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Concept

Bioluminescence

Related publications (27)

Hyperthermic intrathoracic chemotherapy to control malignant pleural mesothelioma

Yameng Hao

OBJECTIVE: Malignant pleural mesothelioma (MPM) is a rare disease that has a poor response to conventional therapy. Hyperthermic intrathoracic chemotherapy (HITOC), a treatment combining fever-range hyperthermia with intrapleural cisplatin chemotherapy, ha ...

EPFL2023

Excess labile carbon promotes diazotroph abundance in heat-stressed octocorals

Claudia Isabella Pogoreutz

Nitrogen limitation is the foundation of stable coral-algal symbioses. Diazotrophs, prokaryotes capable of fixing N-2 into ammonia, support the productivity of corals in oligotrophic waters, but could contribute to the destabilization of holobiont function ...

ROYAL SOC2023

Bioluminescent compositions and uses thereof

Elena Goun, Pavlo Khodakivskyi, Aleksey Yevtodiyenko, Hacer Karatas, Manuel Simon Drefahl

Compositions and methods for non-invasively monitoring the intracellular processing of a biomolecule are provided. The compositions have a novel thio-luciferin moiety which can be conjugated to the biomolecule directly or via a linker such as disulfide bon ...

2021

Influence of Oxidative Stress on Time-Resolved Oxygen Detection by [Ru(Phen)3]2+ In Vivo and In Vitro

Georges Wagnières, Veronika Huntosova

Detection of tissue and cell oxygenation is of high importance in fundamental biological and in many medical applications, particularly for monitoring dysfunction in the early stages of cancer. Measurements of the luminescence lifetimes of molecular probes ...

2021

Luminescent lanthanide nanocomposites in thermometry: Chemistry of dopant ions and host matrices

Mohammad Khaja Nazeeruddin

Recently, there has been observed huge progress in the advancement of remote optical nanothermometry, as it plays a vital role in remote, noninvasive, and remote temperature sensing, which is especially important for various bio-applications, e.g., in ther ...

ELSEVIER SCIENCE SA2021

Development of novel tools for imaging of metabolic fluxes in vivo

Tamara Maric

Bioluminescent imaging is a powerful technique that enables imaging in living organisms with high sensitivity, low background signal, low cost and without the need for radioactivity. The emitted photons are produced in the oxidation reaction of luciferin c ...

EPFL2020

Shear Stress-Responsive Polymersome Nanoreactors Inspired by the Marine Bioluminescence of Dinoflagellates

Esther Amstad, Antoine Claude Jean Vian

Some marine plankton called dinoflagellates emit light in response to the movement of surrounding water, resulting in a phenomenon called milky seas or sea sparkle. The underlying concept, a shear-stress induced permeabilisation of biocatalytic reaction co ...

Wiley-VCH Verlag GmbH2021

A general framework for non-exponential delayed fluorescence and phosphorescence decay analysis, illustrated on Protoporphyrin IX

Georges Wagnières, Jaroslava Joniová, Emmanuel Louis Arthur Gerelli, Aurelien Stephen Arnaud Gregor

Delayed fluorescence (DF) is a long-lived luminescence process used in a variety of applications ranging from oxygen sensing in biological tissues to organic Light Emitting Diodes. In common cases, DF results from the deexcitation of the first excited trip ...

2020

Tracking the cargo of extracellular symbionts into host tissues with correlated electron microscopy and nanoscale secondary ion mass spectrometry imaging

Anders Meibom, Stéphane Laurent Escrig, Stéphanie Kéren Cohen

Extracellular bacterial symbionts communicate biochemically with their hosts to establish niches that foster the partnership. Using quantitative ion microprobe isotopic imaging (nanoscale secondary ion mass spectrometry [NanoSIMS]), we surveyed localizatio ...

