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Andreas Stephan Lesch

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Related publications (58)

Fixation and Permeabilization Approaches for Scanning Electrochemical Microscopy of Living Cells

Hubert Girault, Horst Pick, Fernando Cortes Salazar, Andreas Stephan Lesch, Alexandra Bondarenko, Petar Stupar, Tzu-En Lin

Scanning electrochemical microscopy (SECM) has been widely used for the electrochemical imaging of dynamic topographical and metabolic changes in alive adherent mammalian cells. However, extracting intracellular information by SECM is challenging, since it ...

American Chemical Society2016

Untersuchung der Tyrosinase-Expression in nicht-metastatischen und metastatischen Melanomgeweben durch elektrochemische Rastersondenmikroskopie

Hubert Girault, Horst Pick, Fernando Cortes Salazar, Andreas Stephan Lesch, Alexandra Bondarenko, Tzu-En Lin

Trotz des enormen Fortschritts, der bei der Diagnose von Melanomen erzielt wurde, bleibt die zuverlässige Erkennung der verschiedenen Malignomstadien eine Herausforderung. Derzeitige Strategien setzen auf die optische Erfassung der Konzentration und räumli ...

Wiley-Blackwell2016

Inkjet Printing Meets Electrochemical Energy Conversion

Hubert Girault, Fernando Cortes Salazar, Véronique Amstutz, Andreas Stephan Lesch, Victor Costa Bassetto

Inkjet printing is a very powerful digital and mask-less microfabrication technique that has attracted the attention of several research groups working on electrochemical energy conversion concepts. In this short review, an overview is given about recent e ...

2015

Electrochemical push-pull probe: from scanning electrochemical microscopy (SECM) to multimodal altering of cell microenvironment

Hubert Girault, Fernando Cortes Salazar, Dmitry Momotenko, Andreas Stephan Lesch, Alexandra Bondarenko

To understand biological processes at the cellular level, a general approach is to alter the cells’ environment and to study their chemical responses. Herein, we present the implementation of an electrochemical push-pull probe, which combines a microfluidi ...

Amer Chemical Soc2015

Inkjet Printed Nanohydrogel Coated Carbon Nanotubes Electrodes For Matrix Independent Sensing

Hubert Girault, Fernando Cortes Salazar, Véronique Amstutz, Andreas Stephan Lesch

Polyacrylamide (PA) based hydrogels are used in several applications including polyacrylamide gel electrophoresis and sensing devices. Homogeneous and compact PA films can be prepared based on chemical or photopolymerization processes. However, the accurat ...

2015

Electrochemical Detection of Free Chlorine at Inkjet Printed Silver Electrodes

Hubert Girault, Hongyan Bi, Fernando Cortes Salazar, Véronique Amstutz, Andreas Stephan Lesch, Milica Jovic

A low-cost, reliable and sensitive electrochemical method for free chlorine analysis in water using inkjet printed silver electrodes is presented. Free chlorine detection was based on linear sweep voltammetry (LSV) analysis of AgCl/Ag2O films formed over a ...

Elsevier Science Sa2015

Multiple scanning electrochemical microscopy mapping of tyrosinase in micro-contact printed fruit samples on polyvinylidene fluoride membrane

Hubert Girault, Fernando Cortes Salazar, Liang Qiao, Andreas Stephan Lesch, Alexandra Bondarenko, Tzu-En Lin

Herein, we introduce three orthogonal and compatible methods for detecting tyrosinase, a key factor in fruit browning and skin disorders, with high spatial resolution by means of scanning electrochemical microscopy (SECM). All methods are performed subsequ ...

Elsevier2015

Finger Probe Array for Topography-Tolerant Scanning Electrochemical Microscopy of Extended Samples

Hubert Girault, Gunther Wittstock, Fernando Cortes Salazar, Dmitry Momotenko, Andreas Stephan Lesch

Scanning electrochemical microscopy with soft microelectrode array probes has recently been used to enable reactivity imaging of extended areas and to compensate sample corrugation perpendicular to the scanning direction. Here, the use of a new type of mic ...

Amer Chemical Soc2014

Rapid optimisation of a lactate biosensor design using soft probes scanning electrochemical microscopy

Hubert Girault, Fernando Cortes Salazar, Andreas Stephan Lesch

We report the mapping of biocatalytically active surfaces, particularly on an express search for optimal immobilization conditions of the enzyme lactate oxidase by means of scanning electrochemical microscopy (SECM). With this aim, soft stylus SECM probes ...

