Publication

Essentiality of mmpL3 and impact of its silencing on Mycobacterium tuberculosis gene expression

Publications associées (30)

Fluorescent D-Amino Acids for Super-resolution Microscopy of the Bacterial Cell Wall

Luc Reymond, Suliana Manley, Ophélie Rutschmann, Juliette Griffie, Chen Zhang, Willi Leopold Stepp, Jiangtao Qiao, Faustine Ambroisine Charline Ryckebusch

Fluorescent D-amino acids (FDAAs) have previ-ously been developed to enable in situ highlighting of locations of bacterial cell wall growth. Most bacterial cells lie at the edge of the diffraction limit of visible light; thus, resolving the precise details ...

2022

Preparation and Analysis of Bacteria Surface-Modified with Polymer Nanoparticles

Lisa Patricia Elisabeth Bader

Cell-based drug carriers present an interesting approach to improve the targeted delivery of therapeutic drugs in vivo. Specifically, bacterial cells possess interesting properties such as motility and sensing that allow some strains to selectively target ...

EPFL2022

Event-driven acquisition for content-enriched microscopy

Suliana Manley, Dora Mahecic, Juliette Griffie, Chen Zhang, Martin Weigert, Willi Leopold Stepp

Event-driven acquisition uses neural-network-based recognition of specific biological events to trigger switching between slow and fast super-resolution imaging, enriching the capture of interesting events with high spatiotemporal resolution. A common goal ...

2022

Water as a contrast agent for imaging interfacial structure and ion transport in giant vesicles

David Roesel

Hydrated lipid bilayer membranes and their asymmetry play a fundamental role in living cells by maintaining and regulating concentration gradients between cells, their environment, and their compartments. They achieve this not only through various channels ...

EPFL2022

Computational Studies of the Proton-Coupled Metal Ion Transport in the SLC11/NRAMP Family of Transporters

Maria Letizia Merlini

In the last years, it has been demonstrated a link between the overload of metal ions inside nervous system cells and the onset of severe neurodegenerative diseases. This prompted the investigation of the structural and functional properties of transporter ...

EPFL2022

Second harmonic scattering of water in a biological context

Tereza Schönfeldová

Water is one of the most abundant molecules in the universe. It forms a hydrogen bond network with unique structure and dynamics. Hydrogen bonding is highly relevant to many biological processes including membrane formation, protein folding, adsorption, an ...

EPFL2022

Live-seq enables temporal transcriptomic recording of single cells

Bart Deplancke, Pernille Yde Rainer, Riccardo Dainese, Wanze Chen, Magda Zachara, Vincent Roland Julien Gardeux, Wouter Saelens, Orane Josiane Guillaume-Gentil

Single-cell transcriptomics (scRNA-seq) has greatly advanced our ability to characterize cellular heterogeneity(1). However, scRNA-seq requires lysing cells, which impedes further molecular or functional analyses on the same cells. Here, we established Liv ...

NATURE PORTFOLIO2022

Multivalent Peptide Ligands To Probe the Chromocenter Microenvironment in Living Cells

Nora Guidotti, Maxime Nicolas Mivelaz, Beat Fierz, Pablo Rivera Fuentes, Adam Miklos Eördögh

Chromatin is spatially organized into functional states that are defined by both the presence of specific histone post-translational modifications (PTMs) and a defined set of chromatin-associated “reader” proteins. Different models for the underlying mecha ...

2022

Colon tumour cell death causes mTOR dependence by paracrine P2X4 stimulation

Andrea Ablasser

Solid cancers exhibit a dynamic balance between cell death and proliferation ensuring continuous tumour maintenance and growth(1,2). Increasing evidence links enhanced cancer cell apoptosis to paracrine activation of cells in the tumour microenvironment in ...

NATURE PORTFOLIO2022

Method and system for determining intracellular mediators' activity

Horst Vogel, Philippe Renaud, Thamani Dahoun, Margaux Catherine Marie Duchamp, Marc Brugarolas Campillos

A method for determining the presence of a molecule coupled to the cytoplasmic side of a cellular membrane is disclosed. The method is implemented in a microfluidic setting and is particularly suitable for determining the presence and/or the activity of a ...

2022