Gel electrophoresisGel electrophoresis is a method for separation and analysis of biomacromolecules (DNA, RNA, proteins, etc.) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.
Gel electrophoresis of proteinsProtein electrophoresis is a method for analysing the proteins in a fluid or an extract. The electrophoresis may be performed with a small volume of sample in a number of alternative ways with or without a supporting medium, namely agarose or polyacrylamide. Variants of gel electrophoresis include SDS-PAGE, free-flow electrophoresis, electrofocusing, isotachophoresis, affinity electrophoresis, immunoelectrophoresis, counterelectrophoresis, and capillary electrophoresis. Each variant has many subtypes with individual advantages and limitations.
Polyacrylamide gel electrophoresisPolyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility. Electrophoretic mobility is a function of the length, conformation, and charge of the molecule. Polyacrylamide gel electrophoresis is a powerful tool used to analyze RNA samples.
Two-dimensional gel electrophoresisTwo-dimensional gel electrophoresis, abbreviated as 2-DE or 2-D electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins. Mixtures of proteins are separated by two properties in two dimensions on 2D gels. 2-DE was first independently introduced by O'Farrell and Klose in 1975. 2-D electrophoresis begins with electrophoresis in the first dimension and then separates the molecules perpendicularly from the first to create an electropherogram in the second dimension.
3D printing3D printing or additive manufacturing is the construction of a three-dimensional object from a CAD model or a digital 3D model. It can be done in a variety of processes in which material is deposited, joined or solidified under computer control, with material being added together (such as plastics, liquids or powder grains being fused), typically layer by layer. In the 1980s, 3D printing techniques were considered suitable only for the production of functional or aesthetic prototypes, and a more appropriate term for it at the time was rapid prototyping.
Agarose gel electrophoresisAgarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, genetics, and clinical chemistry to separate a mixed population of macromolecules such as DNA or proteins in a matrix of agarose, one of the two main components of agar. The proteins may be separated by charge and/or size (isoelectric focusing agarose electrophoresis is essentially size independent), and the DNA and RNA fragments by length.
Serum protein electrophoresisSerum protein electrophoresis (SPEP or SPE) is a laboratory test that examines specific proteins in the blood called globulins. The most common indications for a serum protein electrophoresis test are to diagnose or monitor multiple myeloma, a monoclonal gammopathy of uncertain significance (MGUS), or further investigate a discrepancy between a low albumin and a relatively high total protein. Unexplained bone pain, anemia, proteinuria, chronic kidney disease, and hypercalcemia are also signs of multiple myeloma, and indications for SPE.
Network layerIn the seven-layer OSI model of computer networking, the network layer is layer 3. The network layer is responsible for packet forwarding including routing through intermediate routers. The network layer provides the means of transferring variable-length network packets from a source to a destination host via one or more networks. Within the service layering semantics of the OSI (Open Systems Interconnection) network architecture, the network layer responds to service requests from the transport layer and issues service requests to the data link layer.
ManufacturingManufacturing is the creation or production of goods with the help of equipment, labor, machines, tools, and chemical or biological processing or formulation. It is the essence of the secondary sector of the economy. The term may refer to a range of human activity, from handicraft to high-tech, but it is most commonly applied to industrial design, in which raw materials from the primary sector are transformed into finished goods on a large scale.
StereolithographyStereolithography (SLA or SL; also known as vat photopolymerisation, optical fabrication, photo-solidification, or resin printing) is a form of 3D printing technology used for creating models, prototypes, patterns, and production parts in a layer by layer fashion using photochemical processes by which light causes chemical monomers and oligomers to cross-link together to form polymers. Those polymers then make up the body of a three-dimensional solid.
Session layerIn the seven-layer OSI model of computer networking, the session layer is layer 5. The session layer provides the mechanism for opening, closing and managing a session between end-user application processes, i.e., a semi-permanent dialogue. Communication sessions consist of requests and responses that occur between applications. Session-layer services are commonly used in application environments that make use of remote procedure calls (RPCs). An example of a session-layer protocol is the OSI protocol suite session-layer protocol, also known as X.
