Publications related to Exploiting Covalent Chemical Labeling with Self-Labeling Proteins

Related publications (20)

Bioanode engineering for living solar cells

Whole-cell biophotovoltaic (BPV) devices leverage photoautotrophic microorganisms like cyanobacteria to convert sunlight and water into bioelectricity. They are capable of growing on sequestered carbon dioxide (CO2), eliminating the need to add exogenous o ...

EPFL2025

Revealing chromatin-specific functions of histone deacylases

Beat Fierz, Carlos Moreno Yruela

Histone deacylases are erasers of Nε-acyl-lysine post-translational modifications and have been targeted for decades for the treatment of cancer, neurodegeneration and other disorders. Due to their relatively promiscuous activity on peptide substrates in v ...

2024

Light- field tomographic fluorescence lifetime imaging microscopy

Edoardo Charbon, Claudio Bruschini, Samuel Burri

Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging technique that enables the visualization of biological samples at the molecular level by measuring the fluorescence decay rate of fluorescent probes. This provides critical information a ...

National Academy of Sciences2024

Hard x-ray grazing-incidence ptychography: large field-of-view nanostructure imaging with ultra-high surface sensitivity

Manuel Guizar Sicairos

The morphology and distribution of nanoscale structures, such as catalytic active nanoparticles and quantum dots on surfaces, have a significant impact on their function. Thus, the capability of monitoring these properties during manufacturing and operatio ...

Optica Publishing Group2024

Time-Resolved Scanning Ion Conductance Microscopy and Single-Molecule Spectroscopy

Samuel Mendes Leitão

The evolution of the 3D morphology is at the center of many relevant biological processes ranging from cellular differentiation to cancer invasion and metastasis. Microscopy techniques, such as electron microscopy (EM), super-resolution (SR) optical micros ...

EPFL2023

Mitochondrial nanomotion measured by optical microscopy

Sandor Kasas, María Inés Villalba

Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and de ...

Frontiers Media SA2023

Optical imaging of the small intestine immune compartment across scales

Aleksandra Radenovic, Olivier Burri, Arielle Louise Planchette, Alessio Mylonas

The limitations of 2D microscopy constrain our ability to observe and understand tissue-wide networks that are, by nature, 3-dimensional. Optical projection tomography (OPT) enables the acquisition of large volumes (ranging from micrometres to centimetres) ...

NATURE PORTFOLIO2023

Nfinder: automatic inference of cell neighborhood in 2D and 3D using nuclear markers

Santiago Nicolás Rodriguez Alvarez

BackgroundIn tissues and organisms, the coordination of neighboring cells is essential to maintain their properties and functions. Therefore, knowing which cells are adjacent is crucial to understand biological processes that involve physical interactions ...

2023

BigNeuron: a resource to benchmark and predict performance of algorithms for automated tracing of neurons in light microscopy datasets

Pascal Fua, Sean Lewis Hill, Jiancheng Yang, Amos Sironi, Jian Yang

BigNeuron is an open community bench-testing platform with the goal of setting open standards for accurate and fast automatic neuron tracing. We gathered a diverse set of image volumes across several species that is representative of the data obtained in m ...

Nature Publishing Group2023

Cross-resolution Face Recognition via Identity-Preserving Network and Knowledge Distillation

Touradj Ebrahimi, Yuhang Lu

Cross-resolution face recognition has become a challenging problem for modern deep face recognition systems. It aims at matching a low-resolution probe image with high-resolution gallery images registered in a database. Existing methods mainly leverage pri ...

2023

Effectiveness of X-ray micro-CT applications upon mafic and ultramafic ophiolitic rocks

Pascal Turberg

X-ray micro-computed tomography (mu CT) was applied upon selected ophiolitic rock samples from various localities of the Vardar ophiolite outcrops in North Greece. Effectiveness of the mu CT application was evaluated through this case study by comparing re ...

2022

Towards automating de novo protein design for novel functionalities: controlling protein folds and protein-protein interactions

Zander Harteveld

The sheer size of the protein sequence space is massive: a protein of 100 residues can have 20^100 possible sequence combinations; and knowing that this exceeds the number of atoms in the universe, the chance of randomly discovering a stable new sequence w ...

EPFL2022

3D Structure From 2D Microscopy Images Using Deep Learning

Suliana Manley, Christian Sieben

Understanding the structure of a protein complex is crucial in determining its function. However, retrieving accurate 3D structures from microscopy images is highly challenging, particularly as many imaging modalities are two-dimensional. Recent advances i ...

Frontiers Media Sa2021

Experimental Combination of Super-Resolution Optical Fluctuation Imaging with Structured Illumination Microscopy for Large Fields-of-View

Aleksandra Radenovic, Suliana Manley, Vytautas Navikas, Adrien Charles-François Raymond Descloux, Dora Mahecic, Kristin Stefanie Grussmayer

All fluorescence super-resolution microscopy techniques present trade-offs between, for example, resolution, acquisition speed, and live-cell compatibility. Structured illumination microscopy (SIM) improves the resolution through successive imaging of the ...

AMER CHEMICAL SOC2021

A constrained method for lensless coherent imaging of thin samples

Michaël Unser, Christophe Moser, Manon Rostykus

Lensless inline holography can produce high-resolution images over a large field of view (FoV). In a previous work [Appl. Opt.60, B38 (2021)], we showed that (i) the actual FoV can be extrapolated outside of the camera FoV and (ii) the effective resolution ...

2021

25th Anniversary of STED Microscopy and the 20th Anniversary of SIM: feature introduction

Suliana Manley

This feature issue commemorating 25 years of STED microscopy and 20 years of SIM is intended to highlight the incredible progress and growth in the field of superresolution microscopy since Stefan Hell and Jan Wichmann published the article Breaking the di ...

2020

Self-Blinking Dyes Unlock High-Order and Multiplane Super-Resolution Optical Fluctuation Imaging

Theo Lasser, Aleksandra Radenovic, Tomas Lukes, Kristin Stefanie Grussmayer

Most diffraction-unlimited super-resolution imaging critically depends on the switching of fluorophores between at least two states, often induced using intense laser light and specialized buffers or UV radiation. Recently, so-called self-blinking dyes tha ...

AMER CHEMICAL SOC2020

Homogeneous multifocal excitation for high-throughput super-resolution imaging

Davide Gambarotto, Pierre Gönczy, Suliana Manley, Paul Guichard, Dora Mahecic, Kyle Michael Douglass, Niccolo Banterle, Virginie Hamel, Khalid Ashraf Mohie Ibrahim

Super-resolution microscopies have become an established tool in biological research. However, imaging throughput remains a main bottleneck in acquiring large datasets required for quantitative biology. Here we describe multifocal flat illumination for fie ...

Nature Publishing Group2020

The sliding Frank-Wolfe algorithm and its application to super-resolution microscopy

Emmanuel Emilien Louis Soubies, Quentin Alain Denoyelle

This paper showcases the theoretical and numerical performance of the Sliding Frank-Wolfe, which is a novel optimization algorithm to solve the BLASSO sparse spikes super-resolution problem. The BLASSO is a continuous (i.e. off-the-grid or grid-less) count ...

2020

Single-Photon, Time-Gated, Phasor-Based Fluorescence Lifetime Imaging through Highly Scattering Medium

Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü

Fluorescence lifetime imaging (FLI) is increasingly recognized as a powerful tool for biochemical and cellular investigations, including in vivo applications. Fluorescence lifetime is an intrinsic characteristic of any fluorescent dye which, to a large ext ...

AMER CHEMICAL SOC2020