Kyle Michael Douglass

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Three-dimensional (3D) localization-based super-resolution microscopy (SR) requires correction of aberrations to accurately represent 3D structure. Here we show how a depth-dependent lateral shift in the apparent position of a fluorescent point source, whi ...

Biological processes are inherently multi-scale, and supramolecular complexes at the nanoscale determine changes at the cellular scale and beyond. Single-molecule localizationmicroscopy (SMLM)(1-3) techniques have been established as important tools for st ...

A Koehler integrator device (10) comprises a collimating lens (11) being arranged for collimating a light field created by an incoherent or partially coherent light source, a pair of planar first and second micro-lens arrays (12, 13) being arranged for rel ...

Super-resolution microscopies have become an established tool in biological research. However, imaging throughput remains a main bottleneck in acquiring large datasets required for quantitative biology. Here we describe multifocal flat illumination for fie ...

Chromatin is organized into topologically associating domains (TADs) enriched in distinct histone marks. In cancer, gain-of-function mutations in the gene encoding the enhancer of zeste homolog 2 protein (EZH2) lead to a genome-wide increase in histone-3 L ...

Super-resolution fluorescence microscopy improves spatial resolution, but this comes at a loss of image throughput and presents unique challenges in identifying optimal acquisition parameters. Microscope automation routines can offset these drawbacks, but ...

Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reco ...

Kyle Michael Douglass

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Correction of a Depth-Dependent Lateral Distortion in 3D Super-Resolution Imaging

Super-resolution imaging of multiple cells by optimized flat-field epi-illumination

Koehler integrator device and application thereof in a multi-focal confocal microscope

Homogeneous multifocal excitation for high-throughput super-resolution imaging

EZH2 oncogenic mutations drive epigenetic, transcriptional, and structural changes within chromatin domains

Autonomous illumination control for localization microscopy

Multicolor single-particle reconstruction of protein complexes

Multicolor Single-Particle Reconstruction of Protein Complexes

Nanoscale Spatial Organization of Chromatin in its Cellular Context, from Telomeres to Hox