Publication

Means and methods for bioluminescence resonance energy transfer (bret) analysis in a biological sample

Related publications (13)

Non-covalent Methods of Engineering Optical Sensors Based on Single-Walled Carbon Nanotubes

Ardemis Anoush Boghossian, Alice Judith Gillen

Optical sensors based on single-walled carbon nanotubes (SWCNTs) demonstrate tradeoffs that limit their use in in vivo and in vitro environments. Sensor characteristics are primarily governed by the non-covalent wrapping used to suspend the hydrophobic SWC ...

2019

Allosteric Protein Switches based on Antibody Mimetics

Helen Rebecca Ostlund Farrants

Synthetic protein switches are proteins that can be controlled by an external input, and are useful tools to probe protein function. Antibody fragments and antibody mimetics can be selected to bind, activate, or inhibit several diverse protein targets. Nev ...

EPFL2019

A time-gated large-array SPAD camera for picosecond resolution real-time FLIM

Claudio Bruschini, Edoardo Charbon, Arin Can Ülkü

Single-photon avalanche diode (SPAD) arrays have recently emerged as promising detectors for many wide-field fluorescence lifetime imaging microscopy (FLIM) applications, thanks to their picosecond range temporal resolution, single-photon sensitivity and f ...

2018

Applications of a reconfigurable SPAD line imager

Claudio Bruschini, Edoardo Charbon, Samuel Burri

Single-photon avalanche diodes (SPADs) fabricated in standard CMOS processes enable cost-effective volume production of sensors capable of time-stamping individual photons with high accuracy. This capability makes the sensors ideally suited for application ...

2018

Bioluminescent Antibodies for Point-of-Care Diagnostics

Kai Johnsson, Alberto Schena, Rudolf Griss, Qiuliyang Yu, Lin Xue

We introduce a general method to transform antibodies into ratiometric, bioluminescent sensor proteins for the no-wash quantification of analytes. Our approach is based on the genetic fusion of antibody fragments to NanoLuc luciferase and SNAP-tag, the lat ...

Wiley-VCH Verlag GmbH2017

Fluorescence lifetime imaging using a single photon avalanche diode array sensor

Claudio Bruschini, Edoardo Charbon, Samuel Burri, Ivan Michel Antolovic

Single photon detectors allows us work with the weakest fluorescence signals. Single photon arrays, combined with ps-controlled gating allow us to create image maps of fluorescence lifetimes, which can be used for in-vivo discrimination of tissue activity. ...

2017

LinoSPAD: a time-resolved 256x1 CMOS SPAD line sensor system featuring 64 FPGA-based TDC channels running at up to 8.5 giga-events per second

Claudio Bruschini, Edoardo Charbon, Samuel Burri, Harald Arjan Robert Homulle

LinoSPAD is a reconfigurable camera sensor with a 256x 1 CMOS SPAD (single-photon avalanche diode) pixel array connected to a low cost Xilinx Spartan 6 FPGA. The LinoSPAD sensor's line of pixels has a pitch of 24 pm and 40% fill factor. The FPGA implements ...

Spie-Int Soc Optical Engineering2016

Sensor molecules and uses thereof

Kai Johnsson, Alberto Schena, Rudolf Griss

The invention generally relates to methods, reagents and devices for determining a concentration of an analyte in a sample. Provided is a proteinaceous sensor molecule for detecting an analyte of interest (A), comprising a signal generating moiety tethered ...

2015

Novel Chemosensors for Biologically Important Analytes

Ziya Köstereli

In this work, fluorescent-based chemosensors for the detection of important analytes in aqueous solution are described. The sensors are based on different detection concepts, including analyte-induced aggregation/de-aggregation, pattern-based sensing and m ...

EPFL2015

Architecture and applications of a high resolution gated SPAD image sensor

Claudio Bruschini, Edoardo Charbon, Samuel Burri, Francesco Regazzoni

We present the architecture and three applications of the largest resolution image sensor based on single-photon avalanche diodes (SPADs) published to date. The sensor, fabricated in a high-voltage CMOS process, has a resolution of 512 x 128 pixels and a p ...

Optical Soc Amer2014

A 65k pixel, 150k frames-per-second camera with global gating and micro-lenses suitable for fluorescence lifetime imaging

Claudio Bruschini, Edoardo Charbon, Samuel Burri, Francesco Regazzoni, François Eric Powolny

This paper presents our work on a 65k pixel single-photon avalanche diode (SPAD) based imaging sensor realized in a 0.35 mu m standard CMOS process. At a resolution of 512 by 128 pixels the sensor is read out in 6.4ps to deliver over 150k monochrome frames ...

Spie-Int Soc Optical Engineering2014

Metal-Based Chemosensors for Important Bioanalytes

Sébastien Rochat

This work describes different strategies for the detection of biologically relevant analytes. The main goal was to achieve sensing in water, which is a prerequisite for application to real biological samples. In several cases, we could demonstrate that our ...

EPFL2010

A Single Photon Avalanche Diode Array Fabricated in 0.35um CMOS and based on an Event-Driven Readout for TCSPC Experiments

Cristiano Niclass, Edoardo Charbon

The design and characterization of an imaging sensor based on single photon avalanche diodes is presented. The sensor was fully integrated in a 0.35µm CMOS technology. The core of the imager is an array of 4x112 pixels that independently and simultaneously ...

2006