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Publication

Multiplexed surface micropatterning of proteins with a pressure-modulated microfluidic button-membrane

Sebastian Maerkl
2013
Journal Articles

Résumé

We report the flow-based in situ patterning of multiple proteins in microfluidic channels by simply tuning the actuation pressure of a microfluidic button-membrane. We show that a single button-membrane can pattern several concentric protein annuli, and we apply this patterning approach to a multiplexed immunoassay.

Source officielle

Sebastian Maerkl
2013
Journal Articles

Résumé

Source officielle

Concepts associés (14)

Microfluidique

La microfluidique est la science et la technique des systèmes manipulant des fluides et dont au moins l'une des dimensions caractéristiques est de l'ordre du micromètre. George Whitesides définit la microfluidique comme « la science et la technologie des systèmes qui manipulent de petits volumes de fluides ( à ), en utilisant des canaux de la dimension de quelques dizaines de micromètres ». Selon Patrick Tabeling, Tabeling précise qu'il entend essentiellement par « nouvelles techniques » la microfabrication héritée de la micro-électronique.

Droplet-based microfluidics

Droplet-based microfluidics manipulate discrete volumes of fluids in immiscible phases with low Reynolds number and laminar flow regimes. Interest in droplet-based microfluidics systems has been growing substantially in past decades. Microdroplets offer the feasibility of handling miniature volumes (μl to fl) of fluids conveniently, provide better mixing, encapsulation, sorting, sensing and are suitable for high throughput experiments.

Paper-based microfluidics

Paper-based microfluidics are microfluidic devices that consist of a series of hydrophilic cellulose or nitrocellulose fibers that transport fluid from an inlet through the porous medium to a desired outlet or region of the device, by means of capillary action. This technology builds on the conventional lateral flow test which is capable of detecting many infectious agents and chemical contaminants. The main advantage of this is that it is largely a passively controlled device unlike more complex microfluidic devices.

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Publications associées (32)

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Multiplexed surface micropatterning of proteins with a pressure-modulated microfluidic button-membrane

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