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The invention relates to the field of in vitro detection methods using luminescence. Provided is a sensor molecule for detecting an analyte of interest in a sample using bioluminescence resonance energy transfer (BRET), the sensor molecule comprising a proteinaceous moiety tethered to a synthetic regulatory molecule. Also provided is an analytical device comprising a sensor and methods using the sensor molecule.
Ardemis Anoush Boghossian, Alice Judith Gillen
Helen Rebecca Ostlund Farrants
Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü