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Matthias Lütolf

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Related publications (180)

Morphodynamics of human early brain organoid development

Brain organoids enable the mechanistic study of human brain development and provide opportunities to explore self-organization in unconstrained developmental systems1, 2–3. Here we establish long-term, live light-sheet microscopy on unguided brain organoid ...

Springer Science and Business Media LLC2025

Establishment of a microwell-array-based miniaturized thymic organoid model suitable for high-throughput applications

Matthias Lütolf, Tania Hübscher

T cell development depends critically on the thymic stroma—in particular, the diverse array of functionally distinct thymic epithelial cell (TEC) types. However, a robust in vitro thymus model mimicking the native thymus and compatible with medium-/high-th ...

2025

An extracellular matrix niche secreted by epithelial cells drives intestinal organoid formation

Matthias Lütolf, Antonius Chrisnandy

Cell Press - Elsevier2025

Spatiotemporal Interrogation of Single Spheroids Using Multiplexed Nanoplasmonic-fluorescence Imaging

Matthias Lütolf, Hatice Altug, Yen-Cheng Liu, Saeid Ansaryan, Luis Francisco Lorenzo Martin

Advances in organoid models, as ex vivo mini-organs, and the development of screening imaging technologies have continuously driven each other forward. A complete understanding of organoids requires detailed insights into the intertwined intraorganoid and ...

WILEY-V C H VERLAG GMBH2025

Accessible homeostatic gastric organoids reveal secondary cell type-specific host-pathogen interactions in Helicobacter pylori infections

Matthias Lütolf, Moritz Hofer, Nicolas Antoine Vincent Broguiere

Despite the high prevalence of gastric diseases like gastric cancer and peptic ulcer disease attributed to Helicobacter pylori infections, there is still only a limited understanding of the underlying mechanisms. Existing in vitro models are either two-dim ...

NATURE PORTFOLIO2025

An Integrated Transcriptomic Cell Atlas of Human Endoderm-derived Organoids

Matthias Lütolf

Human pluripotent stem cells and tissue-resident fetal and adult stem cells can generate epithelial tissues of endodermal origin in vitro that recapitulate aspects of developing and adult human physiology. Here, we integrate single-cell transcriptomes from ...

NATURE PORTFOLIO2025

Bioengineered Tubular Biliary Organoids

Matthias Lütolf, Saba Rezakhani, Mikhail Nikolaev, Bilge Sen Elçi

Liver organoids have emerged as promising in vitro models for toxicology, drug discovery, and disease modeling. However, conventional 3D epithelial organoid culture systems suffer from significant drawbacks, including limited culture duration, a nonphysiol ...

Wiley2024

Open-source microscope add-on for structured illumination microscopy

Matthias Lütolf, Theo Lasser, Aleksandra Radenovic, John McKinney, Georg Fantner, Olivier François Venzin, Mélanie Thérèse Marie Hannebelle, Tomas Lukes, Samuel Mendes Leitão, Antonius Chrisnandy, Chiara Toniolo, Andrew Charles Oates, Prabhu Prasad Swain, Nathan Cyprien Arsène Ronceray

Super-resolution techniques expand the abilities of researchers who have the knowledge and resources to either build or purchase a system. This excludes the part of the research community without these capabilities. Here we introduce the openSIM add-on to ...

Nature Portfolio2024

Collagen signaling and matrix stiffness regulate multipotency in glandular epithelial stem cells in mice

Matthias Lütolf, Saba Rezakhani, Antonius Chrisnandy

Glandular epithelia, including mammary gland (MG) and prostate, are composed of luminal and basal cells. During embryonic development, glandular epithelia arise from multipotent stem cells (SCs) that are replaced after birth by unipotent basal and unipoten ...

