Romain Guiet

Expertise Development of BioImage Analysis workflows and algorithms to extract quantitative data from microscopy images.BioSample Preparation : in vitro cell, organoid, time-lapse, fixation, staining strategies, actin, mitochondria, fluorescent protein expressionBioImage Acquisition : Widefield, Confocal, Whole Slide Imaging, Super-resolution (SIM, STORM, STED), FRET, FRAP, FLIMBioImage Analysis: Colocalisation analysis, Deconvolution, Time-lapse , Whole Slide ImagingTools & Software: ImageJ/Fiji, QuPath, ilastik, CellProfiler, Imaris, Arivis, Napari, Jupyter NotebooksProgramming languages: ImageJ macro language, Groovy, Java, Python, (R, Matlab) Mission Development of BioImage Analysis workflows and training to EPFL collaboratorsTeaching during courses on image formation, image acquisition and image analysis.Provide support and advice in the development of meaningful biological experiments where the output relies on image analysis.Develop tools and algorithms using open source tools and promote Open Science whenever possible.Disseminate SOPs on FAIR data practices.Provide support to the greater imaging community through activity on the forum.image.sc . Faculty Council Candidacy As an Imaging Scientist in a Core Facility, I am in contact with a broad community, which motivated me to join the Life Sciences Faculty Council. I am committed to collaborating with my colleagues within ELSA to bring forward their concerns  and I aim to contribute through fair representation, transparency, and constructive dialogue. Current work PTBIOP - BioImaging and Optics Core Facility Romain has been working at the BIOP since 2011 after a Ph.D. in Biology. He is responsible, along with Nicolas Chiaruttini and Rémy Dornier  for the advice and follow up of projects involving microscopy and quantification. His expertise with in vitro cell culture and imaging is also beneficial to help BIOP users with their staining strategies and experiment design/optimization.Background: Bioengineering, Ph.D. Immunology & Cancer (3D cell migration , organoid , cell-cytoskeleton) Education PhD | Immunology & Cancer 2007 – 2011 University Paul Sabatier-Toulouse III, Toulouse, FRANCE Directed by Advisor: PhD. I. Maridonneau-Parini IPBS-UMR5089.205 route de NarbonneToulouse, France M2R (eq. Research Master Degree) | Molecular and cellular biology 2006 – 2007 University Paul Sabatier-Toulouse III, Toulouse, FRANCE Biological Engineer (eq. Master Degree) | Protein Engineering 2003 – 2006 Polytech Clermont-Ferrand, Clermont-Ferrand, France Preparatory classes | (2-year intensive program preparing for the national competitive exam for entry to French engineering schools). Specialty: Biology 2001 – 2003 High School Louis Barthou, Pau, France Baccalauréat (eq. high school degree) | Scientific Grade 2000 – 2001 High School St John Perse, Pau, France Teaching & PhD Courses Image Processing for Life Science BIO-695 Registration details will be announced via email. It takes place yearly from Sept./October to December & intends to teach image processing with a strong emphasis of applications in life sciences. The idea is to enable the participants to solve image-processing questions via workflows independently. MOOC : Image Processing and Analysis for Life Science MOOC on coursewareBIO-659 - Advanced Microscopy for Life Science A PhD Program Course focusing on microscopy