Lecture

Inhibitors Analysis: Amyloride & Wabahine

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In courses (2)

BIO-378: Physiology lab I

Le TP de physiologie introduit les approches expérimentales du domaine biomédical, avec les montages de mesure, les capteurs, le conditionnement des signaux, l'acquisition et traitement de données. Les résultats physiologiques finaux illustrent le contenu ...

BIO-379: Physiology lab II

Description

This lecture delves into the analysis of two inhibitors, amyloride and wabahine, used to study the transport of sodium. By examining the effects of these inhibitors on sodium transport, the instructor explains the intricacies of diffusion, affinity, and the challenges in removing inhibitors. The lecture highlights the differences in action time between amyloride and wabahine, shedding light on the complexities of inhibitor kinetics and their impact on ion transport processes.

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Instructor

André Pexieder

Teaching & PhD Courses Physiology lab I BIO-378 The physiology laboratory introduce the experimental methods in the biomedical field, including experimental set-ups, transducers, signal conditioning, data acquisition and processing.The physiological end results illustrate the contents of the Physiologie par systèmes course. Physiology lab II BIO-379 The physiology laboratory introduce the experimental methods in the biomedical field, including experimental set-ups, transducer

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Related concepts (19)

Ion channel

Ion channels are pore-forming membrane proteins that allow ions to pass through the channel pore. Their functions include establishing a resting membrane potential, shaping action potentials and other electrical signals by gating the flow of ions across the cell membrane, controlling the flow of ions across secretory and epithelial cells, and regulating cell volume. Ion channels are present in the membranes of all cells. Ion channels are one of the two classes of ionophoric proteins, the other being ion transporters.

Voltage-gated ion channel

Voltage-gated ion channels are a class of transmembrane proteins that form ion channels that are activated by changes in the electrical membrane potential near the channel. The membrane potential alters the conformation of the channel proteins, regulating their opening and closing. Cell membranes are generally impermeable to ions, thus they must diffuse through the membrane through transmembrane protein channels. They have a crucial role in excitable cells such as neuronal and muscle tissues, allowing a rapid and co-ordinated depolarization in response to triggering voltage change.

Diffusion

Diffusion is the net movement of anything (for example, atoms, ions, molecules, energy) generally from a region of higher concentration to a region of lower concentration. Diffusion is driven by a gradient in Gibbs free energy or chemical potential. It is possible to diffuse "uphill" from a region of lower concentration to a region of higher concentration, like in spinodal decomposition. Diffusion is a stochastic process due to the inherent randomness of the diffusing entity and can be used to model many real-life stochastic scenarios.

Sodium channel

Sodium channels are integral membrane proteins that form ion channels, conducting sodium ions (Na+) through a cell's membrane. They belong to the superfamily of cation channels. They are classified into 2 types: In excitable cells such as neurons, myocytes, and certain types of glia, sodium channels are responsible for the rising phase of action potentials. These channels go through three different states called resting, active and inactive states.

Potassium channel

Potassium channels are the most widely distributed type of ion channel found in virtually all organisms. They form potassium-selective pores that span cell membranes. Potassium channels are found in most cell types and control a wide variety of cell functions. Potassium channels function to conduct potassium ions down their electrochemical gradient, doing so both rapidly (up to the diffusion rate of K+ ions in bulk water) and selectively (excluding, most notably, sodium despite the sub-angstrom difference in ionic radius).

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