Publications related to Correlative light and electron microscopy suggests that mutant huntingtin dysregulates the endolysosomal pathway in presymptomatic Huntington's disease

Related publications (21)

Correlative light and electron microscopy for human brain and other biological models

Cornelius Laurentius Matheus van den Heuvel, Henning Paul-Julius Stahlberg, Marta Di Fabrizio, Amanda Jennifer Lewis, Christel Genoud, Notash Shafiei, Domenic Burger, Daniel Andrea Constantin Stähli

Correlative light and electron microscopy (CLEM) combines light microscopy, for identifying a target via genetic labels, dyes, antibodies and morphological features, with electron microscopy, for analyzing high-resolution subcellular ultrastructures. Here, ...

Nature Publishing Group2025

Micromechanics reveal strain rate dependent transition between dislocation mechanisms in a dual phase high entropy alloy

Johann Michler

An equimolar NiCoFeCrGa high entropy alloy having dual-phase composition was studied, where the constituent phases exhibit distinct mechanical properties. Micropillars with various diameters were created from two differently heat treated samples, then they ...

2025

Inactivation of SARS-CoV-2 at acidic pH is driven by partial unfolding of spike

Tamar Kohn, Athanasios Nenes, Aline Laetitia Schaub, Shannon Christa David

SARS-CoV-2, the causative agent of COVID-19, is predominantly transmitted by respiratory aerosol and contaminated surfaces. Recent studies demonstrated that aerosols can become acidic, and acidification has been proposed as decontamination method. Here, we ...

Springer Science and Business Media LLC2025

An approach to characterize mechanisms of action of anti-amyloidogenic compounds in vitro and in situ

Allaeddine Ahmed Ouared, Elpida Tsika, Nadine Aït Bouziad

Amyloid aggregation is associated with neurodegenerative disease and its modulation is a focus of drug development. We developed a chemical proteomics pipeline to probe the mechanism of action of anti-amyloidogenic compounds. Our approach identifies putati ...

Springer Science and Business Media LLC2025

Live-cell imaging powered by computation

Suliana Manley

The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. The prevailing challenge in live-cell fluorescence microscopy is capturing intra-cellular dynamics while preservi ...

Nature Portfolio2024

Partial crystallization in Pd-BMG systems: From understanding structure towards influencing functionality through temperature-time series

Roland Logé

Small quantities of crystalline domains created in an amorphous matrix are seen as an active driver for enhanced properties in bulk metallic glasses (BMGs). We investigated partial crystallization and phase transformations through a series of isotherms at ...

2024

Coherently amplified ultrafast imaging using a free-electron interferometer

Fabrizio Carbone

Accessing the low-energy non-equilibrium dynamics of materials and their polaritons with simultaneous high spatial and temporal resolution has been a bold frontier of electron microscopy in recent years. One of the main challenges lies in the ability to re ...

Nature Publishing Group2024

Subwavelength imaging using a solid-immersion diffractive optical processor

Demetri Psaltis, Niyazi Ulas Dinç, Carlo Gigli

Phase imaging is widely used in biomedical imaging, sensing, and material characterization, among other fields. However, direct imaging of phase objects with subwavelength resolution remains a challenge. Here, we demonstrate subwavelength imaging of phase ...

Springernature2024

Subsurface fluorescence time-of-flight imaging using a large-format single-photon avalanche diode sensor for tumor depth assessment

Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü

Significance: Fluorescence guidance is used clinically by surgeons to visualize anatomical and/or physiological phenomena in the surgical field that are difficult or impossible to detect by the naked eye. Such phenomena include tissue perfusion or molecula ...

SPIE Society of Photo-optical Instrumentation Engineers2024

A framework for evaluating the performance of SMLM cluster analysis algorithms

Daniel Sage, Juliette Griffie

This analysis compares the performance of seven algorithms for cluster analysis of single-molecule localization microscopy data. The results provide a framework for comparing these types of methods and point users to the best tools. ...

