Publication

DNA extraction from soil samples: Time for high throughput efficacy

Luiz Felippe De Alencastro
2011
Posters

Abstract

Total DNA extraction is the key stage allowing the implementation of various downstream applications in molecular ecology. The widespread application of downstream technologies has led to an increasing demand for highly pure biomolecules. Manual methods and proprietary extraction kits used for the purification of nucleic acids can be laborious and time‐consuming. The breadth of sample types encountered and the increasing number of samples processed result in seeking new ways to enhance sample preparation efficiency and reliability. Representativeness, sensitivity and reproducibility are essential requirements for such an extraction technique that should be applied in a high throughput mode.

Official source

https://infoscience.epfl.ch/entities/publication/ac3ad9af-a473-4927-8ce6-3bbba0bfa53f

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Luiz Felippe De Alencastro
2011
Posters

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/ac3ad9af-a473-4927-8ce6-3bbba0bfa53f

About this result

Related concepts (32)

High-throughput screening

High-throughput screening (HTS) is a method for scientific experimentation especially used in drug discovery and relevant to the fields of biology, materials science and chemistry. Using robotics, data processing/control software, liquid handling devices, and sensitive detectors, high-throughput screening allows a researcher to quickly conduct millions of chemical, genetic, or pharmacological tests. Through this process one can quickly recognize active compounds, antibodies, or genes that modulate a particular biomolecular pathway.

DNA extraction

The first isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher. DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue . It involves breaking open the cells, removing proteins and other contaminants, and purifying the DNA so that it is free of other cellular components. The purified DNA can then be used for downstream applications such as PCR, sequencing, or cloning.

DNA sequencing

DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery. Knowledge of DNA sequences has become indispensable for basic biological research, DNA Genographic Projects and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics.

Related publications (21)

DNA extraction from soil samples: Time for high throughput efficacy

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