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Stewart Cole

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Related publications (266)

Streptomycin-Starved Mycobacterium tuberculosis 18b, a Drug Discovery Tool for Latent Tuberculosis

Stewart Cole, Neeraj Dhar, Claudia Sala, Ruben Hartkoorn, Ming Zhang

Mycobacterium tuberculosis 18b, a streptomycin (STR)-dependent mutant that enters a viable but nonreplicating state in the absence of STR, has been developed as a simple model for drug testing against dormant bacilli. Here, we further evaluated the STR-sta ...

Amer Soc Microbiology2012

Towards a new tuberculosis drug: pyridomycin - nature's isoniazid

Stewart Cole, Claudia Sala, Florence Pojer, Sophie Magnet, Swapna Uplekar, Ruben Hartkoorn, Joao Pedro De Almeida Neres, Raju Mukherjee

Tuberculosis, a global threat to public health, is becoming untreatable due to widespread drug resistance to frontline drugs such as the InhA-inhibitor isoniazid. Historically, by inhibiting highly vulnerable targets, natural products have been an importan ...

Wiley Open Access2012

In Vitro Activities of the New Antitubercular Agents PA-824 and BTZ043 against Nocardia brasiliensis

Stewart Cole

The in vitro activity of PA-824 and BTZ043 against 30 Nocardia brasiliensis isolates was tested. The MIC50 and MIC90 values for PA-824 were both > 64 mu g/ml. The same values for BTZ043 were 0.125 and 0.250 mu g/ml. Given the MIC values for benzothiazinone ...

2012

Molecular drug susceptibility testing and genotyping of Mycobacterium leprae strains from South America

Stewart Cole, Philippe Busso, Pushpendra Singh

Possible drug resistance in Mycobacterium leprae strains from Venezuela and three other South American countries was surveyed by molecular methods. None of the 230 strains from new leprosy cases exhibited drug resistance-associated mutations. However, two ...

2011

Probable zoonotic leprosy in the southern United States

Stewart Cole, Adamantia Kapopoulou, Jacques Rougemont, Philippe Busso, Pushpendra Singh, Rahul Sharma

In the southern region of the United States, such as in Louisiana and Texas, there are autochthonous cases of leprosy among native-born Americans with no history of foreign exposure. In the same region, as well as in Mexico, wild armadillos are infected wi ...

2011

Microbiology. Pyrazinamide--old TB drug finds new target

Stewart Cole

American Association for the Advancement of Science2011

Antimicrobial drug discovery

Stewart Cole

2011

A Case of Diffuse Lepromatous Leprosy associated with Mycobacterium lepromatosis

Stewart Cole, Philippe Busso, Pushpendra Singh

An 86-year old female patient from northeast Mexico presented with diffuse lepromatous leprosy (DLL). Sequence analysis of four genes (rrs, rpoB, sigA and hsp65) from the skin biopsy identified Mycobacterium lepromatosis. This is the first independent conf ...

2011

Comparative genomics of Esx genes from clinical isolates of Mycobacterium tuberculosis provides evidence for gene conversion and epitope variation

Stewart Cole, Swapna Uplekar, Jacques Rougemont

The 23-membered Esx protein family is involved in the host-pathogen interactions of Mycobacterium tuberculosis. These secreted proteins are among the most immunodominant antigens recognized by the human immune system and have thus been used to develop vacc ...

2011

EspD Is Critical for the Virulence-Mediating ESX-1 Secretion System in Mycobacterium tuberculosis

Stewart Cole, Neeraj Dhar, Florence Pojer, Jeffrey Chen, Stefanie Boy-Röttger

ESAT-6 system 1 (ESX-1)-mediated secretion in Mycobacterium tuberculosis is dependent on proteins encoded by the cotranscribed espA-espC-espD gene cluster. While the roles of EspA and EspC with respect to the ESX-1 secretion system have been actively inves ...