Elsevier2014

Electrostatic Spray Ionization Mass Spectrometry Imaging

Hubert Girault, Horst Vogel, Horst Pick, Liang Qiao, Elena Tobolkina, Andreas Stephan Lesch, Alexandra Bondarenko, Xiaoqin Zhong

Imaging samples on a surface by mass spectrometry (MS) requires the combination of MS detection with a scanning mode that enables localized desorption and ionization and/or detection of sample analytes with good spatial resolution. We have developed a new ...

Amer Chemical Soc2014

Large scale inkjet-printing of carbon nanotubes electrodes for antioxidant assays in blood bags

Hubert Girault, Michel Prudent, Niels Erik Lion, Fernando Cortes Salazar, Andreas Stephan Lesch

Herein, we present the large scale fabrication of carbon nanotubes (CNT) electrodes supported on flexible polymeric sheets by subsequent multilayer inkjet printing of a silver layer for electrical connection, CNT layers as active electrode material and an ...

Elsevier2014

Electrochemical Pseudo-Titration of Water-Soluble Antioxidants

Hubert Girault, Alice Adei Neequaye, Fernando Cortes Salazar, Andreas Stephan Lesch

An amperometric test for the antioxidant power (AOP) of biological and food samples is presented. The gist of the method is to measure by linear sweep voltammetry the anodic current produced during the oxidation of the labile species present in the sample ...

Wiley-Blackwell2013

High-throughput scanning electrochemical microscopy brushing of strongly tilted and curved surfaces

Hubert Girault, Gunther Wittstock, Fernando Cortes Salazar, Dmitry Momotenko, Andreas Stephan Lesch

The feasibility of high-throughput scanning electrochemical microscopy (SECM) of strongly tilted (tilt angles ≤4°) and curved substrates (diameter of curvature ≥9 cm) is demonstrated by brushing them with a soft linear array of carbon microelectrodes. This ...

Elsevier2013

Electrochemical Push–Pull Scanner with Mass Spectrometry Detection

Hubert Girault, Gunther Wittstock, Fernando Cortes Salazar, Liang Qiao, Dmitry Momotenko, Andreas Stephan Lesch

This manuscript presents a push-pull electrochem. scanner able to image reactivity of initially dry surfaces by scanning electrochem. microscopy (SECM) and to probe mols. present or generated at the surface by mass spectrometry (MS). The proof-of-concept i ...

Amer Chemical Soc2012

Microfluidic Push-Pull Probe for Scanning Electrochemical Microscopy

Hubert Girault, Gunther Wittstock, Fernando Cortes Salazar, Dmitry Momotenko, Andreas Stephan Lesch

This paper presents a microfluidic push-pull probe for scanning electrochemical microscopy (SECM) consisting of a working microelectrode, an integrated counter/reference electrode and two microchannels for pushing and pulling an electrolyte solution to and ...

2011

Seeing Big with Scanning Electrochemical Microscopy

Hubert Girault, Gunther Wittstock, Fernando Cortes Salazar, Dmitry Momotenko, Andreas Stephan Lesch

Specialized microelectrode probes fabricated in a soft polymer film now make it possible to use scanning electrochemical microscopy to image the reactivity of large, corrugated, tilted, and dry surfaces. ...

American Chemical Society2011

Soft Microelectrode Linear Array for Scanning Electrochemical Microscopy

Hubert Girault, Gunther Wittstock, Fernando Cortes Salazar, Dmitry Momotenko, Andreas Stephan Lesch

A linear array of eight individual addressable microelectrodes has been developed in order to perform highthroughput scanning electrochemical microscopy (SECM) imaging of large sample areas in contact regime. Similar to previous reports, the soft microelec ...

American Chemical Society2010

Fountain pen for scanning electrochemical microscopy

Hubert Girault, Gunther Wittstock, Fernando Cortes Salazar, Dmitry Momotenko, Andreas Stephan Lesch

A fountain pen probe has been developed to extend the scope of scanning electrochemical microscopy (SECM) experiments to dry surfaces. The fountain pen is fabricated by UV-photoablation of a polyethylene terephthalate (PET) film and consists on one side of ...

Royal Society of Chemistry2010

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