Publication

Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy

Theo Lasser, Rudolf Rigler, Kai Hassler
2007
Journal Articles

Abstract

This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. Objective-type TIR-FCS combines the advantages of FCS with TIRF microscopy in a way that allows for simultaneous ultra-sensitive spectroscopic measurements using a single-point detector and convenient localization of single molecules on a surface by means of parallel imaging.

Official source

https://infoscience.epfl.ch/entities/publication/25d2d7df-67d9-4ad5-a6f0-9f522a5b6690

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Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy

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