Publications by Alexandre Harari

Related publications (14)

Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

George Coukos, Olaia Maria Naveiras Torres-Quiroga, Alexandre Harari, Mukul Girotra, Nicola Vannini

A fine balance of quiescence, self-renewal, and differentiation is key to preserve the hematopoietic stem cell (HSC) pool and maintain lifelong production of all mature blood cells. In recent years cellular metabolism has emerged as a crucial regulator of ...

2019

Microfluidic T Cell Selection by Cellular Avidity

Li Tang, Alexandre Harari, Armand Kurum

No T cell receptor (TCR) T cell therapies have obtained clinical approval. The lack of strategies capable of selecting and recovering potent T cell candidates may be a contributor to this. Existing protocols for selecting TCR T cell clones for cell therapi ...

WILEY2022

Tumor-associated factors are enriched in lymphatic exudate compared to plasma in metastatic melanoma patients

Melody Swartz, Romain Hamelin, Davide Demurtas, Alexandre Harari, Lambert Charles François Potin, Patricia Corthésy Henrioud

Liquid biopsies allow monitoring of cancer progression and detection of relapse, but reliable biomarkers in melanoma are lacking. Because secreted factors preferentially drain to lymphatic vessels before dilution in the blood, we hypothesized that lymph sh ...

ROCKEFELLER UNIV PRESS2019

Cell-autonomous inflammation of BRCA1-deficient ovarian cancers drives both tumor-intrinsic immunoreactivity and immune resistance via STING

George Coukos, Christian Iseli, Alexandre Harari, Alizée Jéléna Grimm, Julien Dorier, Mauro Delorenzi

In this study, we investigate mechanisms leading to inflammation and immunoreactivity in ovarian tumors with homologous recombination deficiency (HRD). BRCA1 loss is found to lead to transcriptional reprogramming in tumor cells and cell-intrinsic inflammat ...

2021

Integrated proteogenomic deep sequencing and analytics accurately identify non-canonical peptides in tumor immunopeptidomes

Didier Trono, George Coukos, Marion Leleu, Evaristo Jose Planet Letschert, Alexandre Harari, Delphine Yvette L Grun, Markus Müller, Florian Huber, Laia Simo Riudalbas

Efforts to precisely identify tumor human leukocyte antigen (HLA) bound peptides capable of mediating T cell-based tumor rejection still face important challenges. Recent studies suggest that non-canonical tumor-specific HLA peptides derived from annotated ...

Nature Research2020

Microfluidic Device for Droplet Pairing by Combining Droplet Railing and Floating Trap Arrays

Philippe Renaud, George Coukos, Alexandre Harari, Margaux Catherine Marie Duchamp

Droplet microfluidics are characterized by the generation and manipulation of discrete volumes of solutions, generated with the use of immiscible phases. Those droplets can then be controlled, transported, analyzed or their content modified. In this wide d ...

MDPI2021

Personalized cancer vaccine strategy elicits polyfunctional T cells and demonstrates clinical benefits in ovarian cancer

George Coukos, Alexandre Harari, Florian Huber, Christine Göpfert

T cells are important for controlling ovarian cancer (OC). We previously demonstrated that combinatorial use of a personalized whole-tumor lysate-pulsed dendritic cell vaccine (OCDC), bevacizumab (Bev), and cyclophosphamide (Cy) elicited neoantigen-specifi ...

2021

Label-free identification of activated T lymphocytes through tridimensional microsensors on chip

Carlotta Guiducci, George Coukos, Alexandre Harari, Elena Bianchi, Enrico Tenaglia, Enrica Rollo

Label-free approaches to assess cell properties ideally suit the requirements of cell-based therapeutics, since they permit to characterize cells with minimal perturbation and manipulation, at the benefit of sample recovery and re-employment for treatment. ...

Elsevier2017

Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations

Clarisse Jeanne Antonia Benoit, Philippe Renaud, George Coukos, Alexandre Harari, Thamani Dahoun, Margaux Catherine Marie Duchamp

In this study we present a novel microfluidic hydrodynamic trapping device to probe the cell–cell interaction between all cell samples of two distinct populations. We have exploited an hydrodynamic trapping method using microfluidics to immobilize a batch ...

2019

Machine learning predictions of MHC-II specificities reveal alternative binding mode of class II epitopes

Amédé Noredine Larabi, George Coukos, Florence Pojer, Alexandre Harari, Kelvin Ka Ching Lau, Julien Racle, David Gfeller, Philippe Guillaume Coune

CD4+T cells orchestrate the adaptive immune response against pathogens and cancer by recognizing epitopes presented on class II major histocompatibility complex (MHC-II) molecules. The high polymorphism of MHC-II genes represents an important hurdle toward ...

CELL PRESS2023

Tumor-specific cytolytic CD4 T cells mediate immunity against human cancer

Hatice Altug, Alexander Mathis, George Coukos, David Gfeller, Pedro Romero, Alexandre Harari, Xiaokang Li, Yen-Cheng Liu

CD4 T cells have been implicated in cancer immunity for their helper functions. Moreover, their direct cytotoxic potential has been shown in some patients with cancer. Here, by mining single-cell RNA-seq datasets, we identified CD4 T cell clusters displayi ...

2021

Biotechnologies to tackle the challenge of neoantigen identification

Philippe Renaud, George Coukos, Alexandre Harari, Margaux Catherine Marie Duchamp

Among immune correlates of clinical responses, tumor-specificneoantigens took the spotlight as relevant targets for cancerimmunotherapy. The implementation of pipelines forpersonalized cancer therapy remains challenging due to theprivacy, that is patient-s ...

2020

Unsupervised Analysis of Flow Cytometry Data in a Clinical Setting Captures Cell Diversity and Allows Population Discovery

George Coukos, Nicolas Jean Philippe Guex, Fabrizio Benedetti, Alexandre Harari

2021

Tumor-Specific Cytolytic Cd4 T Cells Mediate Protective Immunity Against Human Cancer

Hatice Altug, Alexander Mathis, George Coukos, David Gfeller, Pedro Romero, Alexandre Harari, Xiaokang Li, Yen-Cheng Liu

BMJ PUBLISHING GROUP2020