Open-source microscope add-on for structured illumination microscopy

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Developing bones can be severely impaired by a range of disorders where muscular loading is abnormal. Recent work has indicated that the effects of absent skeletal muscle on bones are more severe early in development, with rudiment length and mineralizatio ...

Super-resolution structured-illumination microscopy (SIM) is a powerful technique that allows one to surpass the diffraction limit by up to a factor two. Yet, its practical use is hampered by its sensitivity to imaging conditions which makes it prone to re ...

The community-driven initiative Quality Assessment and Reproducibility for Instruments & Images in Light Microscopy (QUAREP-LiMi) wants to improve reproducibility for light microscopy image data through quality control (QC) management of instruments and im ...

We introduce a new generation of 3D imaging devices based on quantum plenoptic imaging. Position-momentum entanglement and photon number correlations are exploited to provide a scan-free 3D image after post-processing of the collected light intensity signa ...

Mycobacterium tuberculosis, the etiological agent for the tuberculosis disease, is a bacterial pathogen thought to infect about a quarter of the global human population. It is the first cause of death among infectious diseases, and is most prevalent in low ...

The design of highly glazed building has become a worldwide trend in modern architecture. However, glazing may induce large thermal loss in winter while increase cooling load in summer, and it may also cause glare. In order to solve the problems, a novel m ...

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going beyond the diffraction barrier comes at a price since most far-field super-resolution imaging techniques trade temporal for spa ...

Damage recovery after low-velocity impact has been assessed in woven E-glass fibre-reinforced polymer composites with an epoxy matrix and a blend of epoxy and 25 vol% of poly(e-caprolactone) (PCL). Impact was carried out at three energy levels (8.5, 17, 34 ...

In the last 30 years, superresolution in optical microscopy has been a major field of research. During this time, different techniques have been created to break the diffraction limit in order to make observations at a nanometric scale. Given that optical ...

While CMOS single-photon avalanche diode (SPAD) technology has steadily advanced, improving noise, timing resolution, and sensitivity, spatial resolution has been increasing as well. The increase in the number of pixels has made a comprehensive analysis of ...

Biological processes are inherently multi-scale, and supramolecular complexes at the nanoscale determine changes at the cellular scale and beyond. Single-molecule localizationmicroscopy (SMLM)(1-3) techniques have been established as important tools for st ...

Excitatory synaptic transmission is accompanied by a local surge in interstitial lactate that occurs despite adequate oxygen availability, a puzzling phenomenon termed aerobic glycolysis. In addition to its role as an energy substrate, recent studies have ...

We propose and experimentally demonstrate a digital holographic camera which can be attached to the camera port of a conventional microscope for obtaining digital holograms in a self-reference configuration, under short coherence illumination and in a sing ...

Nucleic acid synthesis is spatially organized in many organisms. In bacteria, however, the spatial distribution of transcription remains obscure, owing largely to the diffraction limit of conventional light microscopy (200-300 nm). Here, we use photoactiva ...

The ability to observe axons and dendrites with transmission electron microscopy (EM) after they have been previously imaged live with laser-scanning microscopy is a useful technique to study their synaptic connectivity. This protocol provides a detailed m ...

We introduce two methods for quantifying and evaluating the amount of surface receptors within a group of cells from fluorescence microscope images. First, the average fluorescence intensity method (AFIM), based on the fluores- cent pixels average intensit ...

Functional imaging of biological cells and organs is one of the key techniques that drives new discoveries in life science research and medicine. In this thesis novel concepts for functional imaging at high spatial and temporal resolution are presented. In ...

Multiphoton excited luminescent properties of water-soluble EuIII and TbIII complexes with derivatives of dipicolinic acid functionalized with a polyoxyethylene pendant arm and terminal groups, [Eu(LOMe)3]3-, [Eu(LNH2)3]3-, and [Tb(LOH)3]3-, as well as of ...

Fluorescence localization microscopy (i.e., PALM, STORM) has enabled optical imaging at nanometer-scale resolutions. The localization algorithms used in these techniques rely on fitting a 2-D Gaussian to the in-focus image of individual fluorophores. For f ...

A new method for the covalent and specific labeling of fusion proteins of carrier proteins (CPs) with small organic molecules has been developed in this work. This technology combines the convenience of expressing genetically tagged reporter proteins with ...

Open-source microscope add-on for structured illumination microscopy

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Lack of embryonic skeletal muscle in mice leads to abnormal mineral deposition and growth

Surpassing light inhomogeneities in structured-illumination microscopy with FlexSIM

QUAREP-LiMi: a community endeavor to advance quality assessment and reproducibility in light microscopy

Towards quantum 3D imaging devices

Time-lapse high-resolution microscopy to study the morphogenesis of microorganisms

Novel daylighting system based on advanced embedded optical microstructures for various facade orientation and climates

Combined Multi-Plane Tomographic Phase Retrieval and Stochastic Optical Fluctuation Imaging for 4D Cell Microscopy

Damage recovery after impact in E-glass reinforced poly(epsilon-caprolactone)/epoxy blends

Nanoscopy in nonlinear scanning fluorescence imaging systems

Nonuniformity Analysis of a 65-kpixel CMOS SPAD Imager

Super-resolution imaging of multiple cells by optimized flat-field epi-illumination

Channel-Mediated Lactate Release by K+-Stimulated Astrocytes

Off-axis digital holographic camera for quantitative phase microscopy

Multiscale Spatial Organization of RNA Polymerase in Escherichia coli

Imaging green fluorescent protein-labeled neurons using light and electron microscopy

Quantification of Sub-resolution Sized Targets in Cell Fluorescent Imaging

Novel Concepts for Functional High Resolution Microscopy

Multiphoton-Excited Luminescent Lanthanide Bioprobes: Two- and Three-Photon Cross Sections of Dipicolinate Derivatives and Binuclear Helicates

Steerable filters for orientation estimation and localization of fluorescent dipoles

A new method for protein labeling with small molecules based on acyl carrier protein