Publication

Single molecule detection at surfaces

Theo Lasser, Marcel Leutenegger
2007
Book Chapters

Abstract

Fluorescence fluctuation spectroscopy based on an evanescent field excitation scheme leads to an alternative concept for single molecule detection with interesting experimental features. This concept is described and analyzed regarding the total fluorescence process including excitation, fluorescence emission and collection close to a dielectric interface. The realized experimental scheme showed superior performance of this concept with substantially enhanced molecular brightness when compared to the classical confocal setup based on water immersion objectives. Selected applications in life sciences including dual-color surface fluorescence correlation spectroscopy, enzyme kinetics and receptor--ligand binding are underlining the interest of this experimental approach.

Official source

https://infoscience.epfl.ch/entities/publication/82055d1b-d488-47c1-8497-7e0dcc46b803

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Theo Lasser, Marcel Leutenegger
2007
Book Chapters

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/82055d1b-d488-47c1-8497-7e0dcc46b803

About this result

Related concepts (32)

Fluorescence microscope

A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances. "Fluorescence microscope" refers to any microscope that uses fluorescence to generate an image, whether it is a simple set up like an epifluorescence microscope or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescence image.

Fluorescence

Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore a lower photon energy, than the absorbed radiation. A perceptible example of fluorescence occurs when the absorbed radiation is in the ultraviolet region of the electromagnetic spectrum (invisible to the human eye), while the emitted light is in the visible region; this gives the fluorescent substance a distinct color that can only be seen when the substance has been exposed to UV light.

Super-resolution microscopy

Super-resolution microscopy is a series of techniques in optical microscopy that allow such images to have resolutions higher than those imposed by the diffraction limit, which is due to the diffraction of light. Super-resolution imaging techniques rely on the near-field (photon-tunneling microscopy as well as those that use the Pendry Superlens and near field scanning optical microscopy) or on the far-field.

Related publications (4)

Single molecule detection at surfaces

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Detection of early bladder carcinoma by fluorescence cystoscopy with Hexvix(R): Improvement of the specificity by microcystoscopy

Parallel Confocal Detection of Single Biomolecules using Diffractive Optics and Integrated Detector Units