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Matthias Lütolf

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Related publications (176)

Page 5 of 9

Engineering 3D cell instructive microenvironments by rational assembly of artificial extracellular matrices and cell patterning

Matthias Lütolf, Adrian Ranga

Engineered artificial microenvironments hold enormous potential as models to study developmental, physiological, pathological, and regenerative processes under highly defined conditions. Such platforms aim at bridging the gap between traditional in vitro 2 ...

2011

Substrate Elasticity Regulates Skeletal Muscle Stem Cell Self-Renewal in Culture

Matthias Lütolf, Nora Leonardi

Freshly isolated muscle stem cells (MuSCs) exhibit robust regenerative capacity in vivo that is rapidly lost in culture. Using a bioengineered substrate to recapitulate key biophysical and biochemical niche features in conjunction with a novel highly autom ...

American Association for the Advancement of Science2010

3D Inkjet Printing of Complex, Cell-Laden Hydrogel Structures

Thibaud Cherbuin, Matthias Lütolf, Andrea Negro

Inkjet printing is widely considered a promising strategy to pattern hydrogels and living cells into three-dimensional (3D) constructs that structurally resemble tissues in our body. However, this approach is currently constrained by the limited control ov ...

Nature Research2018

Multi-axial self-organization properties of mouse embryonic stem cells into gastruloids

Matthias Lütolf, Denis Duboule, Mehmet Ugur Girgin, Anne-Catherine Cossy

The emergence of multiple axes is an essential element in the establishment of the mammalian body plan. This process takes place shortly after implantation of the embryo within the uterus and relies on the activity of gene regulatory networks that coordina ...

NATURE PUBLISHING GROUP2018

Elucidating the Role of Matrix Stiffness in 3D Cell Migration and Remodeling

Matthias Lütolf, Adrian Ranga, Philipp Sebastian Lienemann, Katarzyna Mosiewicz

Reductionist in vitro model systems which mimic specific extracellular matrix functions in a highly controlled manner, termed artificial extracellular matrices (aECM), have increasingly been used to elucidate the role of cell-ECM interactions in regulating ...

Elsevier2011

The effect of matrix characteristics on fibroblast proliferation in 3D gels

Jeffrey Alan Hubbell, Matthias Lütolf

Engineering synthetic hydrogels on a molecular basis to introduce natural features that are important in instructing cell behavior is becoming increasingly crucial in biomaterial-based approaches for regenerative medicine and in cell biology to study cell– ...

Elsevier2010

Machine Learning of Hematopoietic Stem Cell Divisions from Paired Daughter Cell Expression Profiles Reveals Effects of Aging on Self-Renewal

Matthias Lütolf, Aline Roch

Changes in stem cell activity may underpin aging. However, these changes are not completely understood. Here, we combined single-cell profiling with machine learning and in vivo functional studies to explore how hematopoietic stem cell (HSC) divisions patt ...

CELL PRESS2020

Bioinspired Hydrogels for 3D Organoid Culture

Matthias Lütolf, Delphine Lorraine Perle Blondel

Organoids have stepped into the limelight as unique in vitro systems for modeling organ development, function and disease. This review provides a perspective on how chemically defined, bio-inspired hydrogels could be used for replacing ill-defined matrices ...

SWISS CHEMICAL SOC2019

Efficient high-thoughput microfluidic trapping of single cells

Philippe Renaud, Matthias Lütolf, Ana Valero

2009

Low-Defect Thiol-Michael Addition Hydrogels as Matrigel Substitutes for Epithelial Organoid Derivation

Matthias Lütolf, Saba Rezakhani, Nikolche Gjorevski

Due to their selective crosslinking chemistry and well-defined mechano-biochemical characteristics, hydrogels crosslinked by thiol-Michael addition reactions have garnered immense interest for 3D cell culture applications. These hydrogels are typically for ...

2020

A functional genetic toolbox for human tissue-derived organoids

Matthias Lütolf, Saba Rezakhani

Human organoid systems recapitulate key features of organs offering platforms for modelling developmental biology and disease. Tissue-derived organoids have been widely used to study the impact of extrinsic niche factors on stem cells. However, they are ra ...

eLIFE SCIENCES PUBL LTD2021

Devices for high-throughput aggregation and manipulation of mammalian cells

Matthias Lütolf, Nathalie Brandenberg, Sylke Höhnel

The invention concerns a device (1) for aggregating cells comprising at least one cavity (2), wherein said cavity comprises a plurality of microwells (3) for receiving at least one cell, wherein each said well comprises a vertical sidewall and a curved bot ...

2016

Integration column: Artificial ECM: expanding the cell biology toolbox in 3D

Matthias Lütolf

Many crucial cellular processes in our tissues are governed by complex, spatio-temporally regulated interactions between cells and their extracellular matrix (ECM). These interactions can be studied using well-established 3D in vitro model systems such as ...

2009

Towards high throughput drug screening for human retina organoids

Matthias Lütolf, Roland Moser, Nathalie Brandenberg

MARY ANN LIEBERT, INC2018

Organoids as tools for fundamental discovery and translation-A Keystone Symposia report

Matthias Lütolf

Complex three-dimensional in vitro organ-like models, or organoids, offer a unique biological tool with distinct advantages over two-dimensional cell culture systems, which can be too simplistic, and animal models, which can be too complex and may fail to ...

WILEY2022

Micropatterning of Hydrogels by Soft Embossing

Matthias Lütolf, Thierry Laroche, Stefan Kobel, Samy Gobaa

Conventional in situ hydrogel micropatterning techniques work successfully for relatively stiff hydrogels, but they often result in locally damaged surfaces upon demolding in the case of soft and fragile polymer networks formed at low precursor concentrati ...

American Chemical Society2009

Hydrogel-based milliwell arrays for standardized and scalable retinal organoid cultures

Matthias Lütolf, Nathalie Brandenberg

The development of improved methods to culture retinal organoids is relevant for the investigation of mechanisms of retinal development under pathophysiological conditions, for screening of neuroprotective compounds, and for providing a cellular source for ...

Nature Research2020

Synthetic fibrin-like matrix for bone tissue engineering

Jeffrey Alan Hubbell, Matthias Lütolf

2008

Probing the killing potency of tumor-infiltrating lymphocytes on microarrayed colorectal cancer tumoroids

Damien Saugy, Matthias Lütolf, Nathalie Brandenberg, Nicolas Antoine Vincent Broguiere, Krisztian Homicsko, Sylke Höhnel, François Réal Rivest, Lucie Carrié Tillard, Devanjali Dutta

Immunotherapy has emerged as a new standard of care for certain cancer patients with specific cellular and molecular makeups. However, there is still an unmet need for ex vivo models able to readily assess the effectiveness of immunotherapeutic treatments ...

2024

Engineered signaling centers for the spatially controlled patterning of human pluripotent stem cells

Ambroise Roger Vuaridel, Matthias Lütolf, Felix Naef, Yoji Tabata, Eric Paquet, François Réal Rivest, Andrea Manfrin

Signaling centers, localized groups of cells that secrete morphogens, play a key role in early development and organogenesis by orchestrating spatial cell fate patterning. Here we present a microfluidic approach that exposes human pluripotent stem cell (hP ...

NATURE PUBLISHING GROUP2019