Publications by Romain Guiet | EPFL Graph Search

Related publications (19)

Podosomes, But not the Maturation status, Determine the Protease-Dependent 3D Migration in human Dendritic cells

Dendritic cells (DC) are professional Antigen-Presenting Cells scattered throughout antigen-exposed tissues and draining lymph nodes, and survey the body for pathogens. Their ability to migrate through tissues, a 3D environment, is essential for an effecti ...

2018

Cellular Uptake and Intracellular Trafficking of Poly(N-(2-Hydroxypropyl) Methacrylamide)

Anders Meibom, Harm-Anton Klok, Graham Knott, Stéphane Laurent Escrig, Olivier Burri, Arne Seitz, Romain Guiet, Claudia Battistella

Cellular uptake and intracellular trafficking of polymer conjugates or polymer nanoparticles is typically monitored using fluorescence-based techniques such as confocal microscopy. While these methods have provided a wealth of insight into the internalizat ...

American Chemical Society2019

Microarrayed human bone marrow organoids for modeling blood stem cell dynamics

Matthias Lütolf, Nathalie Brandenberg, Romain Guiet, Sonja Giger, Moritz Hofer

In many leukemia patients, a poor prognosis is attributed either to the development of chemotherapy resistance by leukemic stem cells (LSCs) or to the inefficient engraftment of transplanted hematopoietic stem/progenitor cells (HSPCs) into the bone marrow ...

AIP Publishing2022

Antibody-Mediated Trapping of Helminth Larvae Requires CD11b and Fc gamma Receptor I

Nicola Harris, Romain Guiet, Arne Seitz, Beatrice Volpe, Julia Esser-von Bieren, Duncan-Bruce Sutherland, Manuel Kulagin

Infections with intestinal helminths severely impact on human and veterinary health, particularly through the damage that these large parasites inflict when migrating through host tissues. Host immunity often targets the motility of tissue-migrating helmin ...

American Association of Immunologists ; Oxford University Press2015

Learning-related congruent and incongruent changes of excitation and inhibition in distinct cortical areas

Carl Petersen, Sylvain Crochet, Romain Guiet, Georgios Foustoukos, Vahid Esmaeili, Yanqi Liu, Keita Tamura, Anastasiia Oryshchuk

Excitatory and inhibitory neurons in diverse cortical regions are likely to contribute differentially to the transformation of sensory information into goal-directed motor plans. Here, we investigate the relative changes across mouse sensorimotor cortex in ...

2022

Hookworms Evade Host Immunity by Secreting a Deoxyribonuclease to Degrade Neutrophil Extracellular Traps

Nicola Harris, Romain Guiet, Arne Seitz, Beatrice Volpe, Tiffany Babette Angélique Bouchery, Kathleen Shah, Mati André Marie Moyat, Manuel Kulagin

Hookworms cause a major neglected tropical disease, occurring after larvae penetrate the host skin. Neutrophils are phagocytes that kill large pathogens by releasing neutrophil extracellular traps (NETs), but whether they target hookworms during skin infec ...

CELL PRESS2020

An Open-Source Whole Slide Image Registration Workflow at Cellular Precision Using Fiji, QuPath and Elastix

Olivier Burri, Romain Guiet, Arne Seitz, Nicolas René Chiaruttini, Jessica Sordet

Image analysis workflows for Histology increasingly require the correlation and combination of measurements across several whole slide images. Indeed, for multiplexing, as well as multimodal imaging, it is indispensable that the same sample is imaged multi ...

FRONTIERS MEDIA SA2022

Biotin-NeutrAvidin Mediated Immobilization of Polymer Micro- and Nanoparticles on T Lymphocytes

Harm-Anton Klok, Olivier Burri, Romain Guiet, Arne Seitz, Maxime Ayer

Cells are powerful carriers that can help to improve the delivery of nanomedicines. One approach to use cells as carriers is to immobilize the nanoparticulate cargo on the cell surface. While a plethora of chemical conjugation strategies are available to b ...

2021

A Derived Positional Mapping of Inhibitory Subtypes in the Somatosensory Cortex

Henry Markram, Felix Schürmann, Daniel Keller, Olivier Burri, Romain Guiet, Julie Meystre

Obtaining a catalog of cell types is a fundamental building block for understanding the brain. The ideal classification of cell-types is based on the profile of molecules expressed by a cell, in particular, the profile of genes expressed. One strategy is, ...

