Lecture

Hematopoietic Stem Cells: Clinical Applications & Assays

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In course

BIO-447: Stem cells and organoids

This course introduces the fundamentals of stem cell biology, with a particular focus on the role of stem cells during development, tissue homeostasis/regeneration and disease, and the generation of organoids from stem cells ...

Description

This lecture covers the relevance of hematopoietic stem cells (HSCs) in therapy and basic science, including their normal function, hierarchy, sources, and transplantation procedures. It delves into the clinical applications of HSC transplantation, focusing on autologous vs. allogeneic grafts, donor compatibility, key events in blood reconstitution, and the success rates and complications of HSC transplantation. The lecture also explores functional stem cell assays, the identification of HSCs in bone marrow, and the assessment of HSC function through in vivo and in vitro assays.

Instructors (4)

Matthias Lütolf

Matthias Lutolf is Full Professor at EPFL’s Institute of Bioengineering, with a cross appointment in the Institute of Chemical Sciences and Engineering. Lutolf was trained as a Materials Engineer at ETH Zurich where he also carried out his PhD studies (with Jeffrey Hubbell) that were awarded with an ETH medal. He continued his research training as a Post-Doctoral Fellow in Stem Cell Biology (with Helen Blau) at Stanford University. He has served as the Director of the Institute of Bioengineering from 2014 to 2018. Lutolf is an internationally recognized leader in the fields of stem cell bioengineering and tissue engineering. His research program uniquely combines stem cell biology with engineering principles and quantitative thinking. His team, composed of engineers, chemists, physicists, cell and developmental biologists, strives to develop technologies that have true biological and medicinal function and applicability. Lutolf’s work has led to more than 110 peer-reviewed scientific publications, many of which published in highly reputed journals, more than 25 patents, and the commercialization of several products. Current research in the Lutolf lab is focused on the bioengineering of miniature tissues, termed organoids, that are generated from self-organizing stem cells.

Freddy Radtke

Freddy Radtke obtained his Ph.D. in Molecular Biology from the University of Zürich in 1994. In 1995, he started his postdoctoral research in the laboratory of Michel Aguet at Genentech, Inc. (San Francisco, USA). In 1997, he returned to Switzerland with Michel Aguet and finished his postdoctoral fellowship at the Swiss Institute for Experimental Cancer Research (ISREC) in Lausanne. From 1999‑2005, he was a group leader and Associate Member at the Ludwig Institute for Cancer Research. Freddy Radtke then joined ISREC in January 2006 as a senior scientist and in July 2006, he was appointed Associate Professor at the EPFL School of Life Sciences

David Michael Suter

Teaching & PhD PhD Students Ekaterina Lyublinskaya, Yuliia Romaniuk, Joanna Agnieszka Dembska, Solomon Nshemereirwe Past EPFL PhD Students Onur Tidin, Aleksandra Mandic, Daniel Strebinger, Andrea Brigitta Alber, Mahé Raccaud, Torgil Elias Friman, Shoujie Sun, Silja Michelle Placzek, Romane Mizeret, Benjamin Martin, Sim Sakong, Stella Katharina Krämer Past EPFL PhD Students as codirector Damien Lionel Nicolas, Isabelle Chareyron, Christopher Tadhg James Wall, Kolly Dominique Courses Immunology BI

Official source

Ontological neighbourhood

Health sciences

Immunology: Immune system

Biological engineering

Biomedical engineering: Tissue engineering

Related concepts (32)

Adult stem cell

Adult stem cells are undifferentiated cells, found throughout the body after development, that multiply by cell division to replenish dying cells and regenerate damaged tissues. Also known as somatic stem cells (from Greek σωματικóς, meaning of the body), they can be found in juvenile, adult animals, and humans, unlike embryonic stem cells. Scientific interest in adult stem cells is centered around two main characteristics.

Stem-cell therapy

Stem-cell therapy is the use of stem cells to treat or prevent a disease or condition. , the only established therapy using stem cells is hematopoietic stem cell transplantation. This usually takes the form of a bone-marrow transplantation, but the cells can also be derived from umbilical cord blood. Research is underway to develop various sources for stem cells as well as to apply stem-cell treatments for neurodegenerative diseases and conditions such as diabetes and heart disease.

Stem-cell niche

Stem-cell niche refers to a microenvironment, within the specific anatomic location where stem cells are found, which interacts with stem cells to regulate cell fate. The word 'niche' can be in reference to the in vivo or in vitro stem-cell microenvironment. During embryonic development, various niche factors act on embryonic stem cells to alter gene expression, and induce their proliferation or differentiation for the development of the fetus.

Bone marrow

Bone marrow is a semi-solid tissue found within the spongy (also known as cancellous) portions of bones. In birds and mammals, bone marrow is the primary site of new blood cell production (or haematopoiesis). It is composed of hematopoietic cells, marrow adipose tissue, and supportive stromal cells. In adult humans, bone marrow is primarily located in the ribs, vertebrae, sternum, and bones of the pelvis. Bone marrow comprises approximately 5% of total body mass in healthy adult humans, such that a man weighing 73 kg (161 lbs) will have around 3.

Bone marrow examination

Bone marrow examination refers to the pathologic analysis of samples of bone marrow obtained by bone marrow biopsy (often called trephine biopsy) and bone marrow aspiration. Bone marrow examination is used in the diagnosis of a number of conditions, including leukemia, multiple myeloma, lymphoma, anemia, and pancytopenia. The bone marrow produces the cellular elements of the blood, including platelets, red blood cells and white blood cells.

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