Publications by Rudolf Rigler | EPFL Graph Search

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The variability in transcription factor concentration among cells is an important developmental determinant, yet how variability is controlled remains poorly understood. Studies of variability have focused predominantly on monitoring mRNA production noise. ...

We designed a fluorescence correlation spectroscopy (FCS) system for measurements on surfaces. The system consists of an objective-type total internal reflection fluorescence (TIRF) microscopy setup, adapted to measure FCS. Here, the fluorescence exciting ...

A two-dimensional (2-D) array (4 by 8) of single-photon avalanche diodes integrated in an industrial complementary metal-oxide-semiconductor (CMOS) process is presented. Each pixel combines a photodiode biased above its breakdown voltage in the so-called Geiger mode, a quenching resistor, and a simple comparator. The pitch between the pixels is 75 mum and the diameter of each pixel is 6.4 mum. The full integration allows reducing the number of charge carriers in a Geiger pulse. The electroluminescence respo

We demonstrate the use of laser-induced. fluorescence confocal spectroscopy to measure analyte-stimulated enhanced green fluorescent protein (egfp) synthesis by genetically modified Escherichia coli bioreporter cells. Induction is measured in cell lysates ...

The invention relates to a method and device for identifying luminescent molecules according to the fluorescence correlation spectroscopy method. The inventive method comprising the following steps: a) preparing a sample (12) containing luminescent molecul ...

Dual-color cross-correlation spectroscopy allows the detection and quantification of labeled biomolecules at ultra-low concentrations, whereby the sensitivity of the assay correlates with the measurement time. We now describe a parallel multifocal dual-col ...

A new approach to quantitative single-molecule imaging by confocal laser scanning microscopy (CLSM) is presented. it relies on fluorescence intensity distribution to analyze the molecular occurrence statistics captured by digital imaging and enables direct ...

We present parallel single molecule detection (SMD) and fluorescence correlation spectroscopy (FCS) experiments with a fully integrated complementary metal oxide semiconductor (CMOS) single-photon 2 X 2 detector array. Multifocal excitation is achieved with a diffractive optical element (DOE). Special emphasis is placed on parallelization of the total system. The performance of the novel single-photon CMOS detector is investigated and compared to a state-of-the-art single-photon detecting module [having an

The invention relates to a solid immersion lens (SIL) for a microscope, comprising a lens system with a predetermined numerical aperture. The refractive index of the material of the solid immersion lens is selected in such a way that the numerical aperture ...

We achieved photon count rates per molecule as high as with commonly used confocal fluorescence correlation spectroscopy instruments using a new total internal reflection fluorescence correlation spectroscopy system based on an epi-illumination configurati ...

This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. Objective-type TIR-FCS combines ...

Rudolf Rigler

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Control of Hox transcription factor concentration and cell-to-cell variability by an auto-regulatory switch

Total internal reflection fluorescence correlation spectroscopy (TIR-FCS) with low background and high count-rate per molecule

First fully integrated 2-D array of single-photon detectors in standard CMOS technology

Ultrasensitive reporter protein detection in genetically engineered bacteria

Identification of luminescent molecules comprises fluorescence correlation spectroscopy

Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements

Quantitative single-molecule imaging by confocal laser scanning microscopy

Parallel single molecule detection with a fully integrated single-photon 2X2 CMOS detector array

Solid immersion lens used in a microscope with a lens system is made of a material having a refractive index that is selected so that the numerical aperture of the lens system

High count rates with total internal reflection fluorescence correlation spectroscopy

Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy

Non equilibrium catalysis of single enzyme molecules

Single molecule detection at surfaces