Publications by Benoît Vianay

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The mechanical interaction between adherent cells and their substrate relies on the formation of adhesion sites and on the stabilization of contractile acto-myosin bundles, or stress fibers. The shape of the cell and the orientation of these fibers can be ...

We present a novel approach to modeling cell spreading, and use it to reveal a potentially central mechanism regulating focal adhesion maturation in various cell phenotypes. Actin bundles that span neighboring focal complexes at the lamellipodium-lamellum ...

We propose a new technique to measure the volume of adherent migrating cells. The method is based on a negative staining where a fluorescent, non-cell-permeant dye is added to the extracellular medium. The specimen is observed with a conventional fluoresce ...

Actin-myosin filament bundles (stress fibers) are critical for tension generation and cell shape, but their mechanical properties are difficult to access. Here we propose a novel approach to probe individual peripheral stress fibers in living cells through ...

Actin-myosin microfilament bundles or stress-fibers are the principal tension-generating structures in the cell. Their mechanical properties are critical for cell shape, motion, and interaction with other cells and extracellular matrix, but were so far dif ...

The mechanical coupling between adherent cells and their substrates is a major driver of downstream behavior. This coupling relies on the formation of adhesion sites and actin bundles. How cells generate these elements remains only partly understood. A pot ...

Communication between vascular smooth muscle cells (SMCs) allows control of their contraction and so regulation of blood flow. The contractile state of SMCs is regulated by cytosolic Ca2+ concentration (Ca2+) which propagates as Ca2+ waves over a sign ...

Benoît Vianay

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Cell Shape Dynamics Reveal Balance of Elasticity and Contractility in Peripheral Arcs

Numerically bridging lamellipodial and filopodial activity during cell spreading reveals a potentially novel trigger of focal adhesion maturation

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Microsurgery-aided in-situ force probing reveals extensibility and viscoelastic properties of individual stress fibers

Ultra-soft cantilevers and 3-D micro-patterned substrates for contractile bundle tension measurement in living cells

An actin length threshold regulates adhesion maturation at the lamellipodium/lamellum interface

Ultrafast Ca2+ wave in cultured vascular smooth muscle cells aligned on a micropatterned surface

Evaluating Tension in Actomyosin Bundles at the Cell Periphery

Actin Bundle Stabilization During Cell Spreading on Micropatterned Substrates