Publications related to Dark-field microscopy

Virtual High-Framerate Microscopy of the Beating Heart via Sorting of Still Images

Michael Stefan Daniel Liebling, Olivia Mariani

Microscopy of the beating heart in embryos provides key insights for the study of its development. However, achieving a sufficiently high framerate is difficult with conventional cameras. Here, we present a method to reconstruct an image sequence covering ...

Idiap2019

Endogenous SHG and 2PEF coherence imaging of substructures in neurons in 3D

Sylvie Roke, Carlos Alberto Macias Romero, Marie Eve Pascale Didier, Claire Jacqueline Léa Teulon

Neuronal morphology, long-distance transport and signalling critically depend on the organization of microtubules in the cytoskeleton. Second harmonic generation (SHG) imaging has been recognized as a potentially powerful tool for in situ label-free neuroi ...

2019

Simultaneous temporal superresolution and denoising for cardiac fluorescence microscopy

Michael Stefan Daniel Liebling

Due to low light emission of fluorescent samples, live fluorescence microscopy imposes a tradeoff between spatiotemporal resolution and signal-to-noise ratio. This can result in images and videos containing motion blur or Poisson-type shot noise, depending ...

2016

Intensity-Based Point-Spread-Function-Aware Registration for Multi-View Applications in Optical Microscopy

We present an algorithm to spatially register two volumetric datasets related via a rigid-body transform and degraded by an anisotropic point-spread-function (PSF). Registration is necessary, for example, when fusing data in multi-view microscopy. Automatic algorithms that only rely on maximizing pixel similarity, without accounting for the anisotropic image formation process, provide poor results in such applications. We propose to solve this problem by re-blurring the reference and test data with transfor

2015

Fast detection of single nanoparticles in a microfluidic channel by a microlens array in combination with a conventional optical microscope

Martinus Adela Maria Gijs

We present the use of a microlens array in combination with a conventional optical microscope set-up for the detection of single nanoparticles (NPs) in fluid medium. Optically transparent dielectric microspheres are patterned in a microfabricated well array template and used as microlenses focusing the light originating from a microscope objective into so-called photonic nanojets that expose the medium within the microfluidic channel. When the NPs pass the nanojets, the detection signal is highly enhanced,

2015

Challenges and Rewards of the Electrosynthesis of Macroscopic Aligned Carbon Nanotube Array/Conducting Polymer Hybrid Assemblies

László Forró, Endre Horvath, Andrea Pisoni, Péter Krisztián Matus

Hybrid assemblies based on conducting polymers and carbon nanomaterials with organized nanoscale structure are excellent candidates for various application schemes ranging from thermal management to electrochemical energy conversion and storage. In the cas ...

Wiley-Blackwell2015

Dark-field microscopy

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