WILEY2020

Insights into flagellar function and mechanism from the squid-vibrio symbiosis

Stéphanie Kéren Cohen

Flagella are essential and multifunctional nanomachines that not only move symbionts towards their tissue colonization site, but also play multiple roles in communicating with the host. Thus, untangling the activities of flagella in reaching, interacting, ...

2019

A biosensor for measuring NAD(+) levels at the point of care

Johan Auwerx, Kai Johnsson, Luc Patiny, Elena Katsyuba, Lin Xue, Qiuliyang Yu

The cellular level of nicotinamide adenine dinucleotide (NAD(+)), through its different functions, affects cellular metabolism and signalling(1-3). A decrease in the NAD(+) content has been associated with various pathologies and physiological aging(4,5), ...

NATURE PUBLISHING GROUP2019

Analysis of photosensor frontends for biomedical applications: a focus on noise

Paul Stanislas Gosselin

Photosensing has proven to be highly useful in biomedical research and medical care. Being able to detect and quantify the light emitted by fluorescent or bioluminescent reporters has significantly participated in the development of new cancer treatments a ...

EPFL2019

Innovative Tools and in vivo Methods for Tuberculosis Drug Discovery and Development

Raphael Christopher Sommer

Worldwide, tuberculosis (TB) is the leading cause of death due to a single infectious agent, claiming 1.6 million human lives and causing >10 million new cases in 2017 alone, mostly in low-income regions. The intracellular pathogen Mycobacterium tuberculos ...

EPFL2019

Bioorthogonal chemistry in bioluminescence imaging

Elena Goun, Aurélien Godinat, Arkadiy Bazhin

Bioorthogonal chemistry has developed significant over the past few decades, to the particular benefit of molecular imaging. Bioluminescence imaging (BLI) along with other imaging modalities have significantly benefitted from this chemistry. Here, we revie ...

ELSEVIER SCI LTD2018

Portable bioluminescence systems and methods for in vivo monitoring of molecular and metabolic events in animals

Elena Goun, Aurélien Godinat, Aleksey Yevtodiyenko

A system for monitoring biological processes in vivo is provided. The system comprises an implantable luciferase biosensor comprising luciferase in a biocompatible matrix and a caged luciferin probe. The caged luciferin probe can be administered to a livin ...

2018

Bacterial type VI secretion system facilitates niche domination

Melanie Blokesch, Natalia Carolina Drebes Dorr

Over the last few decades humanity has experienced a series of tremendous technological advances, especially in the realm of basic science. A plethora of new approaches has allowed us to appreciate life in previously unimaginable detail, prompting the real ...

2018

In Vivo Molecular Bioluminescence Imaging: New Tools and Applications

Elena Goun, Hacer Karatas

in vivo bioluminescence imaging (BLi) is an optical molecular imaging technique used to visualize molecular and cellular processes in health and diseases and to follow the fate of cells with high sensitivity using luciferase-based gene reporters. The high ...

Elsevier2017

Visualizing chemical transport from extracellular symbionts into squid host tissues using NanoSIMS

Stéphanie Kéren Cohen

Chemical communication is the basis of host-microbe interaction. Insights into this communication will provide a deeper understanding of the mechanisms that govern these complex associations, in beneficial as well as pathogenic contexts. In this dissertati ...

EPFL2017

The Split Luciferin Reaction : From Bioorthogonal Chemistry to Bioluminescence Imaging

Aurélien Godinat

Studying biological processes on the level of live cells with the help of biocompatible reac-tions has tremendously advanced our understanding of basic biology. However, the great complexity of many human pathologies such as cancer, diabetes and neurodegen ...

EPFL2017

Upconversion fluorescence imaging of HeLa cells using ROS generating SiO2-coated lanthanide-doped NaYF4 nanoconstructs

Andrzej Sienkiewicz

Inorganic nanomaterials able to generate reactive oxygen species (ROS) are promising components for modern medical applications. Activated by near-infrared light, up-converting beta-NaYF4 doped with Er3+ - Yb3+ and Tm3+- Yb3+ pair ions nanoparticles (UCNPs ...

2017