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Concept

Live-cell imaging

Related publications (28)

Stressed Mycobacteria Use the Chaperone ClpB to Sequester Irreversibly Oxidized Proteins Asymmetrically Within and Between Cells

Michaël Unser, John McKinney, Neeraj Dhar, Olivia Mariani

Mycobacterium tuberculosis (Mtb) defends itself against host immunity and chemotherapy at several levels, including the repair or degradation of irreversibly oxidized proteins (IOPs). To investigate how Mtb deals with IOPs that can neither be repaired nor ...

Cell Press2015

Tracking Interacting Objects in Image Sequences

Xinchao Wang

Object tracking in image sequences is a key challenge in computer vision. Its goal is to follow objects that move or evolve over time while preserving the identity of each object. However, most existing approaches focus on one class of objects and model on ...

EPFL2015

BactImAS: a platform for processing and analysis of bacterial time-lapse microscopy movies

Zeljka Maglica

Background: The software available to date for analyzing image sequences from time-lapse microscopy works only for certain bacteria and under limited conditions. These programs, mostly MATLAB-based, fail for microbes with irregular shape, indistinct cell d ...

Biomed Central Ltd2014

Robust synchronization of coupled circadian and cell cycle oscillators in single mammalian cells

Felix Naef, Kyle Gustafson, Cédric Gobet, Jonathan Bieler, Rosamaria Cannavo

Circadian cycles and cell cycles are two fundamental periodic processes with a period in the range of 1 day. Consequently, coupling between such cycles can lead to synchronization. Here, we estimated the mutual interactions between the two oscillators by t ...

Nature / European Molecular Biology Organization2014

In vitro Fate Mapping of Single Hematopoietic Stem Cells

Aline Roch

The in vitro expansion of hematopoietic stem cells (HSC) for clinical applications is hampered by a rapid loss of HSC blood reconstitution capability in culture. While these rare cells can be stimulated to massively proliferate, cell divisions mostly resul ...

EPFL2014

Delayed bactericidal response of Mycobacterium tuberculosis to bedaquiline involves remodelling of bacterial metabolism

John McKinney, Neeraj Dhar, Muhittin Emre Özdemir

Bedaquiline (BDQ), an ATP synthase inhibitor, is the first drug to be approved for treatment of multidrug-resistant tuberculosis in decades. Though BDQ has shown excellent efficacy in clinical trials, its early bactericidal activity during the first week o ...

Nature Publishing Group2014

Marker-free phase nanoscopy

Pierre Magistretti, Pierre Marquet, Christian Depeursinge, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Muhammed Fatih Toy, Yann Jérôme Michel Pascal Cotte

We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured us ...

Nature Publishing Group2013

Dynamic Persistence of Antibiotic-Stressed Mycobacteria

John McKinney, Neeraj Dhar, François Signorino-Gelo, Katrin Esther Schneider

Exposure of an isogenic bacterial population to a cidal antibiotic typically fails to eliminate a small fraction of refractory cells. Historically, fractional killing has been attributed to infrequently dividing or nondividing "persisters." Using microflui ...

American Association for the Advancement of Science2013