Reduced Dyes Enhance Single-Molecule Localization Density for Live Superresolution Imaging

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Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry

Demetri Psaltis, Joowon Lim, Daniele Pirone

The accurate identification of the three-dimensional quantitative shape of a cell nucleus is now possible without fluorescent staining by applying computational segmentation to refractive index tomograms recorded in the flow cytometry mode. ...

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The topic of this thesis is the development of new algorithmic reconstruction methods for quantitative phase imaging (QPI). In the past decade, advanced QPI has emerged as a valuable tool to study label-free biological samples and uncover their 3D structur ...

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Event-driven acquisition for content-enriched microscopy

Suliana Manley, Dora Mahecic, Juliette Griffie, Chen Zhang, Martin Weigert, Willi Leopold Stepp

Event-driven acquisition uses neural-network-based recognition of specific biological events to trigger switching between slow and fast super-resolution imaging, enriching the capture of interesting events with high spatiotemporal resolution. A common goal ...

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Fluorescent D-amino acids (FDAAs) have previ-ously been developed to enable in situ highlighting of locations of bacterial cell wall growth. Most bacterial cells lie at the edge of the diffraction limit of visible light; thus, resolving the precise details ...

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Super-resolution microscopy at angstrom precision could pave the way to optical structural biology in cells. ...

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A method for operating a light microscope comprises emitting and guiding a plurality of illumination light beams towards a specimen (6) to form a plurality of separated illumination light spots (2A, 2B, 2C, 2D) at the specimen; and guiding detection light ...

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The search for an understanding of the causal elements that lead to neurodegenerative diseases has motivated researchers for decades. Today, Alzheimer's disease is the most prevalent form of dementia and affects approximately fifty million people worldwide ...

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Pixel super-resolution with spatially entangled photons

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Pixelation is common in quantum imaging systems and limit the image spatial resolution. Here, the authors introduce a pixel super-resolution approach based on measuring the full spatially-resolved joint probability distribution of spatially-entangled photo ...

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Bacteria are ubiquitously found in all sorts of environment. They're found in the ocean, soil, or even in our guts or on our skin. Independently of their niche, they can transition from a planktonic state were they freely swim in an aqueous environment to ...

EPFL2022

SPAD array technology enables fluctuation-contrast super-resolution in a confocal microscope

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A fluorescence correlation contrast is a straightforward approach to super-resolution imaging. Combining a SPAD array with a novel detection scheme (ISM), we obtain images with up to x4 times resolution enhancement. ...

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Single-molecule localization microscopy

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Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at the molecular scal ...

SPRINGERNATURE2021

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