Publications associées à Component-resolved diagnostic of cow’s milk allergy by immunoaffinity capillary electrophoresis – matrix assisted laser desorption/ionization mass spectrometry

Publications associées (27)

Rational Design of Chemically Controlled Antibodies and Protein Therapeutics

Bruno Emanuel Ferreira De Sousa Correia, Li Tang, Pablo Gainza Cirauqui, Sandrine Madeleine Suzanne Georgeon, Stéphane Rosset, Leo Scheller, Anthony Marchand, Sailan Shui, Lucia Bonati

Protein-based therapeutics, such as monoclonal antibodies and cytokines, are important therapies for various pathophysiological conditions such as oncology, autoimmune disorders, and viral infections. However, the wide application of such protein therapeut ...

2023

Targeting molecular surfaces to engineer novel protein-based immunogens and inhibitors

Sarah Wehrle

Proteins are the basic building blocks necessary for the operation and regulation of virtually all functions in living organisms. Over millions of years, evolution has created a vast repertoire of proteins finely tuned to execute their biological functions ...

EPFL2022

Systematic analysis of biomolecular binding reactions using microfluidic systems

Amir Shahein

Transcription factor binding to a single binding site on DNA and its functional consequence in a promoter context are beginning to be relatively well understood. However, binding to clusters of sites has yet to be characterized in depth, and the functional ...

EPFL2022

A high-throughput cell- and virus-free assay shows reduced neutralization of SARS-CoV-2 variants by COVID-19 convalescent plasma

Didier Trono, Priscilla Turelli, Florence Pojer, Valeria Cagno, Charlène Mireille Raymonde Raclot, Jérémy Campos

The detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific antibodies in the serum of an individual indicates previous infection or vaccination. However, it provides limited insight into the protective nature of this immune resp ...

AMER ASSOC ADVANCEMENT SCIENCE2021

A Nanoscaffolded Spike-RBD Vaccine Provides Protection against SARS-CoV-2 with Minimal Anti-Scaffold Response

Bruno Emanuel Ferreira De Sousa Correia, David Hacker, Florence Pojer, Kelvin Ka Ching Lau

The response of the adaptive immune system is augmented by multimeric presentation of a specific antigen, resembling viral particles. Several vaccines have been designed based on natural or designed protein scaffolds, which exhibited a potent adaptive immu ...

2021

Novel Analytical Methods for Allergy-Related Studies

Mikaël Frossard

Managing and treating diseases require a deep understanding of the pathologies coupled with accurate diagnostic procedures. The constant development and improvement of bioanalytical methods reflect the need of diagnosis ameliorations with respect to accura ...

EPFL2020

Milk Allergen Detection: Sensitive Label-Free Voltammetric Immunosensor Based on Electropolymerization

Paul Jean Philipp, Sandro Carrara, Tugba Kilic

We present in this work, first time in literature, a voltammetric label-free biosensor for detection of alpha lactalbumin (alpha-LB) in meal samples. Food allergy concern necessitates strict regulations in food labelling and innovative monitoring strategie ...

2020

Functional de novo Protein Design for Targeted Vaccines and Synthetic Biology Applications

Che Yang

Evolution has created, selected and evolved large repertoires of proteins that operate in various biological systems. Nowadays biotechnological needs are coming up orders of magnitude faster than proteins naturally evolve. The emergence of de novo protein ...

EPFL2020

Expanding beyond the natural protein repertoire to engineer targeted vaccines and diagnostics

Fabian Sesterhenn

Proteins have evolved over millions of years to carry out the vast majority of biological functions that are fundamental to life. Their three-dimensional structures and functions have been in the focus of biomedical research for many decades, and substanti ...

EPFL2019

Glycotargeting therapeutics

Jeffrey Alan Hubbell, David Scott Wilson

Several embodiments of the present disclosure relate to therapeutic compositions configured to target the liver of a subject and that are useful in the treatment or prevention of one or more of transplant rejection, autoimmune disease, food allergy, and im ...

2018

Introducing tolerance to foreign and partly foreign proteins by erythrocyte binding and artificial glycosylations

Kym Lorenz Brünggel

Over the last few years, protein drugs have steadily gained importance in the clinic. Compared to small molecule drugs, protein drugs are taken up by antigen presenting cells, which degrade the proteins into peptides that are then presented on major histoc ...

EPFL2018

Glycotargeting therapeutics

Jeffrey Alan Hubbell, David Scott Wilson

Several embodiments of the present disclosure relate to glycotargeting therapeutics that are useful in the treatment of transplant rejection, autoimmune disease, food allergy, and immune response against a therapeutic agent. In several embodiments, the com ...

2017

New ionization and tagging methods for mass spectrometry

Xiaoqin Zhong

Mass spectrometry (MS) is an essential detection tool in bioanalytical chemistry owing to its exceptional selectivity and sensitivity, paired with rapid analyte identification and quantification. In this thesis, two classical MS and a newly developed ioniz ...

EPFL2017

A droplet-based heterogeneous immunoassay for screening single cells secreting antigen-specific antibodies

Samin Akbari

High throughput heterogeneous immunoassays that screen antigen-specific antibody secreting cells are essential to accelerate monoclonal antibody discovery for therapeutic applications. Here, we introduce a heterogeneous single cell immunoassay based on alg ...

Royal Soc Chemistry2014

From food to physiology characterization and in vitro digestion of milk products and investigation of postprandial metabolism and inflammation after a high-fat meal intake in a human nutrition intervention study

Flurina Schwander

According to the World Health Organization worldwide obesity has more than doubled since 1980. The World health statistics 2012 report shows that one in six adults is obese, one in ten diabetic and one in three has a raised blood pressure. Overweight and o ...

EPFL2013

Total serum IgE quantification by microfluidic ELISA using magnetic beads

Hubert Girault, Anne-Laure Gassner

The present work reports on the quantification of total IgE in human serum using a microanalytical device whose fluidics is driven by gravity and capillary forces only. Thanks to the eight parallel microchannels in each microchip, calibration and sample an ...

Springer Verlag2012

Intimate gut interactions: helminths and the microbiota

Nicola Harris

Changing exposure to intestinal helminths, or alterations in our intestinal microbiome, have been independently proposed to underlie the increasing incidence of chronic inflammatory diseases including allergy, autoimmunity and inflammatory bowel disease (I ...

Inst Biochemistry & Cell Biology2016

Bioanalytical methods for food allergy diagnosis, allergen detection and new allergen discovery

Hubert Girault, Natalia Gasilova

For effective monitoring and prevention of the food allergy, one of the emerging health problems nowadays, existing diagnostic procedures and allergen detection techniques are constantly improved. Meanwhile, new methods are also developed, and more and mor ...

Future Sci Ltd2015

Cre-mediated cell ablation contests mast cell contribution in models of antibody- and T cell-mediated autoimmunity

Nicola Harris

Immunological functions of mast cells remain poorly understood. Studies in Kit mutant mice suggest key roles for mast cells in certain antibody- and T cell-mediated autoimmune diseases. However, Kit mutations affect multiple cell types of both immune and n ...

Elsevier2011

Nanoparticle conjugation enhances the immunomodulatory effects of intranasally delivered CpG in house dust mite-allergic mice

Jeffrey Alan Hubbell, Melody Swartz, Alexandre de Titta, Marie Ballester, Laura Jeanbart

An emerging strategy in preventing and treating airway allergy consists of modulating the immune response induced against allergens in the lungs. CpG oligodeoxynucleotides have been investigated in airway allergy studies, but even if promising, efficacy re ...

Nature Publishing Group2015