Double-helix enhanced axial localization in STED nanoscopy

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Organisation and dynamics of mitochondria and their RNA

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The organisation of molecules into dynamic cells, and collaboration of many of those cells over a billion years led to the evolution of human life. During the last century, biologists then began to unravel the marvels of cellular organisation with ever inc ...

EPFL2021

QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy

Arne Seitz, Antje Keppler, Andrea Bassi

A modern day light microscope has evolved from a tool devoted to making primarily empirical observations to what is now a sophisticated , quantitative device that is an integral part of both physical and life science research. Nowadays, microscopes are fou ...

WILEY2021

NTIRE 2021 Depth Guided Image Relighting Challenge

Sabine Süsstrunk, Majed El Helou, Ruofan Zhou

Image relighting is attracting increasing interest due to its various applications. From a research perspective, image relighting can be exploited to conduct both image normalization for domain adaptation, and also for data augmentation. It also has multip ...

IEEE COMPUTER SOC2021

Phase calibration of a basic bright-field microscope for 3D metrology of transparent samples at the nanoscale

Martinus Adela Maria Gijs, Daniel Migliozzi

Optical retrieval of the structure of transparent objects at the nano-scale requires adapted methods capable of probing their interaction with suitable light. Scattering events, for instance, depend on the content and the arrangement of the medium encounte ...

SPIE-INT SOC OPTICAL ENGINEERING2021

Weakly Supervised Deep Learning Methods for Biomicroscopy

Adrian Shajkofci

Optical microscopy, an invaluable tool in biology and medicine to observe and quantify cellular function, organ development, or disease mechanisms, requires constant trade-offs between spatial, temporal, and spectral resolution, invasiveness, acquisition t ...

EPFL2021

Diffraction Tomography from Single-Molecule Localization Microscopy: Numerical Feasibility

Michaël Unser, Emmanuel Emilien Louis Soubies, Thanh-An Michel Pham, Ferréol Arnaud Marie Soulez

Single-molecule localization microscopy (SMLM) is a fluorescence microscopy technique that achieves super-resolution imaging by sequentially activating and localizing random sparse subsets of fluorophores. Each activated fluorophore emits light that then s ...

IEEE2021

Single-molecule localization microscopy

Suliana Manley, Juliette Griffie

Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at the molecular scal ...

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Additive manufacturing of a precious bulk metallic glass

Roland Logé, Jamasp Jhabvala, Antonia Neels, Navid Sohrabi, Güven Kurtuldu, Annapaola Parrilli

For the first time, a high-density amorphous and crack-free bulk metallic glass (BMG) based on a precious metal (PdCuNiP) was produced via additive manufacturing (AM). Laser powder-bed fusion (LPBF) was used for the fabrication of the samples, and led to a ...

2021

Fundamental bounds on the precision of iSCAT, COBRI and dark-field microscopy for 3D localization and mass photometry

Jonathan Yuelin Dong

Interferometric imaging is an emerging technique for particle tracking and mass photometry. Mass or position are estimated from weak signals, coherently scattered from nanoparticles or single molecules, and interfered with a co-propagating reference. In th ...

2021

Correlative 3D microscopy of single cells using super-resolution and scanning ion-conductance microscopy

Aleksandra Radenovic, Georg Fantner, Vytautas Navikas, Adrien Charles-François Raymond Descloux, Samuel Mendes Leitão, Kristin Stefanie Grussmayer, Barney Frederick Drake

High-resolution live-cell imaging is necessary to study complex biological phenomena. Modern fluorescence microscopy methods are increasingly combined with complementary, label-free techniques to put the fluorescence information into the cellular context. ...

Nature Research2021

Polarization-sensitive optics diffraction tomography

Demetri Psaltis, Joowon Lim, Amirhossein Saba Shirvan

Polarization of light has been widely used as a contrast mechanism in two-dimensional (2D) microscopy and also in some three-dimensional (3D) imaging modalities. In this paper, we report the 3D tomographic reconstruction of the refractive index (RI) tensor ...

2021