Thomas Michael Braschler

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Adult autologous human epidermal stem cells can be extensively expanded ex vivo for cell and gene therapy. Identifying the mechanisms involved in stem cell maintenance and defining culture conditions to maintain stemness is critical, because an inadequate ...

We provide a common theoretical framework reuniting specific models for the Ca2+-alginate system and general reaction diffusion theory along with experimental validation on a microfluidic chip. As a starting point, we use a set of nonlinear, partial differ ...

Microfluidic devices are increasingly used to perform biological experiments on a single-cell basis. However, long-term stability of cell positions is still an issue. A novel biocompatible method for cell entrapment and release on a microchip is presented. ...

Millimeter to centimeter-sized injectable neural scaffolds based on macroporous cryogels are presented. The polymer-scaffolds are made from alginate and carboxymethyl-cellulose by a novel simple one-pot cryosynthesis. They allow surgical sterility by means ...

There is great interest in highly sensitive separation methods capable of quickly isolating a particular cell type within a single manipulation step prior to their analysis. We present a cell sorting device based on the opposition of dielectrophoretic forc ...

We present a device capable of electrical cell lysis and evaluation of lysis efficiency in continuous flow using dielectrophoretic cell sorting. We use a combination of AC electrical fields and so-called liquid electrodes to avoid bubble creation at the el ...

Biochemical and biophysical properties instruct cardiac tissue morphogenesis. Here, we are reporting on a blend of cardiac decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen that is suitable for investigations employi ...

We present a miniaturized impedance imaging system, developed for 2D imaging of cell and tissue culture. The system is based on 16 microelectrodes (5 µm × 4 mm). An equivalent circuit for four-point (tetrapolar) impedance spectra was developed and validate ...

NOVELTY - The device has a carrier (1) which is provided with micro-cavity (2) for containing medium in direct contact with the object on which the medium is to be applied. Other carrier is provided with ring to protect the medium from environment when the ...

Alginate gel formation on-chip is presented in the first part of this thesis. The technique allows immobilization, and release, of biological cells on-chip. Furthermore, via layer by layer deposition, a locally heterogeneous environment can be generated at ...

Impedance spectroscopy is a powerful tool for label-free analysis and characterisation of living cells. In this work, we achieved the detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer. The ...

We have fabricated a microfluidic device based on a novel geometrical arrangement of lateral metal electrodes and a patterned insulator. Our device combines the concept of insulator-based “electrodeless” dielectrophoresis with multiple frequencies to achie ...

Controlling alginate gel formation by diffusion of Ca2+ ions through a filter barrier, a layer-by-layer deposition technique with resolution on the size scale of a single cell is presented. It offers the possibility of exposing cells under biocompatible co ...

Cell cycle synchronization is an important tool for the study of the cell division stages and signalling. It provides homogeneous cell cultures that are of importance to develop and improve processes such as protein synthesis and drug screening. The main a ...

A method of producing a cryogel-based multicompartment 3D scaffold is herein disclosed. The method comprises the steps of: a) providing a first frozen polymeric layer on a refrigerated support kept at subzero temperature; b) providing subsequent polymeric ...

Although hematopoietic stem cell (HSC) transplantation can restore functional hematopoiesis upon immune or chemotherapy-induced bone marrow failure, complications often arise during recovery, leading to up to 25% transplant-related mortality in treated pat ...

The invention describes a method for producing hybrid microelectronic/microfluidic sensors at industrial scale. The method is characterized in that it comprises the following steps for obtaining said microfluidic channel: a) a first lamination step of a dr ...

Dielectrophoresis is a method that has demonstrated great potential in cell discrimination and isolation. In this study, the dielectrophoretic sorting of normal and Babesia bovis infected erythrocytes was performed using a microfabricated flow cytometer. S ...

Using the concept of insulator-based "electrodeless" dielectrophoresis, we present a novel geometry for shaping electric fields to achieve lateral deviation of particles in liquid flows. The field is generated by lateral planar metal electrodes and is guid ...

Controlling alginate gel formation by diffusion of Ca ions through a filter barrier, a layer-by-layer deposition technique with resolution on the size scale of a single cell is presented. It gives the possibility of exposing cells under biocompatible condi ...

Thomas Michael Braschler

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Low temperature and mTOR inhibition favor stem cell maintenance in human keratinocyte cultures

Link between Alginate Reaction Front Propagation and General Reaction Diffusion Theory

Gentle cell trapping and release on a microfluidic chip by in situ alginate hydrogel formation

A Compressible Scaffold for Minimally Invasive Delivery of Large Intact Neuronal Networks

A miniaturized continuous dielectrophoretic cell sorter and its applications

Continuous-flow electrical lysis device with integrated control by dielectrophoretic cell sorting

Highly Efficient Cardiac Differentiation and Maintenance by Thrombin-Coagulated Fibrin Hydrogels Enriched with Decellularized Porcine Heart Extracellular Matrix

Two-dimensional impedance imaging of cell migration and epithelial stratification

Device for application of medium to human tissue or animal body such as eye, has carrier which is provided with micro-cavity for containing medium in direct contact with object on which medium is to be applied

Controlled entrapment of cells in hydrogels on chip and cell sorting by dielectrophoresis using liquid electrodes

Label-free detection of Babesia bovis infected red blood cells using impedance spectroscopy on microfabricated flow cytometer

Focusing and continuous separation of cells in a microfluidic device using lateral dielectrophoresis

Fluidic microstructuring of alginate hydrogels for the single cell niche

Tracking and synchronization of the yeast cell cycle using dielectrophoretic opacity

Cryogel 3d scaffolds and methods for producing thereof

Cryogel-based Injectable 3D Microcarrier Co-culture for Support of Hematopoietic Progenitor Niches

Microfluidic sensor and method for obtaining such a sensor

Dielectrophoretic sorting on a microfabricated flow cytometer: Label free separation of Babesia bovis infected erythrocytes

Characterization and optimization of liquid electrodes for lateral dielectrophoresis

Sequential build-up of hydrogel environments around single cells