Publications by Suliana Manley

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Single-molecule localization microscopy (SMLM) is a powerful tool that is routinely used for nanoscale optical imaging of biological samples. Recently, this approach has been applied to study optically active defects in two-dimensional (2D) materials. Such ...

Transcription factors (TFs) regulate gene expression in both prokaryotes and eukaryotes by recognizing and binding to specific DNA promoter sequences. In higher eukaryotes, it remains unclear how the duration of TF binding to DNA relates to downstream tran ...

The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. The prevailing challenge in live-cell fluorescence microscopy is capturing intra-cellular dynamics while preservi ...

Mitochondrial fission is a highly regulated process that, when disrupted, can alter metabolism, proliferation and apoptosis(1-3). Dysregulation has been linked to neurodegeneration(3,4), cardiovascular disease(3) and cancer(5). Key components of the fissio ...

Fluorescent D-amino acids (FDAAs) have previ-ously been developed to enable in situ highlighting of locations of bacterial cell wall growth. Most bacterial cells lie at the edge of the diffraction limit of visible light; thus, resolving the precise details ...

Localization microscopy achieves nanoscale spatial resolution by iterative localization of sparsely activated molecules, which generally leads to a long acquisition time. By implementing advanced algorithms to treat overlapping point spread functions (PSFs ...

The NLRP3 inflammasome is a central component of the innate immune system. Its activation leads to for-mation of the ASC speck, a supramolecular assembly of the inflammasome adaptor protein ASC. Different models, based on ASC overexpression, have been prop ...

Three-dimensional (3D) localization-based super-resolution microscopy (SR) requires correction of aberrations to accurately represent 3D structure. Here we show how a depth-dependent lateral shift in the apparent position of a fluorescent point source, whi ...

Interferometric scattering (iSCAT) microscopy enables the label-free observation of biomolecules. Consequently, single-particle imaging and tracking with the iSCAT-based method known as mass photometry (MP) is a growing area of study. However, establishing ...

Optical flat-fielding systems, such as field-mapping or integration-based beam shapers, are used to transform nonuniform illumination into uniform illumination. Thus, flat-fielding paves the way for imaging that is independent of position within a field of ...

Protein concentration gradients pattern developing organisms and single cells. In Schizosaccharomyces pombe rod-shaped cells, Pom1 kinase forms gradients with maxima at cell poles. Pom1 controls the timing of mitotic entry by inhibiting Cdr2, which forms s ...

Biological processes are inherently multi-scale, and supramolecular complexes at the nanoscale determine changes at the cellular scale and beyond. Single-molecule localizationmicroscopy (SMLM)(1-3) techniques have been established as important tools for st ...

Fluorescence microscopy methods have been developed to circumvent the diffraction limit by exploiting nonlinearities in the interactions between light and fluorophores. Initially, these methods were up to orders of magnitude slower than standard microscopi ...

The HIV structural protein Gag assembles to form spherical particles of radius ∼70 nm. During the assembly process, the number of Gag proteins increases over several orders of magnitude from a few at nucleation to thousands at completion. The challenge in ...

DNA replication in bacteria takes place on highly compacted chromosomes, where segregation, transcription, and repair must occur simultaneously. Within this dynamic environment, colocalization of sister replisomes has been observed in many bacterial specie ...

Super-resolution localization microscopy methods such as PALM and STORM have been shown to provide imaging with resolutions up to a few tens of nanometers while using relatively simple setups. Biplane PALM has extended the PALM technique to three-dimension ...

Super-resolution imaging methods have revolutionized fluorescence microscopy by revealing the nanoscale organization of labeled proteins. In particular, single-molecule methods such as Stochastic Optical Reconstruction Microscopy (STORM) provide resolution ...

Localization microscopy such as STORM/PALM can achieve a nanometer scale spatial resolution by iteratively localizing fluorescence molecules. It was shown that imaging of densely activated molecules can accelerate temporal resolution which was considered a ...

Event-driven acquisition uses neural-network-based recognition of specific biological events to trigger switching between slow and fast super-resolution imaging, enriching the capture of interesting events with high spatiotemporal resolution. A common goal ...

Mitotic bookmarking transcription factors remain bound to chromosomes during mitosis and were proposed to regulate phenotypic maintenance of stem and progenitor cells at the mitosis-to-G1 (M-G1) transition. However, mitotic bookmarking remains largely unex ...

Suliana Manley

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Waveguide-Based Platform for Large-FOV Imaging of Optically Active Defects in 2D Materials

Single-molecule dynamics and genome-wide transcriptomics reveal that NF-kB (p65)-DNA binding times can be decoupled from transcriptional activation

Live-cell imaging powered by computation

Distinct fission signatures predict mitochondrial degradation or biogenesis

Fluorescent D-Amino Acids for Super-resolution Microscopy of the Bacterial Cell Wall

3D high-density localization microscopy using hybrid astigmatic/biplane imaging and sparse image reconstruction

Nanoscale organization of the endogenous ASC speck

Correction of a Depth-Dependent Lateral Distortion in 3D Super-Resolution Imaging

Surface passivation and functionalisation for mass photometry

Characterization of flat-fielding systems for quantitative microscopy

Multi-phosphorylation reaction and clustering tune Pom1 gradient mid-cell levels according to cell size

Super-resolution imaging of multiple cells by optimized flat-field epi-illumination

Strategies for increasing the throughput of super-resolution microscopies

Quantitative super-resolution imaging reveals protein stoichiometry and nanoscale morphology of assembling HIV-Gag virions

Chromosome organization shapes replisome dynamics in Caulobacter crescentus

A Psf-Based Approach To Biplane Calibration In 3D Super-Resolution Microscopy

Resolution Doubling in 3D-STORM Imaging through Improved Buffers

Fast live cell imaging at nanometer scale using annihilating filter based low rank Hankel matrix approach

Event-driven acquisition for content-enriched microscopy

A role for mitotic bookmarking of SOX2 in pluripotency and differentiation