Publication

Membrane receptor proteins at interfaces for bioanalytics in the micro- and nanometer range

Horst Arthur Vogel
2001
Conference Papers

Abstract

A review. Cell-based assays are the predominant means of assessing activation of ionotropic receptors and G protein-coupled receptors (GPCRs), but are limited by their inherent complexity. Here we present an approach to immobilize receptors stably and with defined orientation in microarrays on sensor surfaces. Using optical and elec. methods we were able to follow ligand binding, receptor activation and receptor deactivation. Our findings are a novel approach for functional screening of pharmacol. interesting receptor proteins in high d. format. [on SciFinder (R)]

Official source

https://infoscience.epfl.ch/entities/publication/1773b8a5-343d-44cc-b67e-af5a66a271dd

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Horst Arthur Vogel
2001
Conference Papers

Abstract

Official source

https://infoscience.epfl.ch/entities/publication/1773b8a5-343d-44cc-b67e-af5a66a271dd

About this result

Related concepts (32)

G protein-coupled receptor

G protein-coupled receptors (GPCRs), also known as seven-(pass)-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptors, and G protein-linked receptors (GPLR), form a large group of evolutionarily related proteins that are cell surface receptors that detect molecules outside the cell and activate cellular responses. They are coupled with G proteins.

Ligand-gated ion channel

Ligand-gated ion channels (LICs, LGIC), also commonly referred to as ionotropic receptors, are a group of transmembrane ion-channel proteins which open to allow ions such as Na+, K+, Ca2+, and/or Cl− to pass through the membrane in response to the binding of a chemical messenger (i.e. a ligand), such as a neurotransmitter. When a presynaptic neuron is excited, it releases a neurotransmitter from vesicles into the synaptic cleft. The neurotransmitter then binds to receptors located on the postsynaptic neuron.

Receptor (biochemistry)

In biochemistry and pharmacology, receptors are chemical structures, composed of protein, that receive and transduce signals that may be integrated into biological systems. These signals are typically chemical messengers which bind to a receptor and produce physiological responses such as change in the electrical activity of a cell. For example, GABA, an inhibitory neurotransmitter inhibits electrical activity of neurons by binding to GABA_A receptors.

Related publications (1)

Membrane receptor proteins at interfaces for bioanalytics in the micro- and nanometer range

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Probing ligand-receptor recognition in G protein-coupled receptors through biosynthetic incorporation of fluorescent amino acids at specific sites and measurement of distances by fluorescence energy transfer