Publications by Daniele Mammoli

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Para-water is an analogue of para-hydrogen, where the two proton spins are in a quantum state that is antisymmetric under permutation, also known as singlet state. The populations of the nuclear spin states in para-water are believed to have long lifetimes ...

Hyperpolarization by dissolution dynamic nuclear polarization (D-DNP) offers a way of enhancing NMR signals by up to five orders of magnitude in metabolites and other small molecules. Nevertheless, the lifetime of hyperpolarization is inexorably limited, a ...

Nuclear Magnetic Resonance (NMR) is one of the most versatile techniques since it enables the characterization of solid, liquid and gaseous systems in a plethora of in-vitro and in-vivo experiments. Despite its multidisciplinary scope, it still suffers fro ...

Transverse and longitudinal relaxation times (T11 and T1) have been widely exploited in NMR to probe the binding of ligands and putative drugs to target proteins. We have shown recently that long-lived states (LLS) can be more sensitive to ligand binding. ...

By monitoring the effect of deuterium decoupling on the decay of transverse N-15 magnetization in D-N-15 spin pairs during multiple-refocusing echo sequences, we have determined fast D-D exchange rates k(D) and compared them with fast H-H exchange rates k( ...

Ligands that have an affinity for protein targets can be screened very effectively by exploiting favorable properties of long-lived states (LLS) in NMR spectroscopy. In this work, we describe the use of LLS for competitive binding experiments to measure ac ...

In gas phase, collisions that affect the rotational angular momentum lead to the return of the magnetization to its equilibrium (relaxation) in Nuclear Magnetic Resonance (NMR). To the best of our knowledge, the longitudinal relaxation rates R-1 = 1/T-1 of ...

We show that an imbalance between the populations of singlet (S) and triplet (T) states in pairs of magnetically equivalent spins can be generated by dissolution dynamic nudear polarization: In partly deuterated ethanol ((CD3CH2OD)-C-13), this T/S imbalanc ...

Although nuclear magnetic resonance (NMR) can provide a wealth of information, it often suffers from a lack of sensitivity. Dynamic Nuclear Polarization (DNP) provides a way to increase the polarization and hence the signal intensities in NMR spectra by tr ...

The lifetimes T-LLS of long-lived states or T-LLC of long-lived coherences can be used for the accurate determination of dissociation constants of weak protein ligand complexes. The remarkable contrast between signals derived from LLS or LLC in free and bo ...

We have induced hyperpolarized long-lived states in compounds containing C-13-bearing methyl groups by dynamic nuclear polarization (DNP) at cryogenic temperatures, followed by dissolution with a warm solvent. The hyper polarized methyl long-lived states g ...

Daniele Mammoli

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Challenges in preparing, preserving and detecting para-water in bulk: overcoming proton exchange and other hurdles

Long-Lived States of Magnetically Equivalent Spins Populated by Dissolution-DNP and Revealed by Enzymatic Reactions

Long-lived nuclear spin states of magnetically equivalent protons by means of dissolution dynamic nuclear polarization

Drug Screening Boosted by Hyperpolarized Long-Lived States in NMR

Kinetic isotope effects for fast deuterium and proton exchange rates

Exploring Weak Ligand-Protein Interactions by Long-Lived NMR States: Improved Contrast in Fragment-Based Drug Screening

Collisional cross-section of water molecules in vapour studied by means of H-1 relaxation in NMR

Hyperpolarized para-Ethanol

Dynamic Nuclear Polarization and Other Magnetic Ideas at EPFL

Ligand-Protein Affinity Studies Using Long-Lived States of Fluorine-19 Nuclei

Dynamic Nuclear Polarization of Long-Lived Nuclear Spin States in Methyl Groups