Nature Research2024

Mini-colons unlock tumour development outside the body

Matthias Lütolf, Luis Francisco Lorenzo Martin

In this Tools of the Trade article, Francisco Lorenzo-Mart & iacute;n and Matthias Lutolf present mini-colons as a new ex vivo cancer model that incorporates microfabrication, tissue engineering and optogenetics. ...

Nature Portfolio2024

Patterned gastrointestinal monolayers with bilateral access as observable models of parasite gut infection

Matthias Lütolf, Moritz Hofer

Organoids for modelling the physiology and pathology of gastrointestinal tissues are constrained by a poorly accessible lumen. Here we report the development and applicability of bilaterally accessible organoid-derived patterned epithelial monolayers that ...

2024

Next-generation patient models for colorectal cancer research

Matthias Lütolf, Luis Francisco Lorenzo Martin

Lab-grown 'mini-colons' enable the simulation of colorectal tumor dynamics and the high-resolution study of interactions with cells from the tumor's native microenvironment. These next-generation organoids offer a wealth of new experimental opportunities, ...

NATURE PORTFOLIO2024

Assessing the precision of morphogen gradients in neural tube development

Matthias Lütolf, Nathalie Brandenberg

Nature Portfolio2024

In vitro human cell-based models: What can they do and what are their limitations?

Matthias Lütolf

It is said that all models are wrong, but some are useful. In vitro human cell-based models are a prime example of this maxim. We asked researchers: when is your model system useful? How can it be made more useful? What are its limitations? ...

CELL PRESS2024

Thymic epithelial organoids mediate T-cell development

Matthias Lütolf, Tania Hübscher

Although the advent of organoids has opened unprecedented perspectives for basic and translational research, immune system-related organoids remain largely underdeveloped. Here, we established organoids from the thymus, the lymphoid organ responsible for T ...

2024

Probing the killing potency of tumor-infiltrating lymphocytes on microarrayed colorectal cancer tumoroids

Matthias Lütolf, Damien Saugy, Nathalie Brandenberg, François Réal Rivest, Sylke Höhnel, Krisztian Homicsko, Nicolas Antoine Vincent Broguiere, Devanjali Dutta

Immunotherapy has emerged as a new standard of care for certain cancer patients with specific cellular and molecular makeups. However, there is still an unmet need for ex vivo models able to readily assess the effectiveness of immunotherapeutic treatments ...

2024

Patient-derived mini-colons enable long-term modeling of tumor–microenvironment complexity

Matthias Lütolf, Nicolas Antoine Vincent Broguiere

Existing organoid models fall short of fully capturing the complexity of cancer because they lack sufficient multicellular diversity, tissue-level organization, biological durability and experimental flexibility. Thus, many multifactorial cancer processes, ...

2024

Spatiotemporally resolved colorectal oncogenesis in mini-colons ex vivo

Matthias Lütolf, Freddy Radtke, Tania Hübscher, Amber Dawn Bowler, Nicolas Antoine Vincent Broguiere, Luis Francisco Lorenzo Martin

Three-dimensional organoid culture technologies have revolutionized cancer research by allowing for more realistic and scalable reproductions of both tumour and microenvironmental structures 1-3 . This has enabled better modelling of low-complexity cancer ...

NATURE PORTFOLIO2024

Multimodal characterization of murine gastruloid development

Matthias Lütolf, Raphaël Ortiz, Stefano Davide Vianello, Alexandre Gauthier Aurèle Mayran

Gastruloids are 3D structures generated from pluripotent stem cells recapitulating fundamental principles of embryonic pattern formation. Using single-cell genomic analysis, we provide a resource mapping cell states and types during gastruloid development ...

CELL PRESS2023

Embryo-uterine interaction coordinates mouse embryogenesis during implantation

Matthias Lütolf, Saba Rezakhani, Mikhail Nikolaev

Embryo implantation into the uterus marks a key transition in mammalian development. In mice, implantation is mediated by the trophoblast and is accompanied by a morphological transition from the blastocyst to the egg cylinder. However, the roles of tropho ...

WILEY2023

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