Lecture

Tessellation: SMLM Data Analysis

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Description

This lecture covers the principles of tessellation for single molecule localization microscopy (SMLM) data analysis, focusing on Voronoi tessellation as a space subdividing technique and colocalization analysis using Pearson and Manders' coefficients. The instructor presents applications in various fields, such as synaptic proteins and plant biology, highlighting the benefits and limitations of the method. Pros include robustness to localization density and multiscale segmentation, with fast 2D and 3D Voronoi generation. Cons involve challenges with non-homogeneous density, addressed through region of interest selection. The lecture also discusses the integration of state-of-the-art quantification methods and future developments in 3D analysis tools.

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Related concepts (32)

Voronoi diagram

In mathematics, a Voronoi diagram is a partition of a plane into regions close to each of a given set of objects. In the simplest case, these objects are just finitely many points in the plane (called seeds, sites, or generators). For each seed there is a corresponding region, called a Voronoi cell, consisting of all points of the plane closer to that seed than to any other. The Voronoi diagram of a set of points is dual to that set's Delaunay triangulation.

Botany

Botany, also called plant science (or plant sciences), plant biology or phytology, is the science of plant life and a branch of biology. A botanist, plant scientist or phytologist is a scientist who specialises in this field. The term "botany" comes from the Ancient Greek word βοτάνη (botanē) meaning "pasture", "herbs" "grass", or "fodder"; βοτάνη is in turn derived from βόσκειν (), "to feed" or "to graze".

Biology

Biology is the scientific study of life. It is a natural science with a broad scope but has several unifying themes that tie it together as a single, coherent field. For instance, all organisms are made up of cells that process hereditary information encoded in genes, which can be transmitted to future generations. Another major theme is evolution, which explains the unity and diversity of life. Energy processing is also important to life as it allows organisms to move, grow, and reproduce.

Synaptic vesicle

In a neuron, synaptic vesicles (or neurotransmitter vesicles) store various neurotransmitters that are released at the synapse. The release is regulated by a voltage-dependent calcium channel. Vesicles are essential for propagating nerve impulses between neurons and are constantly recreated by the cell. The area in the axon that holds groups of vesicles is an axon terminal or "terminal bouton". Up to 130 vesicles can be released per bouton over a ten-minute period of stimulation at 0.2 Hz.

Habit (biology)

Habit, equivalent to habitus in some applications in biology, refers variously to aspects of behaviour or structure, as follows: In zoology (particularly in ethology), habit usually refers to aspects of more or less predictable behaviour, instinctive or otherwise, though it also has broader application. Habitus refers to the characteristic form or morphology of a species. In botany, habit is the characteristic form in which a given species of plant grows (see plant habit).

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