Presentation layerIn the seven-layer OSI model of computer networking, the presentation layer is layer 6 and serves as the data translator for the network. It is sometimes called the syntax layer. Within the service layering semantics of the OSI network architecture, the presentation layer responds to service requests from the application layer and issues service requests to the session layer through a unique presentation service access point (PSAP).
Manufacturing engineeringManufacturing engineering or production engineering is a branch of professional engineering that shares many common concepts and ideas with other fields of engineering such as mechanical, chemical, electrical, and industrial engineering. Manufacturing engineering requires the ability to plan the practices of manufacturing; to research and to develop tools, processes, machines and equipment; and to integrate the facilities and systems for producing quality products with the optimum expenditure of capital.
Transport layerIn computer networking, the transport layer is a conceptual division of methods in the layered architecture of protocols in the network stack in the Internet protocol suite and the OSI model. The protocols of this layer provide end-to-end communication services for applications. It provides services such as connection-oriented communication, reliability, flow control, and multiplexing. The details of implementation and semantics of the transport layer of the Internet protocol suite, which is the foundation of the Internet, and the OSI model of general networking are different.
Link layerIn computer networking, the link layer is the lowest layer in the Internet protocol suite, the networking architecture of the Internet. The link layer is the group of methods and communications protocols confined to the link that a host is physically connected to. The link is the physical and logical network component used to interconnect hosts or nodes in the network and a link protocol is a suite of methods and standards that operate only between adjacent network nodes of a network segment.
Computer-aided manufacturingComputer-aided manufacturing (CAM) also known as computer-aided modeling or computer-aided machining is the use of software to control machine tools in the manufacturing of work pieces. This is not the only definition for CAM, but it is the most common. It may also refer to the use of a computer to assist in all operations of a manufacturing plant, including planning, management, transportation and storage.
WeekA week is a unit of time equal to seven days. It is the standard time period used for short cycles of days in most parts of the world. The days are often used to indicate common work days and rest days, as well as days of worship. Weeks are often mapped against yearly calendars, but are typically not the basis for them, as weeks are not based on astronomy. The modern seven-day week can be traced back to the Babylonians, who used it within their calendar.
Gijs BlomGijsbrecht Thijmen Matthias "Gijs" Blom (born 2 January 1997) is a Dutch actor. He starred in the films Jongens (2014) and The Forgotten Battle (2020). He received a Daytime Emmy nomination for his performance in the Netflix fantasy series The Letter for the King (2020). Blom is from Amsterdam. He is the son of the stage actress Marloes van den Heuvel. He graduated with a degree in theatre from Amsterdam University of the Arts' Academy of Theatre and Dance in 2021. From 2007 to 2009, Blom starred in the musical Ciske de Rat.
Antje von GraevenitzAntje (-Maria) von Graevenitz, born Ludwig (26 August 1940, Hamburg) is a German art historian, art critic, educator and author. Since 1970, Antje von Graevenitz has lived in Amsterdam. As a professor of general art history, specialized in the history of 20th- and 21st-century art, she taught at the University of Amsterdam (as a lecturer 1977-1989) and Cologne (as a professor 1989-2005). In her research she is mainly focused on ephemeral art (performances and Fluxus) and interdisciplinary and anthropological topics (art in relation to rites of passage, philosophy, music, theatre or dance).
HitRadio VeronicaHitRadio Veronica was a Dutch commercial radio station that was created when the public broadcaster Veronica Omroep Organisatie left the public system in August 1995, creating the Holland Media Groep with CLT (RTL). Before that Veronica bought the station Holland FM and rebranded it into Hitradio Holland FM and later Hitradio 1224. Most of known Veronica DJs switched to Hitradio 1224. HitRadio Veronica has broadcast on 828 kHz and 1224 kHz from 1995 till 1996, the slogan was De hits van gisteren en het beste van nu.
