proteins that interact with cell surface receptors, growth factors, and proteases, as well as with structural matrix proteins. The cellular communication networ ...

Genetics Society America2023

A conditional GABAergic synaptic vesicle marker for Drosophila

Brian Donal McCabe

Background: Throughout the animal kingdom, GABA is the principal inhibitory neurotransmitter of the nervous system. It is essential for maintaining the homeostatic balance between excitation and inhibition required for the brain to operate normally. Identi ...

2022

Modulation of working memory duration by synaptic and astrocytic mechanisms

Sophia Becker

Short-term synaptic plasticity and modulations of the presynaptic vesicle release rate are key components of many working memory models. At the same time, an increasing number of studies suggests a potential role of astrocytes in modulating higher cognitiv ...

PUBLIC LIBRARY SCIENCE2022

A conditional glutamatergic synaptic vesicle marker for Drosophila

Evelyne Ruchti, Brian Donal McCabe

Glutamate is a principal neurotransmitter used extensively by the nervous systems of all vertebrate and invertebrate animals. It is primarily an excitatory neurotransmitter that has been implicated in nervous system development, as well as a myriad of brai ...

OXFORD UNIV PRESS INC2022

Fear Learning: An Evolving Picture for Plasticity at Synaptic Afferents to the Amygdala

Ralf Schneggenburger, Denys Osypenko, Shriya Palchaudhuri

Unraveling the neuronal mechanisms of fear learning might allow neuroscientists to make links between a learned behavior and the underlying plasticity at specific synaptic connections. In fear learning, an innocuous sensory event such as a tone (called the ...

SAGE PUBLICATIONS INC2022

The functional characterization of callosal connections

Muhamed Barakovic, Giorgio Innocenti

The brain operates through the synaptic interaction of distant neurons within flexible, often heterogeneous, distributed systems. Histological studies have detailed the connections between distant neurons, but their functional characterization deserves fur ...

PERGAMON-ELSEVIER SCIENCE LTD2022

Mesoscopic description of hippocampal replay and metastability in spiking neural networks with short-term plasticity

Tilo Schwalger, Valentin Marc Schmutz

Bottom-up models of functionally relevant patterns of neural activity provide an explicit link between neuronal dynamics and computation. A prime example of functional activity patterns are propagating bursts of place-cell activities called hippocampal rep ...

PUBLIC LIBRARY SCIENCE2022

Pathological substrate of memory impairment in multiple system atrophy

Hilal Lashuel, Melek Firat Altay

Aims: Synaptic dysfunction in Parkinson's disease is caused by propagation of pathogenic alpha-synuclein between neurons. Previously, in multiple system atrophy (MSA), pathologically characterised by ectopic deposition of abnormal alpha-synuclein predomina ...

WILEY2022

A subpopulation of cortical VIP-expressing interneurons with highly dynamic spines

Graham Knott, Jérôme Blanc

Structural synaptic plasticity may underlie experience and learning-dependent changes in cortical circuits. In contrast to excitatory pyramidal neurons, insight into the structural plasticity of inhibitory neurons remains limited. Interneurons are divided ...

NATURE PORTFOLIO2022

Homeostatic regulation of axonal Kv1.1 channels accounts for both synaptic and intrinsic modifications in the hippocampal CA3 circuit

Mickael Maurice Zbili

Homeostatic plasticity of intrinsic excitability goes hand in hand with homeostatic plasticity of synaptic transmission. However, the mechanisms linking the two forms of homeostatic regulation have not been identified so far. Using electrophysiological, im ...

National Academy of Sciences2021

Wnt5a promotes hippocampal postsynaptic development and GluN2B-induced expression via the eIF2 alpha HRI kinase

Eva Ramos Fernandez

Wnt signaling plays a key role in neurodevelopment and neuronal maturation. Specifically, Wnt5a stimulates postsynaptic assemblies, increases glutamatergic neurotransmission and, through calcium signaling, generates nitric oxide (NO). Trying to unveil the ...

Nature Research2021

Modelling and Refining Neuronal Circuits with Guidance Cues: Involvement of Semaphorins

Greta Limoni

The establishment of neuronal circuits requires neurons to develop and maintain appropriate connections with cellular partners in and out the central nervous system. These phenomena include elaboration of dendritic arborization and formation of synaptic co ...

MDPI2021