Lecture

Scanning Transmission X-ray Microscopy: Techniques and Applications

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Description

This lecture covers the principles and applications of Scanning Transmission X-ray Microscopy (STXM), focusing on the water window region and its experimental details. STXM allows for microspectroscopy with a tight focus, raster scanning, and combination with XRF and Auger. It is commonly used in soft X-ray regime for polymer technologies, biological imaging, and soil science. The water window region, transparent below the oxygen K-edge, enables the study of organic compounds and biologically relevant elements. XANES spectra provide valuable information for distinguishing DNA, RNA, histone, and protein. Experimental details include synchrotron-only techniques, vacuum requirements, and carbon contamination mitigation strategies.

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Related concepts (32)

Soil science

Soil science is the study of soil as a natural resource on the surface of the Earth including soil formation, classification and mapping; physical, chemical, biological, and fertility properties of soils; and these properties in relation to the use and management of soils. Sometimes terms which refer to branches of soil science, such as pedology (formation, chemistry, morphology, and classification of soil) and edaphology (how soils interact with living things, especially plants), are used as if synonymous with soil science.

Histone

In biology, histones are highly basic proteins abundant in lysine and arginine residues that are found in eukaryotic cell nuclei. They act as spools around which DNA winds to create structural units called nucleosomes. Nucleosomes in turn are wrapped into 30-nanometer fibers that form tightly packed chromatin. Histones prevent DNA from becoming tangled and protect it from DNA damage. In addition, histones play important roles in gene regulation and DNA replication. Without histones, unwound DNA in chromosomes would be very long.

Agricultural soil science

Agricultural soil science is a branch of soil science that deals with the study of edaphic conditions as they relate to the production of food and fiber. In this context, it is also a constituent of the field of agronomy and is thus also described as soil agronomy. Prior to the development of pedology in the 19th century, agricultural soil science (or edaphology) was the only branch of soil science. The bias of early soil science toward viewing soils only in terms of their agricultural potential continues to define the soil science profession in both academic and popular settings .

RNA

Ribonucleic acid (RNA) is a polymeric molecule that is essential for most biological functions, either by performing the function itself (Non-coding RNA) or by forming a template for production of proteins (messenger RNA). RNA and deoxyribonucleic acid (DNA) are nucleic acids. The nucleic acids constitute one of the four major macromolecules essential for all known forms of life. RNA is assembled as a chain of nucleotides.

Histone deacetylase

Histone deacetylases (, HDAC) are a class of enzymes that remove acetyl groups (O=C-CH3) from an ε-N-acetyl lysine amino acid on both histone and non-histone proteins. HDACs allow histones to wrap the DNA more tightly. This is important because DNA is wrapped around histones, and DNA expression is regulated by acetylation and de-acetylation. HDAC's action is opposite to that of histone acetyltransferase. HDAC proteins are now also called lysine deacetylases (KDAC), to describe their function rather than their target, which also includes non-histone proteins.

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Related concepts (32)

Soil science

Histone

Agricultural soil science

RNA

Histone deacetylase