Publication

Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy

Publications associées (31)

Phase Reconstruction of Low-Energy Electron Holograms of Individual Proteins

Klaus Kern, Stephan Rauschenbach, Sven Alexander Szilagyi, Hannah Julia Ochner

Low-energy electron holography (LEEH) is one of the few techniques capable of imaging large and complex three-dimensional molecules, such as proteins, on the single molecule level at subnanometer resolution. During the imaging process, the structural infor ...

AMER CHEMICAL SOC2022

Lock-in amplified differential phase contrast

Christophe Moser, Damien Claude-Marie Loterie, Timothé Laforest, Chiara Bonati

We introduce a lock-in method to increase the phase contrast in incoherent Differential Phase Contrast (DPC) imaging. The use of a smart pixel detector with in-pixel signal demodulation, paired with synchronized illumination, provides the basis of a bit-ef ...

SPIE-INT SOC OPTICAL ENGINEERING2022

Nonlinear Inverse Problems in Quantitative Phase Imaging

Thanh-An Michel Pham

The topic of this thesis is the development of new algorithmic reconstruction methods for quantitative phase imaging (QPI). In the past decade, advanced QPI has emerged as a valuable tool to study label-free biological samples and uncover their 3D structur ...

EPFL2022

Stain-free identification of cell nuclei using tomographic phase microscopy in flow cytometry

Demetri Psaltis, Joowon Lim, Daniele Pirone

The accurate identification of the three-dimensional quantitative shape of a cell nucleus is now possible without fluorescent staining by applying computational segmentation to refractive index tomograms recorded in the flow cytometry mode. ...

Nature Publishing Group2022

Optical microscopy gets down to angstroms

Suliana Manley, Giorgio Tortarolo

Super-resolution microscopy at angstrom precision could pave the way to optical structural biology in cells. ...

NATURE PORTFOLIO2022

Multicore fiber endoscope for phase imaging based on intensity recording using deep neural networks

Navid Borhani, Demetri Psaltis, Christophe Moser, Eirini Kakkava

The present invention is related to an endoscopic system for phase imaging, comprising a multicore waveguide (080), an optical system comprising at least one first light source (002), for illuminating the sample to be examined, a first camera (010) that is ...

2022

Lock-in incoherent differential phase contrast imaging

Christophe Moser, Damien Claude-Marie Loterie, Timothé Laforest, Chiara Bonati

We introduce a lock-in method to increase the phase contrast in incoherent differential phase contrast (DPC) imaging. This method improves the phase sensitivity by the analog removal of the background. The use of a smart pixel detector with in-pixel signal ...

CHINESE LASER PRESS2022

Method and light microscope with a plurality of arrays of photon-counting detector elements

Claudio Bruschini, Edoardo Charbon, Ivan Michel Antolovic

A method for operating a light microscope comprises emitting and guiding a plurality of illumination light beams towards a specimen (6) to form a plurality of separated illumination light spots (2A, 2B, 2C, 2D) at the specimen; and guiding detection light ...

2022

Optical Diffraction Tomography Using Nearly In-Line Holography with a Broadband LED Source

Demetri Psaltis, Ahmed Ayoub, Abhijit Roy

We present optical tomography methods for a 3D refractive index reconstruction of weakly scattering objects using LED light sources. We are able to record holograms by minimizing the optical path difference between the signal and reference beams while sepa ...

MDPI2022

Application of Copper Thermal Spraying for Electrical Joints between Superconducting Nb3Sn Cables

Pierluigi Bruzzone, Vincenzo D'Auria

This manuscript reports on the application of copper thermal spraying in the manufacturing process of an electrical connection between Nb3Sn cables for superconducting magnets of fusion reactors. The joint is realized through diffusion bonding of the spray ...

MDPI2022

Magnetic force microscopy contrast formation and field sensitivity

Johannes Schwenk, Marcos Penedo Garcia

The magnetic force microscope (MFM) is an established experimental tool for imaging stray fields with high spatial resolution and sensitivity. The MFM contrast can however contain contributions from the sample topography, variations in the surface Kelvin p ...

2022