Publications related to A general method for the development of multicolor biosensors with large dynamic ranges

Related publications (10)

Tracking spatiotemporal localisation and interactions of the innate immune sensor STING

Innate immunity, the very first line of defence of our cells, relies on the detection of universal pathogen- or danger-associated signals to launch an inflammatory response. A crucial part of our innate immune system is based on the recognition of out-of-c ...

EPFL2023

High sensitivity, incoherent differential imaging

Chiara Bonati

Microscopic visualisation of optically transparent samples has been a topic of interest for several decades. Features such as density or chemical composition can influence the optical phase of transmitted light, and phase contrast can reveal these structur ...

EPFL2022

Hypervalent Iodine-Mediated Late-Stage Peptide and Protein Functionalization

Jérôme Waser, Elija Grinhagena, Emmanuelle Madeline Dominique Allouche

Hypervalent iodine compounds are powerful reagents for the development of novel transformations. As they exhibit low toxicity, high functional group tolerance, and stability in biocompatible media, they have been used for the functionalization of biomolecu ...

Wiley-VCH Verlag GmbH2022

Ultrasensitive Label-Free Detection of Protein–Membrane Interaction Exemplified by Toxin-Liposome Insertion

Sylvie Roke, Matteo Dal Peraro, Halil Ibrahim Okur, Chan Cao, Mircea Ioan Iacovache, Tereza Schönfeldová

Measuring the high-affinity binding of proteins to liposome membranes remains a challenge. Here, we show an ultrasensitive and direct detection of protein binding to liposome membranes using high throughput second harmonic scattering (SHS). Perfringolysin ...

2022

Integration of polymeric membrane/dielectric sphere assemblies in microfluidic chips for enhanced-contrast imaging with low-magnification systems

Martinus Adela Maria Gijs, Daniel Migliozzi, Vittorio Viri

Current microscopy systems for the imaging of microorganisms are expensive because of their optimized design toward resolution maximization and aberration correction. In situations where such an optimization is not needed, for instance to merely detect the ...

SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS2021

Computation and Visualization in Multiscale Modelling of DNA Mechanics

Jaroslaw Glowacki

Amongst the substances found in any living organism one of the most central in the workings of each living cell is deoxyribonucleic acid (DNA). For that reason it has been the subject of research in many of its aspects. These range from DNA sequencing to a ...

EPFL2016

On the Impact of Integration of Non-Image Forming (NIF) Effect of Light on Electrical Lighting Control in Non-Residential Buildings

Jean-Louis Scartezzini, Ali Motamed, Marta Benedetti

Lighting has an impact on the visual as well as cognitive performance of people in non-residential buildings. Light is the most powerful time cue for resetting the circadian pacemaker and ensuring correct synchronization of the internal clock with the envi ...

2016

Microfluidic chip for monitoring Ca2+ transport through a confluent layer of intestinal cells

Martinus Adela Maria Gijs, Paolo Silacci, Hüseyin Cumhur Tekin, Josias Basil Wacker

The absorption of dietary calcium through the intestinal barrier is essential for maintaining health in general and especially of the bone system. We propose a microfluidic model that studies free calcium (Ca2+) transport through a confluent monolayer of C ...

Royal Soc Chemistry2014

Cell permeable, fluorescent dye

Kai Johnsson, Grazvydas Lukinavicius

The invention pertains to a near-infrared fluorescent dye that is cell permeable and can be attached to selected proteins in living cells. The dye has the general formula (I) or its or its corresponding spirolactone (II) wherein Y is chosen from the group ...

2013

Harmonic Nanocrystals for Bio-Labeling: a Survey of Optical Properties and Biocompatibility

Sandrine Gerber, Luigi Bonacina, Solène Marcelle Françoise Marie Passemard, Jérôme Extermann, Davide Städler

Nonlinear optical nanocrystals have been recently introduced as a promising alternative to fluorescent probes for multi-photon microscopy. We present for the first time a complete survey of the properties of the different nanomaterials proposed to date, de ...

American Chemical Society2012