American Society for Microbiology2011

New tuberculosis drugs on the horizon

Stewart Cole

Tuberculosis (TB) remains a major global health concern whose control has been exacerbated by HIV and the emergence of multidrug-resistant (MDR-TB) and extensively drug-resistant (XDR-TB) strains of Mycobacterium tuberculosis. The demand for new and faster ...

2011

ESAT-6 secretion-independent impact of ESX-1 genes espF and espG1 on virulence of Mycobacterium tuberculosis

Stewart Cole, Jeffrey Chen

The pathogenesis of Mycobacterium tuberculosis largely depends on the secretion of the 6-kD early secreted antigenic target ESAT-6 (EsxA) and the 10-kD culture filtrate protein CFP-10 (EsxB) via the ESX-1/typeVII secretion system. Although gene products fr ...

Oxford University Press2011

Towards anti-virulence drugs targeting ESX-1 mediated pathogenesis of Mycobacterium tuberculosis

Benjamin Blasco, Stewart Cole, Florence Pojer, Jeffrey Chen

Mycobacterium tuberculosis (M. tb) the causative agent of tuberculosis (TB) causes 1.7 million deaths annually. According to the World Health Organization, in some areas of the world, 25% of TB cases are due to multi- or extensively drug resistant M. tb st ...

Elsevier2010

Clinical isolates of Mycobacterium tuberculosis in four European hospitals are uniformly susceptible to benzothiazinones

Stewart Cole

The new antitubercular drug candidate 2-[2-S-methyl-1,4-dioxa-8-azaspiro[4.5]dec-8-yl]-8-nitro-6-(trifluoromethyl)-4H-1,3-benzothiazin-4-one (BTZ043) targets the DprE1 (Rv3790) subunit of the enzyme decaprenylphosphoryl-beta-d-ribose 2'-epimerase. To monit ...

2010

A Simple Model for Testing Drugs against Non-replicating Mycobacterium tuberculosis

Stewart Cole, Neeraj Dhar, Claudia Sala, Ruben Hartkoorn, Ming Zhang

Non-replicating or dormant cells of Mycobacterium tuberculosis constitute a challenge to tuberculosis (TB) therapy because of their tolerance or phenotypic resistance to most drugs. Here, we propose a simple model for testing drugs against non-growing cell ...

2010

Effect of serial subculturing on the genetic composition and cytotoxic activity of Mycobacterium tuberculosis

Stewart Cole

Continuous subculture has been observed to produce changes in the virulence of micro-organisms, e.g. rabies virus, poliovirus and Mycobacterium bovis BCG. The latter has been used as a vaccine for tuberculosis for the last 100 years; however, in some insta ...

2010

Application of broad-spectrum resequencing microarray for genotyping rhabdoviruses

Stewart Cole, Iain Old

The rapid and accurate identification of pathogens is critical in the control of infectious disease. To this end, we analyzed the capacity for viral detection and identification of a newly described high-density resequencing microarray (RMA), termed Pathog ...

American Society for Microbiology2010

Leads for antitubercular compounds from kinase inhibitor library screens

Gerardo Turcatti, Marc Chambon, Damiano Banfi, Stewart Cole, Sophie Magnet, Ruben Hartkoorn, Edina Rita Székely

Discovering new drugs to treat tuberculosis more efficiently and to overcome multidrug resistance is a world health priority. To find antimycobacterial scaffolds, we screened a kinase inhibitor library of more than 12,000 compounds using an integrated stra ...

2010

Reconstructed ancestral sequences improve pathogen identification using resequencing DNA microarrays

Stewart Cole

We describe the benefit of using reconstructed ancestral sequences (RAS) on resequencing microarrays for rapid pathogen identification, with Enterobacteriaceae rpoB sequences as a model. Our results demonstrate a sharp improvement of call rate and accuracy ...

Public Library of Science2010

Development of a repressible mycobacterial promoter system based on two transcriptional repressors

Stewart Cole, Neeraj Dhar, Claudia Sala

Tightly regulated gene expression systems represent invaluable tools for studying gene function and for the validation of drug targets in bacteria. While several regulated bacterial promoters have been characterized, few of them have been successfully used ...

Oxford University Press2010

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