Publication

Highly electrically insulating tethered lipid bilayers for probing the function of Ion channel proteins

Horst Arthur Vogel, Samuel Terrettaz, Michael Mayer
2003
Journal Articles

Abstract

A method is presented to form gold-electrode-tethered lipid bilayers with exceptionally high elec. resistances. Elec. impedance spectroscopy is used to monitor the bilayer incorporation of a ligand-gated ion channel protein and the modulation of its channel activity by the selective binding of an antibody. Due to the low defect d. of the tethered membrane, the effect of a few channels can be resolved thus opening the way to single-channel expts. on this highly stable and versatile platform. In turn, a minute quantity of analyte, here antibodies, can be measured, which is of great interest for bioanalytics. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/44f58254-c445-4014-b2ae-6053450ebd58

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Horst Arthur Vogel, Samuel Terrettaz, Michael Mayer
2003
Journal Articles

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/44f58254-c445-4014-b2ae-6053450ebd58

About this result

Related concepts (22)

Ion channel

Ion channels are pore-forming membrane proteins that allow ions to pass through the channel pore. Their functions include establishing a resting membrane potential, shaping action potentials and other electrical signals by gating the flow of ions across the cell membrane, controlling the flow of ions across secretory and epithelial cells, and regulating cell volume. Ion channels are present in the membranes of all cells. Ion channels are one of the two classes of ionophoric proteins, the other being ion transporters.

Ligand-gated ion channel

Ligand-gated ion channels (LICs, LGIC), also commonly referred to as ionotropic receptors, are a group of transmembrane ion-channel proteins which open to allow ions such as Na+, K+, Ca2+, and/or Cl− to pass through the membrane in response to the binding of a chemical messenger (i.e. a ligand), such as a neurotransmitter. When a presynaptic neuron is excited, it releases a neurotransmitter from vesicles into the synaptic cleft. The neurotransmitter then binds to receptors located on the postsynaptic neuron.

Sodium channel

Sodium channels are integral membrane proteins that form ion channels, conducting sodium ions (Na+) through a cell's membrane. They belong to the superfamily of cation channels. They are classified into 2 types: In excitable cells such as neurons, myocytes, and certain types of glia, sodium channels are responsible for the rising phase of action potentials. These channels go through three different states called resting, active and inactive states.

Related publications (10)

Highly electrically insulating tethered lipid bilayers for probing the function of Ion channel proteins

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