Publications related to Culture of preantral follicles in poly(ethylene) glycol-based, three-dimensional hydrogel: a relationship between swelling ratio and follicular developments

Engineering hydrogel microenvironments for epithelial organoid culture

Organoids, miniature tissues generated from self-organizing stem cells within three-dimensional (3D) extracellular matrices (ECM), have opened up exciting possibilities for in vitro studies of complex physiological processes. A key factor in the success of ...

EPFL2024

Enhancing Chondrocyte Biosynthetic Activities: Harnessing the Power of Loading-Induced Evolved Temperature

Theofanis Stampoultzis

Chondrocytes within articular cartilage possess the capacity to perceive and react to signals generated by the typical load-bearing actions of daily life, such as walking or running. Articular cartilage is predominantly oriented toward compression-based fu ...

EPFL2024

Controlling the Microstructure and Composition of 3D Printed Soft Load-Bearing Hydrogels

Matteo Hirsch

Hydrogels are among the first materials expressly designed for their use in biomedicine. However, state-of-the-art applications of hydrogels are severely limited because they are typically either too soft or too brittle such that they cannot bear significa ...

EPFL2023

Hybrid hydrogels for load-bearing implants

Céline Samira Wyss

Load-bearing tissues such as articular cartilage or meniscus are not infallible. They might fail due to trauma, over-solicitations, or diseases, which could subsequently alter the mobility of the patient. Repairing these damaged tissues in a minimally inva ...

EPFL2021

Design and Development of Injectable, Tough and Intrinsically-Adhesive Hydrogels for Biomedical Applications

Peyman Karami

Despite the development of hydrogels with a wide range of mechanical properties, insufficient adhesion between these materials and biological surfaces limits their use in the biomedical applications. Most recent advancements in highly adhesive hydrogel sys ...

EPFL2021

Individual differences in the Müller-Lyer and Ponzo illusions are stable across different contexts

Michael Herzog, Aline Françoise Cretenoud, Lukasz Grzeczkowski

Vision scientists have attempted to classify visual illusions according to certain aspects, such as brightness or spatial features. For example, Piaget proposed that visual illusion magnitudes either decrease or increase with age. Subsequently, it was sugg ...

2020

Three dimensional models of tissue fibrosis

Matthias Lütolf, Kristina Schoonjans, Saba Rezakhani, Ece Yildiz, Giovanni Sorrentino

The invention provides a method for obtaining a three-dimensional (3D) cellular model of tissue fibrosis, such as liver fibrosis, comprising culturing epithelial cells in a 3D hydrogel in conditions suitable for a three-dimensional cellular cluster formati ...

2020

Inference methods for the study of interacting biological oscillators in single-cells

Colas Noé Droin

Biological oscillators are pervasive in biology, covering all aspects of life from enzyme kinetics reactions to population dynamics. Although their behaviour has been intensively studied in the last decades, the recent advances of high-throughput experimen ...

EPFL2020

Extracellular Matrix Production by Mesenchymal Stromal Cells in Hydrogels Facilitates Cell Spreading and Is Inhibited by FGF-2

Martin Ehrbar, Queralt Vallmajó Martín

In native tissues, the interaction between cells and the surrounding extracellular matrix (ECM) is reciprocal, as cells not only receive signals from the ECM but also actively remodel it through secretion of cell-derived ECM. However, very little is known ...

2020

The Ovulatory Signal Precipitates LRH-1 Transcriptional Switching Mediated by Differential Chromatin Accessibility

Kristina Schoonjans

In the ovary, follicular growth and maturation are complicated processes that involve a series of morphological and physiological changes in oocytes and somatic cells leading to ovulation and luteinization, essential processes for fertility. Given the comp ...

2019

The role of mechanical hysteresis source in mechanical and mechanobiological performances of load-bearing biomaterials

Naser Nasrollahzadeh Mamaghani

Tissue engineering is a promising approach for articular cartilage regeneration as no satisfactory treatment is actually available for cartilage, partly due to the very limited self-healing capability of this tissue. To successfully induce a cartilaginous ...

EPFL2019

Control of Dissipation Sources: A Central Aspect for Enhancing the Mechanical and Mechanobiological Performances of Hydrogels

Dominique Pioletti, Naser Nasrollahzadeh Mamaghani, Peyman Karami

Development of mechanically durable and biologically inductive hydrogels is a major challenge for load-bearing applications such as engineered cartilage. Dissipative capacity of articular cartilage is central to its functional behavior when submitted to lo ...

2019

Development of functionalized alginate-based hydrogels to reduce fibrosis in the transplantation of microencapsulated cells

François Rémi Pierre Noverraz

Nowadays, the development of cell therapy relies mainly on the advances made toward cell microencapsulation which allows to evade the need for immunosuppression during cell transplantation. Among the materials considered for cell encapsulation, hydrogels d ...

EPFL2018

Composite Double-Network Hydrogels To Improve Adhesion on Biological Surfaces

Dominique Pioletti, Pierre-Etienne Bourban, Andreas Schmocker, Azadeh Khoushabi, Christophe Moser, Céline Samira Wyss, Martin Broome, Peyman Karami

Despite the development of hydrogels with high mechanical properties, insufficient adhesion between these materials and biological surfaces significantly limits their use in the biomedical field. By controlling toughening processes, we designed a composite ...

2018

Ovary-specific depletion of the nuclear receptor Nr5a2 compromises expansion of the cumulus oophorus but not fertilization by intracytoplasmic sperm injection

Kristina Schoonjans

The orphan nuclear receptor, liver receptor homolog-1 (aka Nuclear receptor subfamily 5, Group A, Member 2 (Nr5a2)), is widely expressed in mammalian tissues, and its ovarian expression is restricted to granulosa cells of activated follicles. We employed t ...

Society for the Study of Reproduction2017

Difference in suitable mechanical properties of three-dimensional, synthetic scaffolds for self-renewing mouse embryonic stem cells of different genetic backgrounds

Jeffrey Alan Hubbell, Seung Tae Lee

We evaluated whether the genetic background of embryonic stem cells (ESCs) affects the properties suitable for three-dimensional (3D) synthetic scaffolds for cell self-renewal. Inbred R1 and hybrid B6D2F1 mouse ESC lines were cultured for 7 days in hydroge ...

Wiley2017

Fighting preterm birth with novel surgical tools and biomaterials engineering

Yannick Robert Devaud

With advances in fetal diagnosis and therapy, fetoscopy has become a good option to treat a series of life threatening diseases like twin-to-twin transfusion syndrome or severe congenital diaphragmatic hernia. The efficacy of fetoscopy for those types of d ...

EPFL2017

Three dimensional hydrogels for culturing organoids

Matthias Lütolf, Nikolche Gjorevski

The invention provides hydrogels and methods for three-dimensional (3D) culture of adult epithelial stem cells and uses thereof. ...

2017

Nano Fibrillated Cellulose Based Composite Hydrogels for an Injectable Intervertebral Disc Implant

Azadeh Khoushabi

Replacing the gel-like central part of the intervertebral disc (IVD), so-called nucleus pulposus (NP), via a minimally invasive surgery is one of the most recent approaches in the lower back pain treatments. An appropriate biomaterial for NP replacement (N ...

EPFL2016

Inference for binomial probability based on dependent Bernoulli random variables with applications to meta-analysis and group level studies

Stephan Morgenthaler

We study bias arising as a result of nonlinear transformations of random variables in random or mixed effects models and its effect on inference in group-level studies or in meta-analysis. The findings are illustrated on the example of overdispersed binomi ...

Wiley-Blackwell2016