Publication

Characterization of a transmembrane receptor protein by fluorescence correlation spectroscopy

Horst Arthur Vogel, Rudolf Hovius, Horst Pick
2001
Conference Papers

Abstract

Fluorescence Correlation Spectroscopy (FCS) is a suitable technique to investigate biomol. interactions. The advantage of FCS lies in its wide dynamic range in the time and concn. domain, its sensitivity, and thus in the small amt. of material needed. The characteristic time of the processes that can be measured spans more than eleven orders of magnitude, between several nanoseconds up to hours; the measurable concn. range extends from 0.1 nM to several mM. Here we report on FCS expts. to investigate the properties of the 5-hydroxytryptamine (=serotonin) receptor of type 3 (5HT3-R) in vitro and in vivo. The 5HT3-R is a homopentamer, consisting of subunits of a relative mol. mass of 54 kDa. This neuroreceptor functions as a ligand-gated ion channel, which influences among other things anxiety and depression in human beings. It is therefore a good example for a pharmacol. important membrane receptor. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/31528827-177d-4c9e-afd2-c813b2080873

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Horst Arthur Vogel, Rudolf Hovius, Horst Pick
2001
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/31528827-177d-4c9e-afd2-c813b2080873

About this result

Related concepts (32)

G protein-coupled receptor

G protein-coupled receptors (GPCRs), also known as seven-(pass)-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptors, and G protein-linked receptors (GPLR), form a large group of evolutionarily related proteins that are cell surface receptors that detect molecules outside the cell and activate cellular responses. They are coupled with G proteins.

Cell surface receptor

Cell surface receptors (membrane receptors, transmembrane receptors) are receptors that are embedded in the plasma membrane of cells. They act in cell signaling by receiving (binding to) extracellular molecules. They are specialized integral membrane proteins that allow communication between the cell and the extracellular space. The extracellular molecules may be hormones, neurotransmitters, cytokines, growth factors, cell adhesion molecules, or nutrients; they react with the receptor to induce changes in the metabolism and activity of a cell.

Receptor (biochemistry)

In biochemistry and pharmacology, receptors are chemical structures, composed of protein, that receive and transduce signals that may be integrated into biological systems. These signals are typically chemical messengers which bind to a receptor and produce physiological responses such as change in the electrical activity of a cell. For example, GABA, an inhibitory neurotransmitter inhibits electrical activity of neurons by binding to GABA_A receptors.

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Characterization of a transmembrane receptor protein by fluorescence correlation spectroscopy

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