Publications by Tomas Lukes | EPFL Graph Search

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Background: Structured illumination microscopy (SIM) is a family of methods in optical fluorescence microscopy that can achieve both optical sectioning and super-resolution effects. SIM is a valuable method for high-resolution imaging of fixed cells or tis ...

Most diffraction-unlimited super-resolution imaging critically depends on the switching of fluorophores between at least two states, often induced using intense laser light and specialized buffers or UV radiation. Recently, so-called self-blinking dyes tha ...

Super-resolution structured illumination microscopy (SR-SIM) can be conducted at video-rate acquisition speeds when combined with high-speed spatial light modulators and sCMOS cameras, rendering it particularly suitable for live-cell imaging. If, however, ...

The stability of fusion was evaluated by its breakage when interocular blur differences were presented under vergence demand to healthy subjects. We presumed that these blur differences cause suppression of the more blurred image (interocular blur suppress ...

Focal adhesions are complicated assemblies of hundreds of proteins that allow cells to sense their extracellular matrix and adhere to it. Although most focal adhesion proteins have been identified, their spatial organization in living cells remains challen ...

Surface molecules, distributed in diverse patterns and clusters on cell membranes, influence vital functions of living cells. It is therefore important to understand their molecular surface organisation under different physiological and pathological condit ...

SIMToolbox is an open-source, modular set of functions for MATLAB equipped with a user-friendly graphical interface and designed for processing two-dimensional and three-dimensional data acquired by structured illumination microscopy (SIM). Both optical se ...

Sub-diffraction or super-resolution fluorescence imaging allows the visualization of the cellular morphology and interactions at the nanoscale. Statistical analysis methods such as super-resolution optical fluctuation imaging (SOFI) obtain an improved spat ...

Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenge for super-resolution microscopy. Here we address this important issue by combining photoactivated localization microscopy (PALM) with super-res ...

Super-resolution techniques expand the abilities of researchers who have the knowledge and resources to either build or purchase a system. This excludes the part of the research community without these capabilities. Here we introduce the openSIM add-on to ...

We introduce and demonstrate a new high performance image reconstruction method for super-resolution structured illumination microscopy based on maximum a posteriori probability estimation (MAP-SIM). Imaging performance is demonstrated on a variety of fluo ...

With the widespread uptake of two-dimensional (2D) and three-dimensional (3D) single-molecule localization microscopy (SMLM), a large set of different data analysis packages have been developed to generate super-resolution images. In a large community effo ...

Optical system comprising a synthetic aperture generating unit; said synthetic aperture generating unit being adapted for generating several apertures, which are correlated by interference for improving contrast and/or resolution based on the interference ...

Tomas Lukes

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Imaging tissues and cells beyond the diffraction limit with structured illumination microscopy and Bayesian image reconstruction

Self-Blinking Dyes Unlock High-Order and Multiplane Super-Resolution Optical Fluctuation Imaging

High-speed multiplane structured illumination microscopy of living cells using an image-splitting prism

Influence of Artificially Generated Interocular Blur Difference on Fusion Stability Under Vergence Stress

Combining PALM and SOFI for quantitative imaging of focal adhesions in living cells

Quantifying protein densities on cell membranes using super-resolution optical fluctuation imaging

SIMToolbox: a MATLAB toolbox for structured illumination fluorescence microscopy

Smoothness correction for better SOFI imaging

Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions

Open-source microscope add-on for structured illumination microscopy

Three-dimensional super-resolution structured illumination microscopy with maximum a posteriori probability image estimation

Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software

Superresolved synthetic aperture microscope