Nature Publishing Group2023

From information theory to novel imaging techniques

Jonathan Yuelin Dong

In imaging, we retrieve information about a specimen by encoding it into a probe beam. We quantitatively analyze the Fisher information provided by phase and scattering microscopy techniques and discuss implications for microscope design. ...

Optical Society of America2023

From Nano to Macro An overview of the IEEE Bio Image and Signal Processing Technical Committee

Michaël Unser, Dimitri Nestor Alice Van De Ville, Michael Stefan Daniel Liebling

The Bio Image and Signal Processing (BISP) Technical Committee (TC) of the IEEE Signal Processing Society (SPS) promotes activities within the broad technical field of biomedical image and signal processing. Areas of interest include medical and biological ...

IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC2023

Tau accumulation in degradative organelles is associated to lysosomal stress

Maurizio Molinari

Neurodegenerative disorders are characterized by the brain deposition of insoluble amyloidogenic proteins, such as alpha-synuclein or Tau, and the concomitant deterioration of cell functions such as the autophagy-lysosomal pathway (ALP). The ALP is involve ...

Nature Portfolio2023

Chemical Probe for Imaging of Polo-like Kinase 4 and Centrioles

Kai Johnsson, Pierre Gönczy, Luc Reymond, Georgios Hatzopoulos, Aleksandar Salim

Polo-like kinase (Plk4) is a serine/threonine-proteinkinase thatis essential for biogenesis of the centriole organelle and is enrichedat centrioles. Herein, we introduce Cen-TCO, a chemical probe basedon the Plk4 inhibitor centrinone, to image Plk4 and cen ...

AMER CHEMICAL SOC2023

Correlated-photon imaging at 10 volumetric images per second

Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü, Paul Mos

The correlation properties of light provide an outstanding tool to overcome the limitations of traditional imaging techniques. A relevant case is represented by correlation plenoptic imaging (CPI), a quantum-inspired volumetric imaging protocol employing s ...

Nature Portfolio2023

Deep neural network automated segmentation of cellular structures in volume electron microscopy

Martin Weigert, Benjamin Tobias Gallusser

Volume electron microscopy is an important imaging modality in contemporary cell biology. Identification of intracellular structures is a laborious process limiting the effective use of this potentially powerful tool. We resolved this bottleneck with autom ...

Rockefeller University Press2022

The new X-ray/visible microscopy MAXWELL technique for fast three-dimensional nanoimaging with isotropic resolution

Giorgio Margaritondo, Lin Yang

Microscopy by Achromatic X-rays With Emission of Laminar Light (MAXWELL) is a new X-ray/visible technique with attractive characteristics including isotropic resolution in all directions, large-volume imaging and high throughput. An ultrathin, laminar X-ra ...

Nature Portfolio2022

Off-axis electron holography for the direct visualization of perpendicular shape anisotropy in nanoscale 3D magnetic random-access-memory devices

Victor Carle Adrien Boureau

Perpendicular shape anisotropy (PSA) and double magnetic tunnel junctions (DMTJs) offer practical solutions to downscale spin-transfer-torque Magnetic Random-Access Memory (STT-MRAM) beyond 20 nm technology nodes, while retaining their thermal stability an ...

AIP Publishing2022

Analytics at the nanometer and nanosecond scales by short electron pulses in an electron microscope

Thomas La Grange

Chemical reactions and phase transformations in nanometer-size objects may happen at very short timescales, so conventional characterization tools are not applicable. A new technique combining high spatial and temporal resolution is ultrafast (or dynamic) ...

SPRINGER2022

Structure, Oxygen Content and Electric Properties of Titanium Nitride Electrodes in TiNx/La:HfO2/TiNx Stacks Grown by PEALD on SiO2/Si

Philippe Buffat, Elena Suvorova Buffat

This work reports experimental results of the quantitative determination of oxygen and band gap measurement in the TiNx electrodes in planar TiNx top/La:HfO2/TiNx bottom MIM stacks obtained by plasma enhanced atomic layer deposition on SiO2. Methodological ...

2022