Publications associées à Multiwavelength High Resolution Interference Microscopy (HRIM) for the characterization of small size microlenes

Compact and effective photon-resolved image scanning microscope

Fluorescence confocal laser-scanning microscopy (LSM) is one of the most popular tools for life science research. This popularity is expected to grow thanks to single-photon array detectors tailored for LSM. These detectors offer unique single-photon spati ...

Spie-Soc Photo-Optical Instrumentation Engineers2024

Ghost imaging using two SPAD array detectors: a parameter study towards the realization of a 3D quantum microscope

Claudio Bruschini, Edoardo Charbon, Paul Mos, Yang Lin

Quantum ghost imaging can be an important tool in making optical measurements. One of the most useful aspects of ghost imaging is the unique ability to correlate two sets of independently collected information. We aim to use the principles of ghost imaging ...

Spie-Int Soc Optical Engineering2024

Label-Free Techniques for Probing Biomolecular Condensates

Aleksandra Radenovic, Khalid Ashraf Mohie Ibrahim, Helena Miljkovic, Wayne Yang Wen Wei, Akhil Sai Naidu

Biomolecular condensates play important roles in a wide array of fundamental biological processes, such as cellular compartmentalization, cellular regulation, and other biochemical reactions. Since their discovery and first observations, an extensive and e ...

Amer Chemical Soc2024

Fluorescence microscopy: A statistics-optics perspective

Aleksandra Radenovic

Fundamental properties of light unavoidably impose features on images collected using fluorescence microscopes. Accounting for these features is often critical in quantitatively interpreting microscopy images, especially those gathering information at scal ...

Amer Physical Soc2024

Advances in High-Speed, Multiparametric Atomic Force Microscopy

Santiago Harald Andany

After decades of technological advancements, high-speed atomic force microscopy (HS-AFM) has emerged as a powerful technique for visualizing dynamic processes. At the nanoscale, the AFM provides valuable insights into the sample by sensing minute interacti ...

EPFL2024

Subsurface fluorescence time-of-flight imaging using a large-format single-photon avalanche diode sensor for tumor depth assessment

Claudio Bruschini, Edoardo Charbon, Arin Can Ülkü, Yichen Feng

Significance: Fluorescence guidance is used clinically by surgeons to visualize anatomical and/or physiological phenomena in the surgical field that are difficult or impossible to detect by the naked eye. Such phenomena include tissue perfusion or molecula ...

SPIE Society of Photo-optical Instrumentation Engineers2024

Revealing the Mechanism Underlying 3D-AFM Imaging of Suspended Structures by Experiments and Simulations

Marcos Penedo Garcia

The invention of 3D atomic force microscopy (3D-AFM) has enabled visualizing subnanoscale 3D hydration structures. Meanwhile, its applications to imaging flexible molecular chains have started to be experimentally explored. However, the validity and princi ...

Wiley-V C H Verlag Gmbh2024

Mitochondrial nanomotion measured by optical microscopy

Sandor Kasas, María Inés Villalba, Priyanka Parmar

Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and de ...

Frontiers Media SA2023

Practical computational imaging by use of spatiotemporal light modulation: from simulations to applications in biological microscopy

François Thierry M Marelli

Optical microscopy is an essential tool for biologists, who are often faced with the need to overcome the spatial and temporal resolution limitations of their devices to capture finer details. As upgrading imaging hardware is expensive, computational metho ...

EPFL2023

Time-Resolved Scanning Ion Conductance Microscopy and Single-Molecule Spectroscopy

Samuel Mendes Leitão

The evolution of the 3D morphology is at the center of many relevant biological processes ranging from cellular differentiation to cancer invasion and metastasis. Microscopy techniques, such as electron microscopy (EM), super-resolution (SR) optical micros ...

EPFL2023

Simple optical nanomotion method for single-bacterium viability and antibiotic response testing

Sandor Kasas, María Inés Villalba, Allan Bonvallat, Eugenia Rossetti

Antibiotic resistance is nowadays a major public health issue. Rapid antimicrobial susceptibility tests (AST) are one of the options to fight this deadly threat. Performing AST with single-cell sensitivity that is rapid, cheap, and widely accessible, is ch ...

National Academy of Sciences2023

Image scanning microscopy with a doughnut beam: signal strength and integrated intensity

Giorgio Tortarolo

We discuss the effects of image scanning microscopy using doughnut beam illumination on the properties of signal strength and integrated intensity. Doughnut beam illumination can give better optical sectioning and background rejection than Airy disk illumi ...

Optica Publishing Group2023

A diamond spin qubit scanning probe microscope

Dinesh Kevin Mathais Pinto

The properties of a physical system only exist in relation to its environment. This thesis presents the development of a novel spin qubit scanning probe microscope operating over a wide range of environmental conditions. The qubit, which sits at the heart ...

EPFL2023

Towards near-atomic resolution imaging of microsecond nanoscale dynamics

Gabriele Bongiovanni

Time-resolved electron microscopy has made significant progress in recent years, with some groups now working on instruments that offer attosecond temporal resolution. While much of the research in the field revolves around the improvement of temporal reso ...

EPFL2023

Advanced Techniques in Optical Diffraction Tomography

Ahmed Bassam Sayed Ayoub Mohamed Emam

In this thesis, we study the 3 challenges described above. First, we study different reconstruction techniques and assess the fidelity of each reconstruction results by means of structured illumination and phase conjugation. By reconstructing the 3D refrac ...

EPFL2022

Optical microscopy gets down to angstroms

Suliana Manley, Giorgio Tortarolo

Super-resolution microscopy at angstrom precision could pave the way to optical structural biology in cells. ...

NATURE PORTFOLIO2022

Application of Copper Thermal Spraying for Electrical Joints between Superconducting Nb3Sn Cables

Pierluigi Bruzzone, Vincenzo D'Auria

This manuscript reports on the application of copper thermal spraying in the manufacturing process of an electrical connection between Nb3Sn cables for superconducting magnets of fusion reactors. The joint is realized through diffusion bonding of the spray ...

MDPI2022

Pitfalls in the spectral measurements of polarization-altering metasurfaces

Olivier Martin, Hsiang-Chu Wang

The optical characterization of metasurfaces and nanostructures that alter the polarization of light is tricky and can lead to unphysical results, such as reflectance beyond unity. We track the origin of such pitfalls to the response of some typical optica ...

Optica Publishing Group2022

Load cell with positive, negative and zero stiffness operating modes for milli- to nanonewton contact force monitoring in electrical probing

Michal Stanislaw Smreczak

Monolithic integrated circuits (ICs) have been miniaturized over the past five decades, and today their components range in size from hundreds of microns to several nanometers. Making point contact with electrical samples under a microscope is referred to ...

EPFL2022

QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy

Andrea Bassi, Antje Keppler, Arne Seitz

A modern day light microscope has evolved from a tool devoted to making primarily empirical observations to what is now a sophisticated , quantitative device that is an integral part of both physical and life science research. Nowadays, microscopes are fou ...

WILEY2021