2019

Injury-induced intestinal stem cell renewal requires capillary morphogenesis gene 2

Françoise Gisou van der Goot Grunberg, Nathalie Brandenberg, Olivier Burri, Romain Guiet, Julien Léonard Duc, Béatrice Kunz, Lucie Josepha Marie Bracq, Audrey Geneviève Chuat

Patients with the rare genetic disorder Hyaline Fibromatosis Syndrome (HFS) often succumb before 18 months of age due to severe diarrhea. As HFS is caused by loss-of-function mutations in the gene encoding capillary morphogenesis gene 2 (CMG2), these sympt ...

Springer Science and Business Media LLC2025

Capturing Cardiogenesis in Gastruloids

Matthias Lütolf, Romain Guiet, Nicolas Antoine Vincent Broguiere, Mehmet Ugur Girgin, Giuliana Rossi, Andrea Boni

Organoids are powerful models for studying tissue development, physiology, and disease. However, current culture systems disrupt the inductive tissue-tissue interactions needed for the complex morphogenetic processes of native organogenesis. Here, we show ...

CELL PRESS2021

Antibodies Trap Tissue Migrating Helminth Larvae and Prevent Tissue Damage by Driving IL-4Rα-Independent Alternative Differentiation of Macrophages

Nicola Harris, Alessandra Piersigilli, Romain Guiet, Arne Seitz, Beatrice Volpe, Ilaria Mosconi, Julia Esser-von Bieren, Fei Chen

Approximately one-third of the world's population suffers from chronic helminth infections with no effective vaccines currently available. Antibodies and alternatively activated macrophages (AAM) form crucial components of protective immunity against chall ...

Public Library of Science2013

Sphingolipids control dermal fibroblast heterogeneity

Gioele La Manno, Olivier Burri, Romain Guiet, Giovanni D'Angelo, Galina Glousker, Irina Khven, Sylvia Ho, Laura Capolupo, Alex Russell Lederer

Human cells produce thousands of lipids that change during cell differentiation and can vary across individual cells of the same type. However, we are only starting to characterize the function of these cell-to-cell differences in lipid composition. Here, ...

American Association for the Advancement of Science2022

T Cell-Mediated Transport of Polymer Nanoparticles across the Blood-Brain Barrier

Harm-Anton Klok, Olivier Burri, Romain Guiet, Arne Seitz, Maxime Ayer, Markus Schuster

Delivery of therapeutics to the central nervous system (CNS) is challenging due to the presence of the blood-brain barrier (BBB). Amongst various approaches that have been explored to facilitate drug delivery to the CNS, the use of cells that have the intr ...

WILEY2020

Correlative multicolor 3D SIM and STORM microscopy

Pierre Gönczy, Arne Seitz, Romain Guiet, Virginie Hamel, Paul Guichard, Mathias Fournier

Within the last decade, super-resolution methods that surpass the diffraction limit of light microscopy have provided invaluable insight into a variety of biological questions. Each of these approaches has inherent advantages and limitations, such that the ...

Optical Soc Amer2014

Stress granules quantification on fixed and live cells

Francesco Stellacci, Romain Guiet, Xufeng Xu

The custom script was written based on Fiji for measuring the number or size of stress granules inside fixed cells (using a fixed threshold) or live cells (using a local maximum). It allows the batch image data processing for the study of the effects of di ...

Zenodo2023

Structural and molecular characterization of astrocyte and vasculature connectivity in the mouse hippocampus and cortex

Henry Markram, Giovanna Ambrosini, Daniel Keller, Marwan Muhammad Ahmed Abdellah, Romain Guiet, Nicolas René Chiaruttini, Elvis Boci, Charlotte Lorin

The relation of astrocytic endfeet to the vasculature plays a key functional role in the neuro‐glia‐vasculature unit. We characterize the spatial organization of astrocytes and the structural aspects that facilitate their involvement in molecular exchanges ...

Wiley2024

Measuring reporter activity domain in EPI aggregates and Gastruloids.ijm

Matthias Lütolf, Olivier Burri, Romain Guiet, Mehmet Ugur Girgin

This imagej macro analyses the reporter intensity activity and expression domain in EPI aggregates and Gastruloids. ...

Zenodo2022

Applying Whole Organ Clearing Techniques To Spinal Cord Injury: A Survey Of Techniques

Grégoire Courtine, Olivier Burri, Quentin Barraud, Romain Guiet, Thierry Laroche, Newton Cho, Laura Battista

MARY ANN LIEBERT2018